Agappe NPNs

Question 1

CREATININE

• This reagent Is Intended for in vitro quantitative determination of Creatinine in_____ or _____.
• Modified _______
• Linear up to______
• No Bilirubin interference up to_____

Answer 1

serum plasma and urine

Jaffe’s Method

24 mg/dL

10 mg/dL

Question 2

Modified Jaffe’s method is used for

Answer 2

Creatinine

Question 3

_______ Is formed in muscles from_______ .

It Is an Important form of energy, being a store of high-energy phosphate.

Answer 3

Creatinine

phospho creatine

Question 4

Creatinine determinations have one advantage over____ determination that it is not affected by a high protein diet.

Answer 4

Urea

Question 5

is more specife and sensitive indicator of renal function.

Answer 5

Serum creatinine

Question 6

Serum urea nitrogen, creatinine ratio Is > 15 in_____
< 10 In____

Decreased levels are found in______.

Answer 6

pre renal failure

renal failure

muscle dystrophy

Question 7

Decreased levels are found in muscle dystrophy.

Answer 7

Creatinine

Question 8

CREATININE

Principle (Jaffe creatinine method)
Creatinine reacts with_____ to produce a colored compound,______.

The change in absorbance is proportional to the creatinine
concentration

Answer 8

picric acid

creatinine alkaline picrate

Question 9

Creatinine Base Reagent (R1)

Answer 9

Sodium hydroxide - 300 mmol/L
Sodium phosphate - 25 mmol/ L

Question 10

Creatinine Dye Reagent (R2)

Answer 10

Picric acid- 8.73 mmol/ L
Surfactant

Question 11

CREATININE

Sample

Answer 11

SPU

Serum
Plasma (free of hemolysis)
Urine (diluted 1/100 with distiled water)

Question 12

CREATININE

Interferences
No Interference for

Ascorbic Acid up to
Hemoglobin up to
Bilirubin up to

Answer 12

50 mg/dL
1000 mg/dL
10 mg/dL

Question 13

CREATININE

Wavelength

Answer 13

492 nm/505 nm

Question 14

CREATININE

Sample Volume

Reagent Volume

Answer 14

100 uL

1000 uL

Question 15

CREATININE

Traditional to SI

Answer 15

x 88.4

Question 16

Intended Use
This reagent is intended fot in vitro quantitative determination of urea in serum, plasma & urine.
~_____ / GLDH methodology
- Linear up to____
- Working reagent can he prepared as per requirement

Answer 16

Urease

300 mg/dL

Question 17

UREA

Clinical Significance
_____ connot be stored in human body, so excess should be broken down.

Amino acids which from the components of proteins, break down to give_____. This is toxic & so through a series of chemical reactions (_____) non toxic urea Is produced & this is released into the blood which is filtered in the kidney & excreted in the urine.

Answer 17

Proteins

ammonia

urea cycle

Question 18

UREA

Elevated levels are seen during increased____ breakdown, dehydration, vomiting, diarrhea.

Also seen in any kind of renal disorder like Glomerular nephritis.

Chronic nephritis and Nephritic syndrome.

Answer 18

protein

Question 19

Decreased levels of urea are found in

Answer 19

liver failure

pregnancy

Question 20

Urea R1

Answer 20

Tris Buffer (pH 7.60) - 100 mmol/L

ADP 0.7 mimol/L a-ketoglutarate - 9.0 mmol/L. (adenine dinucleotide phosphate)

Urease > 6500 U/L

GLDH > 1100 U

Question 21

Urea R2

Answer 21

NADH 0.25 mmol/L (SL) R2
2-Oxelutarate 5 mmol/L

nicotinamide adenine dinucleotide (NAD) + hydrogen (H)

Question 22

Urea

Reagent Preparation

Mix___ volume of Reagent I (RI) with___ volume of Reagent 2 (R2)

Working reagent is stable for 30 days at_____

Answer 22

4

1

2-8°C

Question 23

Urea

Reagent Storage
The sealed reagents are stable up to the expiry date stated on the label, when stored at ____and protected from light.

Answer 23

2- 8 C

Question 24

UREA

Sample
Serum, Plasma (free of hemolysis)

Do not use____ or ____

Answer 24

fluoride or ammonim

Question 25

Urea Interferences No Interference for Blirubin up to Ascorbid acid up to Hemoglobin up to

Answer 25

20 mg/dL 50 mg/ dL 1000 mg/dL

Question 26

UREA Conversion from Traditional to SI mg/dL mmol/L

Answer 26

X 0.1665

Question 27

Urea Reference Range It is recommended that each laboratory establish its own reference values. The following value may be used as guide line.

Answer 27

Serum/ plasma- 10-50mg/dL Urine - 20-25gm/ 24hr

Question 28

Intended Use This reagent is Intended for in vitro quantitative determination of uric acid in serum, plasma & urine. - _____Method - Linear up to____mg/dL - Fast Incubation, Just 5 minutes at 37°C

Answer 28

URID ACID test Uricase PAP 25 mg/

Question 29

is the end product of purine metabolism. Uric acid in excreted by the____.

Answer 29

Uric acid kidneys

Question 30

Increased levels are found in Gout, arthritis, trpaired renal functions & starvation. Decreased levels are found in yellow atrophy of the livet.

Answer 30

Uric acid

Question 31

Clinical Significance Urle acid is the end product of purine metabolism. Uric acid in excreted by the kidneys. Increased levels are found in Gout, arthritis, trpaired renal functions & starvation. Decreased levels are found in yellow atrophy of the livet.

Answer 31

Uric acid

Question 32

Conversion from Traditional to SI

Answer 32

× 0.059

Question 33

Uric acid Principle ______ transforms uric acid into allantoin, with formation of hydrogen peroxide

Answer 33

Uricase

Question 34

Uric Acid Sample

Answer 34

Serum EDTA or Heparin plasma (free of hemolysis) Urine (1/10 diluted with distilled woter).

Question 35

T or F Creatinine is not affected by diet

Answer 35

True

Question 36

Enzyme in Urea R1

Answer 36

Urease

Question 37

Picric acid mmol/ L

Answer 37

8.73 mmol/L

Question 38

***Sodium hydroxide*** - ***Sodium phosphate*** -

Answer 38

300 mmol/L 25 mmol/ L