finals - vaginal secretions Flashcards
one of the most common conditions diagnosed by healthcare providers for female patients particularly women of childbearing age
vaginitis
gold standard for vaginal examination
gram stain
in swabbing vaginal area, why should we not use cotton and wooden shaft for swab
cotton is toxic to neisseria gonorrhoeae
the wood is toxic to chlamydia trachomatis
and calcium alginate can inactivate herpes simplex virus (culture)
an alternative technique to analyze a sample aside from dislodging it to a saline is
dilute the vaginal sample with1-2 drops of saline on the other half of the slide then do the same thing on the right side with 10% KOH
remember the mycv activity? parang ganon
then cover them both with cover slip and observe under the microscope
if the testing is delayed, what is the temp for trichomonas vaginalis and N. gonorrhoeae
Room temp
`
if the testing is delayed, what is the temp for C. trachomatis and herpes simplex virus
refrigerated to prevent the overgrowth of normal flora `
T. Vaginalis must be examined within how many hrs
2 hrs -
palatandaan:
diba pwede tong makita sa urine? 2 hrs lang max ng urine so 2 hrs lang din to hehe
normal appearance of a normal vagigi secretions
white flocculent discharge
normal ph of a normal vagigi secretions
3.8 to 4.2
normal anine (whiff test)of a normal vagigi secretions
negative
normal wbcs of a normal vagigi secretions
2+
normal lactobacilli of a normal vagigi secretions
predominant
normal clue of a normal vagigi secretions
absent
normal other cells of a normal vagigi secretions
absent (except rbc during mens)
normal organism of a normal vagigi secretions
other lactobacilli subgroups, occasionally yeast
this parameter is conducted before submerging the sample in KOH or saline
pH
the pH test helps to differentiate the cause of vaginitis
for vulvovaginal candidiasis, the pH must be
3.8-4.5
for vulvovaginal candidiasis, the pH must be 3.8 -4.5
what will be the pH for bacterial vaginosis, trichomoniasis, desquamative inflammatory vaginitis, and athrophic vaginitis
above 4.5
normal vaginal flora has predominant lactobacilli, this bacteria produces an end product of ___ from __ metabolism
lactic acid from glycogen metabolism
what is the role of lactic acid for vaginal health
provides an acidic environment and maintains the balance of normal flora
aside from lactic acid, other subgroups of lactobacilli can produce ____ which is toxic to pathogen and provides protection from infection
hydrogen peroxide
it’s been associated with the lack of hydrogen peroxide-producing lactobacilli
bacterial vaginosis
what hormone is necessary to preserve an acidic vaginal environment
estrogen
for vaginal infection microscopic exam, what are the screening test we can conduct
KOH and saline wet mount