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CC LAB > Pipets > Flashcards

Pipets Flashcards

1
Q

Used to transfer measured volumes of liquid between containers

A

PIPETS

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2
Q

Highly pigmented/colored/opaque (viscous) liquid =

A

upper meniscus

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3
Q

Colorless/transparent liquid =

A

lower meniscus

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4
Q

Pipets are classified based on:

A

Design
Drainage characteristics
Type

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5
Q

Design

A

A. To contain (TC)
B. To deliver (TD)

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6
Q

Drainage characteristics

A

A. Blowout
B. Self-draining

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7
Q

2 types of pipet

A

A. Measuring or graduated
B. Transfer

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8
Q

A. Measuring or graduated

A
  1. Serologic
  2. Mohr
  3. Bacteriologic
  4. Ball, Kolmer, or Kahn
  5. Micropipet
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9
Q

B. Transfer

A
  1. Volumetric
  2. Ostwald-Folin
  3. Pasteur pipets
  4. Automatic macropipets or micropipets
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10
Q

AKA, “Rinsed-out pipets”

A

To contain (TC)

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11
Q

Must be refilled and rinsed-out with the appropriate solvent after the initial liquid has been drained from the pipet

A

To contain (TC)

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12
Q

used for dosing the blood which is to be examined.

Quantification of hemoglobin

A

Sahli-hemoglobin pipets

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13
Q

TC or TD

Sahli-hemoglobin pipet

A

TC

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14
Q

TC or TD

Long-Levy pipets

A

TC

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15
Q

Designed to drain by gravity

A

To deliver (TD)

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16
Q

Must be held vertically with the tip placed against the side of the container and must NOT TOUCH the liquid in it

A

To deliver (TD)

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17
Q

TC or TD

Serologic

A

TD

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18
Q

TC or TD

Mohr

A

TD

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19
Q

TC or TD

Volumetric transfer pipet

A

TD

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20
Q

A_____ pipet holds or contains a particular volume but does not dispense that exact volume, where as a_____ pipet will dispense the volume indicated.

A

TC

TD

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21
Q

Must be held vertically with the tip placed against the side of the container and must NOT TOUCH the liquid in it

Exemptions: 2

A

Serologic and Mohr pipet

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22
Q

______– calibration marks may be confined to the stem.

With a______, they may extend to the tip.

A

Mohr pipet

serologic pipette

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23
Q

Graduated to the tip

A

Serological pipet

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24
Q

Not graduated to the tip

A

Mohr pipet

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25
Q

S or M

Exact 10ml

A

Serological

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26
Q

S or M

No excess

A

Serological

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27
Q

S or M

Calibration mark = 9ml

A

Serological

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28
Q

S or M

Blown out pipet

A

Serological

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29
Q

S or M

TD

A

Serological

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30
Q

S or M

Has excess ml

A

Mohr

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31
Q

S or M

Self draining

A

Mohr

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32
Q

S or M

Apply pressure

A

Mohr

33
Q

S or M

To contain

A

Mohr

34
Q

Self-draining or blowout

With a single paint at the top

A

Self draining

35
Q

Self-draining or blowout

Drain by gravity

A

Self draining

36
Q

Self-draining or blowout

No
- frost
-etch
-double lines

A

Self draining

37
Q

Self-draining or blowout

Not graduated to the tip

A

Self draining

38
Q

Self-draining or blowout

With
- double ring
- frost
- etched

A

Blowout

39
Q

Self-draining or blowout

Designed to be blown out by pushing a small amount of air out of the pipet, completely emptying it

A

Blowout

40
Q

Self-draining or blowout

Graduated to the tip

A

Blowout

41
Q

Used to measure and transfer a predetermined volume of liquid.

