LiquiCHECK Flashcards
is a major carbohydrate present in the blood & serves as a primary source of energy.
It is usually obtained from ingested starch & sugar.
Glucose
Excessive glucose is stored as inactive_____ mainly in the liver & little in the muscles.
glycogen
Elevated blood glucose levels are found in
diabetes mellitus
hyperthyroidism
hyperadrenalism
certain liver diseases
Decreased glucose levels are found in
Insulinoma
hypothyroidism
hypopituitarism
Glucose reagent R1
pH
Tris Buffer (pH 7.40) 92 mmol/L
Glucose standard concentration
100 mg/dL
Samples for glucose
Serum
plasma (free of hemolysis)
CSF
Temperature
37*C
Glucose
Linearity
600 mg/dL
Glucose
Incubation time
10 mins
Glucose
wavelength
505 (490-550 nm)
Glucose
Glucose Reagent vol.
1000 µL
Glucose
Sample / control vol.
10 µL
Glucose Calculation
Glucose Concentration (mg/dL) =
Absorbance of sample/ Absorbance of standard
x standard conc.
Linearity
This reagent is linear upto 600 mg/dL.
If the concentration is greater than linearity (600 mg/dL)dilute the sample with normal saline and repeat the assay
Glucose
are simple lipids, formed in the liver by glycerol & fatty acids.
Triglycerides
are transported by VLDL, LDL & constitute about 95% of fat, stored as source of energy in the tissue & plasma
Triglycerides
Increased levels are found in hyperlipidemias, diabetes, nephrotic syndrome & hypothyroidism.
Increased levels are risk factor for arteriosclerotic coronary disease, peripheral vascular disease, acute pancreatitis & hyperlipoproteinaemia
TAG
TAG
Decreased levels are found in (2)
malnutrition & hyperthyroidism
2H 2 O2 +4-Aminoantipyrine+ TOPS
(Enzyme)
——— > Violet coloured complex
POD - peroxidase
Triglycerides pH
Pipes–buffer (pH7.00) 5 mmol/L
Triglycerides standard concentration
200 mg/dL
TAG
Standard Concentration
200 mg/dL
TAG
Linearity
1000mg/dL
TAG
Incubation time
5mins
TAG
Incubation time
Sample volume
Reagent volume
5 min
10 µL
1000 µL
TAG
Conversion
Trad -> SI
Traditional Unit - mg/dL
SI Unit - mmol/L
x 0.0114
Triglycerides Con. (mg/dL) =
Absorbance of Sample
————————————x 200 Absorbance of Standard
Glucose unit conversion
mg/dL to mmol/ L
x 0.055
It is the main lipid found in the blood, bile & brain tissues
Cholesterol
It is also one of the most important steroids of the body & is a precursor of many steroid hormones.
Cholesterol
Increased levels are found in hypercholesterolaemia, hyperlipidaemia, hypothyroidism, uncontrolled diabetes, nephritic syndrome & cirrhosis.
Decreased levels are found in malabsorption, malnutrition, hyperthyroidism, anaemia & liver diseases.
Cholesterol
Cholesterol
Cholesterol Reagent
Pipes Buffer (pH 6.70)
50 mmol/L
Cholesterol std. Conc.
200 mg/dL
Cholesterol
Standard Concentration
200 mg/dL
Cholesterol
Incubation time
5mins
Cholesterol
Sample volume
Reagent volume
10 µL
1000 µL
Cholesterol
Unit (Conventional)
mg/dL
Unit (SI)
mmol/L
Conversion to SI unit ______
x0.026
HDL
Linearity
Incubation time
150 mg/dL
5 + 5 min
HDL
Sample Volume
Reagent 1 Volume
Reagent 2 Volume
3 µL
270 µL
90 µL
HDL
Unit Conversion
Traditional Unit
mg / dL
SI Unit
mmol/L
Conversion from Traditional to SI
x 0.026
LDL
Linearity
Incubation time
700 mg/dL
5 +5 min
LDL
Sample Volume
Reagent 1 Volume
Reagent 2 Volume
3 µL
270 µL
90 µL
LDL
Traditional Unit
mg/dL
SI Unit
mmol/L
Conversion from Traditional to SI
x 0.026
