1. Volumetric 2. Ostwald-Folin 3. Pasteur pipets 4. Automatic macropipets or micropipets

Pipets Flashcards by Kyra Catedral

Used to transfer measured volumes of liquid between containers

PIPETS

How well did you know this?

Not at all

Perfectly

Highly pigmented/colored/opaque (viscous) liquid =

upper meniscus

How well did you know this?

Not at all

Perfectly

Colorless/transparent liquid =

lower meniscus

How well did you know this?

Not at all

Perfectly

Pipets are classified based on:

Design
Drainage characteristics
Type

How well did you know this?

Not at all

Perfectly

Design

A. To contain (TC)
B. To deliver (TD)

How well did you know this?

Not at all

Perfectly

Drainage characteristics

A. Blowout
B. Self-draining

How well did you know this?

Not at all

Perfectly

2 types of pipet

A. Measuring or graduated
B. Transfer

How well did you know this?

Not at all

Perfectly

A. Measuring or graduated

Serologic
Mohr
Bacteriologic
Ball, Kolmer, or Kahn
Micropipet

How well did you know this?

Not at all

Perfectly

B. Transfer

Volumetric
Ostwald-Folin
Pasteur pipets
Automatic macropipets or micropipets

How well did you know this?

Not at all

Perfectly

AKA, “Rinsed-out pipets”

To contain (TC)

How well did you know this?

Not at all

Perfectly

Must be refilled and rinsed-out with the appropriate solvent after the initial liquid has been drained from the pipet

To contain (TC)

How well did you know this?

Not at all

Perfectly

used for dosing the blood which is to be examined.

Quantification of hemoglobin

Sahli-hemoglobin pipets

How well did you know this?

Not at all

Perfectly

TC or TD

Sahli-hemoglobin pipet

How well did you know this?

Not at all

Perfectly

TC or TD

Long-Levy pipets

How well did you know this?

Not at all

Perfectly

Designed to drain by gravity

To deliver (TD)

How well did you know this?

Not at all

Perfectly

Must be held vertically with the tip placed against the side of the container and must NOT TOUCH the liquid in it

To deliver (TD)

How well did you know this?

Not at all

Perfectly

TC or TD

Serologic

How well did you know this?

Not at all

Perfectly

TC or TD

Mohr

How well did you know this?

Not at all

Perfectly

TC or TD

Volumetric transfer pipet

How well did you know this?

Not at all

Perfectly

A_____ pipet holds or contains a particular volume but does not dispense that exact volume, where as a_____ pipet will dispense the volume indicated.

How well did you know this?

Not at all

Perfectly

Must be held vertically with the tip placed against the side of the container and must NOT TOUCH the liquid in it

Exemptions: 2

Serologic and Mohr pipet

How well did you know this?

Not at all

Perfectly

______– calibration marks may be confined to the stem.

With a______, they may extend to the tip.

Mohr pipet

serologic pipette

How well did you know this?

Not at all

Perfectly

Graduated to the tip

Serological pipet

How well did you know this?

Not at all

Perfectly

Not graduated to the tip

Mohr pipet

How well did you know this?

Not at all

Perfectly

S or M Exact 10ml

Serological

S or M No excess

Serological

S or M Calibration mark = 9ml

Serological

S or M Blown out pipet

Serological

S or M TD

Serological

S or M Has excess ml

Mohr

S or M Self draining

Mohr

S or M Apply pressure

Mohr

S or M To contain

Mohr

Self-draining or blowout With a single paint at the top

Self draining

Self-draining or blowout Drain by gravity

Self draining

Self-draining or blowout No - frost -etch -double lines

Self draining

Self-draining or blowout Not graduated to the tip

Self draining

Self-draining or blowout With - double ring - frost - etched

Blowout

Self-draining or blowout Designed to be blown out by pushing a small amount of air out of the pipet, completely emptying it

Blowout

Self-draining or blowout Graduated to the tip

Blowout

Used to measure and transfer a predetermined volume of liquid.

