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CC LAB > Agappe NPNs > Flashcards

Agappe NPNs Flashcards

1
Q

CREATININE

  • This reagent Is Intended for in vitro quantitative determination of Creatinine in_____ or _____.
  • Modified _______
  • Linear up to______
  • No Bilirubin interference up to_____
A

serum plasma and urine

Jaffe’s Method

24 mg/dL

10 mg/dL

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2
Q

Modified Jaffe’s method is used for

A

Creatinine

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3
Q

_______ Is formed in muscles from_______ .

It Is an Important form of energy, being a store of high-energy phosphate.

A

Creatinine

phospho creatine

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4
Q

Creatinine determinations have one advantage over____ determination that it is not affected by a high protein diet.

A

Urea

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5
Q

is more specife and sensitive indicator of renal function.

A

Serum creatinine

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6
Q

Serum urea nitrogen, creatinine ratio Is > 15 in_____
< 10 In____

Decreased levels are found in______.

A

pre renal failure

renal failure

muscle dystrophy

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7
Q

Decreased levels are found in muscle dystrophy.

A

Creatinine

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8
Q

CREATININE

Principle (Jaffe creatinine method)
Creatinine reacts with_____ to produce a colored compound,______.

The change in absorbance is proportional to the creatinine
concentration

A

picric acid

creatinine alkaline picrate

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9
Q

Creatinine Base Reagent (R1)

A

Sodium hydroxide - 300 mmol/L
Sodium phosphate - 25 mmol/ L

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10
Q

Creatinine Dye Reagent (R2)

A

Picric acid- 8.73 mmol/ L
Surfactant

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11
Q

CREATININE

Sample

A

SPU

Serum
Plasma (free of hemolysis)
Urine (diluted 1/100 with distiled water)

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12
Q

CREATININE

Interferences
No Interference for

Ascorbic Acid up to
Hemoglobin up to
Bilirubin up to

A

50 mg/dL
1000 mg/dL
10 mg/dL

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13
Q

CREATININE

Wavelength

A

492 nm/505 nm

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14
Q

CREATININE

Sample Volume

Reagent Volume

A

100 uL

1000 uL

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15
Q

CREATININE

Traditional to SI

A

x 88.4

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16
Q

Intended Use
This reagent is intended fot in vitro quantitative determination of urea in serum, plasma & urine.
~_____ / GLDH methodology
- Linear up to____
- Working reagent can he prepared as per requirement

A

Urease

300 mg/dL

17
Q

UREA

Clinical Significance
_____ connot be stored in human body, so excess should be broken down.

Amino acids which from the components of proteins, break down to give_____. This is toxic & so through a series of chemical reactions (_____) non toxic urea Is produced & this is released into the blood which is filtered in the kidney & excreted in the urine.

A

Proteins

ammonia

urea cycle

18
Q

UREA

Elevated levels are seen during increased____ breakdown, dehydration, vomiting, diarrhea.

Also seen in any kind of renal disorder like Glomerular nephritis.

Chronic nephritis and Nephritic syndrome.

A

protein

19
Q

Decreased levels of urea are found in

A

liver failure

pregnancy

20
Q

Urea R1

A

Tris Buffer (pH 7.60) - 100 mmol/L

ADP 0.7 mimol/L a-ketoglutarate - 9.0 mmol/L. (adenine dinucleotide phosphate)

Urease > 6500 U/L

GLDH > 1100 U

21
Q

Urea R2

A

NADH 0.25 mmol/L (SL) R2
2-Oxelutarate 5 mmol/L

nicotinamide adenine dinucleotide (NAD) + hydrogen (H)

22
Q

Urea

Reagent Preparation

Mix___ volume of Reagent I (RI) with___ volume of Reagent 2 (R2)

Working reagent is stable for 30 days at_____

A

4

1

2-8°C

23
Q

Urea

Reagent Storage
The sealed reagents are stable up to the expiry date stated on the label, when stored at ____and protected from light.

A

2- 8 C

24
Q

UREA

Sample
Serum, Plasma (free of hemolysis)

Do not use____ or ____

A

fluoride or ammonim

25
Q

Urea

Interferences
No Interference for

Blirubin up to
Ascorbid acid up to
Hemoglobin up to

A

20 mg/dL
50 mg/ dL
1000 mg/dL

26
Q

UREA

Conversion from Traditional to SI
mg/dL
mmol/L

A

X 0.1665

27
Q

Urea

Reference Range
It is recommended that each laboratory establish its own reference values.

The following value may be used as guide line.

A

Serum/ plasma- 10-50mg/dL

Urine - 20-25gm/ 24hr

28
Q

Intended Use
This reagent is Intended for in vitro quantitative determination of uric acid in serum, plasma & urine.
- _____Method
- Linear up to____mg/dL
- Fast Incubation, Just 5 minutes at 37°C

A

URID ACID test

Uricase PAP

25 mg/

29
Q

is the end product of purine metabolism.

Uric acid in excreted by the____.

A

Uric acid

kidneys

30
Q

Increased levels are found in Gout, arthritis, trpaired renal functions & starvation.

Decreased levels are found in yellow atrophy of the livet.

A

Uric acid

31
Q

Clinical Significance
Urle acid is the end product of purine metabolism.
Uric acid in excreted by the kidneys. Increased levels are found in Gout, arthritis, trpaired renal functions & starvation.
Decreased levels are found in yellow atrophy of the livet.

A

Uric acid

32
Q

Conversion from Traditional to SI

A

× 0.059

33
Q

Uric acid

Principle
______ transforms uric acid into allantoin, with formation of hydrogen peroxide

A

Uricase

34
Q

Uric Acid

Sample

A

Serum
EDTA or Heparin plasma (free of hemolysis)
Urine (1/10 diluted with distilled woter).

35
Q

T or F

Creatinine is not affected by diet

A

True

36
Q

Enzyme in Urea R1

A

Urease

37
Q

Picric acid mmol/ L

A

8.73 mmol/L

38
Q

Sodium hydroxide -
Sodium phosphate -

A

300 mmol/L

25 mmol/ L

CC LAB (46 decks)

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