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Immunology Block 5 > Serology And Rapid Tests BLOCK 6 > Flashcards

Serology And Rapid Tests BLOCK 6 Flashcards

1
Q

What do serology direct tests target

A

Antigens

+ in viral window

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2
Q

What do serology indirect tests indicate

A

Target antibodies

Negative in viral window

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3
Q

Acute infection

A

Antigen (pathogen), or IgM, or >4 fold increase between acute nad convalescent serum titers

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4
Q

Direct fluorescent antibody test

A

Flourescently labeled antibodies light up specific cells of micro organisms

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5
Q

Indirect fluorescent antibody tests

A

Used to demonstrate the presence of antibodies against a specific Ag

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6
Q

Direct fluorescent antigen

A

ID the antigen

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7
Q

Indirect fluorescent antibody

A

ID antibodies to the antigen

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8
Q

ELISA

A
  • common tool to detect/measure Ag or Ab

- information rich: linked immunosorbant assay

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9
Q

Uses of ELISA

A

Antigen

Antibody

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10
Q

Other name of ELISA

A

EIA

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11
Q

Direct ELISA

A

Probes for antigen with a single labeled antibody

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12
Q

Indirect ELISA

A

Probes for antibodies to an antigen labeled antibody binds to unlabeled antibody

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13
Q

Sandwich ELISA

A

Antigen is sandwiches between two antibodies MOST COMMON

Pregnancy tests

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14
Q

What do indirect ELISAs identify

A

Antibodies

Use reporter enzyme

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15
Q

Purpose of control line on ELISA

A

Tells us the test worked

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16
Q

Agglutination

A

Particulate antigens (rbcs) + antibodies= agglutination

Soluble antigens + bound/particulate antigens=agglutination

17
Q

Examples of ELISA

A

CSF/Urine
Fees
Throat (strep)

18
Q

Serum is serially diluted and fixed antigen concentration is added to each tube (or increasing antigen concentrations are added to a fixed antibody concentration)

A

Precipitin curve

19
Q

High levels of free antibody saturate antigens, but produce very little cross-linked Ag:Ab complexes

A

Antibody excess (prozone)

May test negative!!

20
Q

No free Ag or Ab remains in solution after centrifugation of Ab:Ag complexes. Maximal precipitation

A

Equivalence

21
Q

Need max crosslinking, equal Ag-Ab (equivalence)

A

Lattice theory

22
Q

Why is prozone important?

A

Can have someone who is very sick, and still test negative!!

Antibody titer so high, agglutination test is negative

Immunology Block 5 (11 decks)

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