SHOULD BE IN HEMA XXXRETICULOCYTE COUNT

Question 1

To detect the presence of RNA, the red blood cells must be stained while they are living and this process is called

Answer 1

supravital staining

Question 2

GIVE THE ORDER OF ERYTHOCYTE FORMATION STARTING FROM PRONORMOBLAST

Answer 2

PRONORMOBLAST
BASOPHILIC NORMOBLAST
POLYCHROMATOPHILIC NOMOBLAST
ORTHOCHROMATIC NORMOBLAST
POLYCHROMATOPHILIC ERYTHROCYTE
ERYTHROCYTE

Question 3

The number of reticulocytes in how many red blood cells is determined.

Answer 3

1,000

Question 4

what are the materials used in reticulocyte count

Answer 4

glass slides
anticoagulated blood - edta
supravital stain
automatic pipette
microscope
cedarwood oil

Question 5

procedure for reticulocyte count

Answer 5

Place three drops of reticulocytes stain in a small test tube.
Add three drops of well mixed whole blood to the tube containing the stain.
Mix the tube and allow to stand at room temperature or incubate at 37C for 15 minutes
After 15 minutes, mix the contents of the tube well and prepare smears and allow it to dry.
Examine the smear under the oil immersion objective.
Count the total number of reticulocytes found per 1,000 RBCs

Question 6

formula fpr reticulocyte count

Answer 6

RETICULOCYTE COUNT (%) = (Number of Reticulocytes Counted / 1,000) x 100

Question 7

reference range for retics count

Answer 7

Adult: 0.5-1.5%
Newborn: 2.0-6.0%

Question 8

why do we need to use supravital stains?

Answer 8

dyeing while the cells are in the living state

Question 9

what is the significance of the reticulocyte count

Answer 9

it assesses the erythropoietic activity of the bone marrow to detect underlying diseases such as thalassemia or aplastic anemia

Question 10

what are the 3stains used in supravital staining of retics?

Answer 10

New methylene blue
Pure azure blue
Brilliant cresyl blue

Question 11

the most common among the supravital stain as it stains the retics deeply and uniform

Answer 11

NEW METHYLENE BLUE

Question 12

substitute or 2nd option if the new methylene blue is not avaiablable

Answer 12

pure azure blue

Question 13

least used supravital stain for retics

Answer 13

Brilliant cresyl blue

Question 14

upon staining using supravital the rbc will appear what color?

Answer 14

bluish green

Question 15

upon staining using supravital the rna of the retics will appear

Answer 15

blue

Question 16

why edta is the ideal tube top?

Answer 16

it is stable for the span of 24 hrs, however, the retics will decrease within 6-8 hrs after collection

Question 17

3 components that are sometimes being misidentified as retics

Answer 17

pappenheimer
heinz bodies
howell jolly

Question 18

why papperheimer is being misidentified as a retic?

Answer 18

because of the presence of iron that appears like rna after staining

Question 19

why heinz bodies are being misidentified as a retic/

Answer 19

it composed of denatured rrbc

Question 20

why howell jolly is being identified as a retic

Answer 20

it has the remnants of rna

Question 21

what are the 2 interferences of retics count?

Answer 21

heparin and the high blood glucose level

Question 22

why high blood glucose level is included in the interferences of retics count?

Answer 22

will cause pale staining to the supravital stain

Question 23

conditions where retics are found elevated or high.

Answer 23

• hemolytic anemia
• IDA
• thalassemia
• sideroblastic anemia

Question 24

conditions where retics are found low or decrease

Answer 24

• aplastic anemia
• BM failure
• kidney failure