Liver Function Tests

Question 1

Liver Function Tests

–

Answer 1

Hepatic Synthesis Ability –

Conjugation/Excretion Function –

Detoxification Function

Question 2

–Hepatic Synthesis Ability (fukb; erts)
–Total Protein Determination

–

Answer 2

Folin-Ciocalteu (Lowry) Method –
UV Absorption Method
Kjeldahl Method –
Biuret Method –

–Electrophoresis –
Refractometry –
Turbidimetric/Nephelometric Methods – Salt Fractionation

Question 3

–Hepatic Synthesis Ability (3)

Answer 3

–Total Protein Determination
Prothrombin Time –
Albumin

Question 4

–Conjugation/Excretion Function (3)

Answer 4

Bilirubin Assay

–Bromosulfophthalein Test (BSP)

–Urobilinogen

Question 5

Bilirubin Assay

–

Answer 5

Malloy-Evelyn Method –
Jendrassik-Grof Method

Question 6

–Bromosulfophthalein Test (BSP)
Dye Extraction Test

–

Answer 6

Rosenthal White Method –
MacDonald Method

Question 7

–Urobilinogen

–

Answer 7

Ehrlich Method

Question 8

–Detoxification Function

–Enzyme Tests

–

Answer 8

ALP – ALT – AST – 5’N – GGT – LAP – LDH

Question 9

–Detoxification Function

–Ammonia

–

Answer 9

Nesslerization Reaction

Berthelot Reaction

Question 10

Urine bilirubin reacts to diazotized sulfanilic acid to produce red azo dye (540 nm)

Answer 10

Classic Diazo reaction (Ehrlich, 1883)

Question 11

–

–Using serum

–Used alcohol accelerator for the coupling of bilirubin to diazotized sulfanilic acid

Answer 11

Van den Bergh, 1913

Question 12

–
Serum bilirubin –
50% methanol

Answer 12

Malloy-Evelyn Method (1937)

Question 13

Serum bilirubin –
Caffeine-benzoate-acetate

–Ascorbic acid – terminates reaction – alkaline tartrate solution –
B1= TB-B2

Answer 13

Jendrassik-Grof Method (1938)

Question 14

–

–conjugated bilirubin binded to albumin –

seen only in hepatic obstruction

Answer 14

Delta Bilirubin

Question 15

–Advantages of Jendrassik-Grof over Malloy-Evelyn:

–Not affected by____ changes –

Insensitive to a____-fold variation in protein concentration of the sample –

Maintains optical sensitivity even at low bilirubin concentrations –

Has minimal____ and a relatively constant serum blank –

Is not affected by hemoglobin up to ____mg/dL

Answer 15

pH

50

turbidity

750 mg/dL

Question 16

–

– Used in neonatal population – Measurement of reflected light from skin using two wavelengths

– provides numerical index based on spectral reflectance

Answer 16

Bilirubinometry

Question 17

–Yellowishness of the serum or plasma

– 0.01% potassium dichromate

Answer 17

Icterus Index

Question 18

Specimen Requirements

Answer 18

Serum or Plasma

Question 19

Specimen requirement

______is preferred for MalloyEvelyn

– Addition of_____ may precipitate protein – interference!

–_____ sample is preferred

– Presence of____ may interfere

–Protect from____

Answer 19

Serum

alcohol

Fasting

lipemia

light