Agappe NPNs Flashcards
CREATININE
- This reagent Is Intended for in vitro quantitative determination of Creatinine in_____ or _____.
- Modified _______
- Linear up to______
- No Bilirubin interference up to_____
serum plasma and urine
Jaffe’s Method
24 mg/dL
10 mg/dL
Modified Jaffe’s method is used for
Creatinine
_______ Is formed in muscles from_______ .
It Is an Important form of energy, being a store of high-energy phosphate.
Creatinine
phospho creatine
Creatinine determinations have one advantage over____ determination that it is not affected by a high protein diet.
Urea
is more specife and sensitive indicator of renal function.
Serum creatinine
Serum urea nitrogen, creatinine ratio Is > 15 in_____
< 10 In____
Decreased levels are found in______.
pre renal failure
renal failure
muscle dystrophy
Decreased levels are found in muscle dystrophy.
Creatinine
CREATININE
Principle (Jaffe creatinine method)
Creatinine reacts with_____ to produce a colored compound,______.
The change in absorbance is proportional to the creatinine
concentration
picric acid
creatinine alkaline picrate
Creatinine Base Reagent (R1)
Sodium hydroxide - 300 mmol/L
Sodium phosphate - 25 mmol/ L
Creatinine Dye Reagent (R2)
Picric acid- 8.73 mmol/ L
Surfactant
CREATININE
Sample
SPU
Serum
Plasma (free of hemolysis)
Urine (diluted 1/100 with distiled water)
CREATININE
Interferences
No Interference for
Ascorbic Acid up to
Hemoglobin up to
Bilirubin up to
50 mg/dL
1000 mg/dL
10 mg/dL
CREATININE
Wavelength
492 nm/505 nm
CREATININE
Sample Volume
Reagent Volume
100 uL
1000 uL
CREATININE
Traditional to SI
x 88.4
Intended Use
This reagent is intended fot in vitro quantitative determination of urea in serum, plasma & urine.
~_____ / GLDH methodology
- Linear up to____
- Working reagent can he prepared as per requirement
Urease
300 mg/dL
UREA
Clinical Significance
_____ connot be stored in human body, so excess should be broken down.
Amino acids which from the components of proteins, break down to give_____. This is toxic & so through a series of chemical reactions (_____) non toxic urea Is produced & this is released into the blood which is filtered in the kidney & excreted in the urine.
Proteins
ammonia
urea cycle
UREA
Elevated levels are seen during increased____ breakdown, dehydration, vomiting, diarrhea.
Also seen in any kind of renal disorder like Glomerular nephritis.
Chronic nephritis and Nephritic syndrome.
protein
Decreased levels of urea are found in
liver failure
pregnancy
Urea R1
Tris Buffer (pH 7.60) - 100 mmol/L
ADP 0.7 mimol/L a-ketoglutarate - 9.0 mmol/L. (adenine dinucleotide phosphate)
Urease > 6500 U/L
GLDH > 1100 U
Urea R2
NADH 0.25 mmol/L (SL) R2
2-Oxelutarate 5 mmol/L
nicotinamide adenine dinucleotide (NAD) + hydrogen (H)
Urea
Reagent Preparation
Mix___ volume of Reagent I (RI) with___ volume of Reagent 2 (R2)
Working reagent is stable for 30 days at_____
4
1
2-8°C
Urea
Reagent Storage
The sealed reagents are stable up to the expiry date stated on the label, when stored at ____and protected from light.
2- 8 C
UREA
Sample
Serum, Plasma (free of hemolysis)
Do not use____ or ____
fluoride or ammonim
Urea
Interferences
No Interference for
Blirubin up to
Ascorbid acid up to
Hemoglobin up to
20 mg/dL
50 mg/ dL
1000 mg/dL
UREA
Conversion from Traditional to SI
mg/dL
mmol/L
X 0.1665
Urea
Reference Range
It is recommended that each laboratory establish its own reference values.
The following value may be used as guide line.
Serum/ plasma- 10-50mg/dL
Urine - 20-25gm/ 24hr
Intended Use
This reagent is Intended for in vitro quantitative determination of uric acid in serum, plasma & urine.
- _____Method
- Linear up to____mg/dL
- Fast Incubation, Just 5 minutes at 37°C
URID ACID test
Uricase PAP
25 mg/
is the end product of purine metabolism.
Uric acid in excreted by the____.
Uric acid
kidneys
Increased levels are found in Gout, arthritis, trpaired renal functions & starvation.
Decreased levels are found in yellow atrophy of the livet.
Uric acid
Clinical Significance
Urle acid is the end product of purine metabolism.
Uric acid in excreted by the kidneys. Increased levels are found in Gout, arthritis, trpaired renal functions & starvation.
Decreased levels are found in yellow atrophy of the livet.
Uric acid
Conversion from Traditional to SI
× 0.059
Uric acid
Principle
______ transforms uric acid into allantoin, with formation of hydrogen peroxide
Uricase
Uric Acid
Sample
Serum
EDTA or Heparin plasma (free of hemolysis)
Urine (1/10 diluted with distilled woter).
T or F
Creatinine is not affected by diet
True
Enzyme in Urea R1
Urease
Picric acid mmol/ L
8.73 mmol/L
Sodium hydroxide -
Sodium phosphate -
300 mmol/L
25 mmol/ L