Volumetric/Transfer Pipet

Dispense one volume without further subdivisions

Volumetric/Transfer Pipet

Volumetric/Transfer Pipet Self draining except:

Ostwald-Folin Pasteur

Used for viscous fluid Blowout

Ostwald-Folin

Not good for quantitative analytic techniques; for transferring liquids Not considering specific volumes Medicine dropper-like

Pasteur

Capable of holding a volume greater than 1mL but only one determined volume can be dispensed

Automatic Pipet

The volumetric pipette are always

SELF-DRAINING

Ostwald-Folin pipette are______ (used with biologic fluids having a viscosity greater than that of water)

BLOWN OUT PIPETS

The bulblike enlargement in the pipet stem easily distinguishes the

Ostawd-Folin.

Calibrated to distribute fractional quantity of liquid and principally used for measurements of reagents

Measuring or Graduated pipet

NO graduations to the tip Self-draining

Mohr Pipet

Has Graduation marks to the tip Generally a blown out pipet

Serologic

With a total holding volume of less than 1 mL It may be designed as either a Mohr or serologic pipet

MICROPIPETS

Commonly used in the laboratory Dispense on volume without subdivision (transfer pipet) Also, it can dispense several different volumes just like a measuring pipet

Automatic and Semi-automatic pipet

2 TYPES OF MICROPIPETTE

Air displacement Positive displacement

Micropipette Both are____ operated

piston

AIR DISPLACEMENT Disposable,______ tip

polypropylene

Relies on a piston for creating suction to draw the sample into a disposable tip that must be change after each use

AIR DISPLACEMENT

Piston does not come in contact with the liquid

AIR DISPLACEMENT

Form aerosis that can easily escape the pipet

AIR DISPLACEMENT

POSITIVE DISPLACEMENT Use of_____ tip

capillary (siliconized, glass, plastic)

Operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe

POSITIVE DISPLACEMENT

Does not require a different tip for each use

POSITIVE DISPLACEMENT

Piston comes in contact with the liquid

POSITIVE DISPLACEMENT

- Where we operate to displace the liquid (push button)

PLUNGER

- Press to eject the pipet tip and is connected to the tip ejector

TIP EJECTOR BUTTON

- Allow us to set the volume of liquid to be displace

THUMBWHEEL

- Shows the volume set to displaced

VOLUMETER DISPLAY

pushed in order to aspirate or dispense a particular volume of sample or reagent

PUSH BUTTON

Not going push it but turn it bcs it is going to serve as a large volume adjustment knob In order to adjust the volume to be aspirated in much more larger amounts

PUSH BUTTON

Simply push this down to remove the pipette tips that is found at the bottom portion

TIP EJECTOR BUTTON

Used to adiust the volume to be aspirated in smaller amounts

THUMBWHEEL (FINE VOLUME ADJUSTMENT RING)

Where u are able to determine how much of the solution or what is the exact volume to be aspirated, transferred or measured

VOLUMETER DISPLAY

Connected to the shaft Simply push to move and lead to the removal of your disposable tip

TIP EJECTOR

: mainly for the draining of the entire solution that was aspirated in the pipet tip

Second stop

Automatic pipette that obtain the liquid from a common reservoir and dispense it repeatedly may be bottle-top, motorized, or attached to a dilutor.

Dispensers and dilutor/dispensers -

Although it allows us to have an easier measurement of volumes of samples and reagents, but the aspiration and dispensing of such liquids can still be done manually

Semi-automatic pipettes

It is attached to a machine and a tube is going to be submerged to a particular solution The machine will be regulating the aspiration as well as the dispensing of a particular volume of a reagent ot sample

Automatic pipettes

Automatic pipettes that obtain the liquid from a common reservoir and dispense it repeatedly May be bottle top, motorized, handheld, or attached to a dilutor

Dispensers (Dilutors)