A

Volumetric/Transfer Pipet

42
Q

Dispense one volume without further subdivisions

A

Volumetric/Transfer Pipet

43
Q

Volumetric/Transfer Pipet

Self draining except:

A

Ostwald-Folin
Pasteur

44
Q

Used for viscous fluid
Blowout

A

Ostwald-Folin

45
Q

Not good for quantitative analytic techniques; for transferring liquids
Not considering specific volumes
Medicine dropper-like

A

Pasteur

46
Q

Capable of holding a volume greater than 1mL but only one determined volume can be dispensed

A

Automatic Pipet

47
Q

The volumetric pipette are always

A

SELF-DRAINING

48
Q

Ostwald-Folin pipette are______ (used with biologic fluids having a viscosity greater than that of water)

A

BLOWN OUT PIPETS

49
Q

The bulblike enlargement in the pipet stem easily distinguishes the

A

Ostawd-Folin.

50
Q

Calibrated to distribute fractional quantity of liquid and principally used for measurements of reagents

A

Measuring or Graduated pipet

51
Q

NO graduations to the tip
Self-draining

A

Mohr Pipet

52
Q

Has Graduation marks to the tip
Generally a blown out pipet

A

Serologic

53
Q

With a total holding volume of less than 1 mL
It may be designed as either a Mohr or serologic pipet

A

MICROPIPETS

54
Q

Commonly used in the laboratory
Dispense on volume without subdivision (transfer pipet)
Also, it can dispense several different volumes just like a measuring pipet

A

Automatic and Semi-automatic pipet

55
Q

2 TYPES OF MICROPIPETTE

A

Air displacement
Positive displacement

56
Q

Micropipette

Both are____ operated

A

piston

57
Q

AIR DISPLACEMENT
Disposable,______ tip

A

polypropylene

58
Q

Relies on a piston for creating suction to draw the sample into a disposable tip that must be change after each use

A

AIR DISPLACEMENT

59
Q

Piston does not come in contact with the liquid

A

AIR DISPLACEMENT

60
Q

Form aerosis that can easily escape the pipet

A

AIR DISPLACEMENT

61
Q

POSITIVE DISPLACEMENT
Use of_____ tip

A

capillary

(siliconized, glass, plastic)

62
Q

Operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe

A

POSITIVE DISPLACEMENT

63
Q

Does not require a different tip for each use

A

POSITIVE DISPLACEMENT

64
Q

Piston comes in contact with the liquid

A

POSITIVE DISPLACEMENT

65
Q
  • Where we operate to displace the liquid (push button)
A

PLUNGER

66
Q
  • Press to eject the pipet tip and is connected to the tip ejector
A

TIP EJECTOR BUTTON

67
Q
  • Allow us to set the volume of liquid to be displace
A

THUMBWHEEL

68
Q
  • Shows the volume set to displaced
A

VOLUMETER DISPLAY

69
Q

pushed in order to aspirate or dispense a particular volume of sample or reagent

A

PUSH BUTTON

70
Q

Not going push it but turn it bcs it is going to serve as a large volume adjustment knob

In order to adjust the volume to be aspirated in much more larger amounts

A

PUSH BUTTON

71
Q

Simply push this down to remove the pipette tips that is found at the bottom portion

A

TIP EJECTOR BUTTON

72
Q

Used to adiust the volume to be aspirated in smaller amounts

A

THUMBWHEEL (FINE VOLUME ADJUSTMENT RING)

73
Q

Where u are able to determine how much of the solution or what is the exact volume to be aspirated, transferred or measured

A

VOLUMETER DISPLAY

74
Q

Connected to the shaft

Simply push to move and lead to the removal of your disposable tip

A

TIP EJECTOR

75
Q

: mainly for the draining of the entire solution that was aspirated in the pipet tip

A

Second stop

76
Q

Automatic pipette that obtain the liquid from a common reservoir and dispense it repeatedly may be bottle-top, motorized, or attached to a dilutor.

A

Dispensers and dilutor/dispensers -

77
Q

Although it allows us to have an easier measurement of volumes of samples and reagents, but the aspiration and dispensing of such liquids can still be done manually

A

Semi-automatic pipettes

78
Q

It is attached to a machine and a tube is going to be submerged to a particular solution

The machine will be regulating the aspiration as well as the dispensing of a particular volume of a reagent ot sample

A

Automatic pipettes

79
Q

Automatic pipettes that obtain the liquid from a common reservoir and dispense it repeatedly

May be bottle top, motorized, handheld, or attached to a dilutor

A

Dispensers (Dilutors)

CC LAB (46 decks)

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