Schematic Diagram

Question 1

Glucose

Answer 1

Reagent: 1000uL
Sample/Standard: 10uL

Mix and incubate for 10 mins at 37 degrees Celsius.

Measure the change in absorbance of standard and sample against reagent blank

Question 2

TAG

Answer 2

Reagent: 1000uL
Sample/Standard: 10uL

Mix and incubate for 5 mins at 37 degrees Celsius.

Measure the change in absorbance of standard and sample against reagent blank

Question 3

Cholesterol

Answer 3

Reagent: 1000uL
Sample/Standard: 10uL

Mix and incubate for 5 mins at 37 degrees Celsius.

Measure the change in absorbance of standard and sample against reagent blank

Question 4

HDL

Answer 4

Reagent: 450uL
Calibrator: 5uL
Sample/Standard: 5uL

Mix and incubate for 5 min at 37 degrees Celsius.

Reagent: 150 ul
Calibrator: 150 uL
Sample: 150 uL

Mix and incubate for 5 min at 37 degrees Celsius and read absorbance at 578-630nm

Question 5

Creatinine

Answer 5

Reagent: 1000uL
Sample/Standard: 100uL

Mix and read the optical density 60 secs after the sample or standard addition.

Exactly 60 secs after the first reading, take the 2nd reading.

Question 6

Urea

Answer 6

Reagent: 1000uL
Sample/Standard: 10uL

Mix and read the optical density 30 secs after the sample or standard addition.

Exactly 60 secs after the first reading, take the 2 ndreading

Question 7

Uric acid

Answer 7

Reagent: 1000uL
Sample/Standard: 25uL

Mix and incubate for 5 mins at 37 degrees Celsius

Question 8

Bilirubin

Answer 8

Total bilirubin rgt: 1000uL
Activator: 20uL
Serum: 50uL

Mix well and incubate for 5 minute at room temperature.

Measure the absorbance of calibrator and test against respective blank at 546nm.

Question 9

Albumin

Answer 9

Reagent: 1000uL
Standard: 10uL

Mix and incubate for 1 minute.

Measure absorbance of standard and sample against the reagent blank.