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CC LAB > LiquiCHECK > Flashcards

LiquiCHECK Flashcards

1
Q

is a major carbohydrate present in the blood & serves as a primary source of energy.

It is usually obtained from ingested starch & sugar.

A

Glucose

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2
Q

Excessive glucose is stored as inactive_____ mainly in the liver & little in the muscles.

A

glycogen

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3
Q

Elevated blood glucose levels are found in

A

diabetes mellitus
hyperthyroidism
hyperadrenalism
certain liver diseases

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4
Q

Decreased glucose levels are found in

A

Insulinoma
hypothyroidism
hypopituitarism

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5
Q

Glucose reagent R1

pH

A

Tris Buffer (pH 7.40) 92 mmol/L

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6
Q

Glucose standard concentration

A

100 mg/dL

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7
Q

Samples for glucose

A

Serum
plasma (free of hemolysis)
CSF

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8
Q

Temperature

A

37*C

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9
Q

Glucose

Linearity

A

600 mg/dL

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10
Q

Glucose

Incubation time

A

10 mins

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11
Q

Glucose

wavelength

A

505 (490-550 nm)

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12
Q

Glucose

Glucose Reagent vol.

A

1000 µL

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13
Q

Glucose

Sample / control vol.

A

10 µL

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14
Q

Glucose Calculation

Glucose Concentration (mg/dL) =

A

Absorbance of sample/ Absorbance of standard

x standard conc.

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15
Q

Linearity

This reagent is linear upto 600 mg/dL.

If the concentration is greater than linearity (600 mg/dL)dilute the sample with normal saline and repeat the assay

A

Glucose

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16
Q

are simple lipids, formed in the liver by glycerol & fatty acids.

A

Triglycerides

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17
Q

are transported by VLDL, LDL & constitute about 95% of fat, stored as source of energy in the tissue & plasma

A

Triglycerides

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18
Q

Increased levels are found in hyperlipidemias, diabetes, nephrotic syndrome & hypothyroidism.

Increased levels are risk factor for arteriosclerotic coronary disease, peripheral vascular disease, acute pancreatitis & hyperlipoproteinaemia

A

TAG

19
Q

TAG

Decreased levels are found in (2)

A

malnutrition & hyperthyroidism

20
Q

2H 2 O2 +4-Aminoantipyrine+ TOPS

(Enzyme)

——— > Violet coloured complex

A

POD - peroxidase

21
Q

Triglycerides pH

A

Pipes–buffer (pH7.00) 5 mmol/L

22
Q

Triglycerides standard concentration

A

200 mg/dL

23
Q

TAG

Standard Concentration

A

200 mg/dL

24
Q

TAG

Linearity

A

1000mg/dL

25
Q

TAG

Incubation time

A

5mins

26
Q

TAG

Incubation time

Sample volume

Reagent volume

A

5 min

10 µL

1000 µL

27
Q

TAG

Conversion
Trad -> SI

Traditional Unit - mg/dL
SI Unit - mmol/L

A

x 0.0114

28
Q

Triglycerides Con. (mg/dL) =

A

Absorbance of Sample
————————————x 200 Absorbance of Standard

29
Q

Glucose unit conversion

mg/dL to mmol/ L

A

x 0.055

30
Q

It is the main lipid found in the blood, bile & brain tissues

A

Cholesterol

31
Q

It is also one of the most important steroids of the body & is a precursor of many steroid hormones.

A

Cholesterol

32
Q

Increased levels are found in hypercholesterolaemia, hyperlipidaemia, hypothyroidism, uncontrolled diabetes, nephritic syndrome & cirrhosis.

Decreased levels are found in malabsorption, malnutrition, hyperthyroidism, anaemia & liver diseases.

A

Cholesterol

33
Q

Cholesterol

Cholesterol Reagent

A

Pipes Buffer (pH 6.70)

50 mmol/L

34
Q

Cholesterol std. Conc.

A

200 mg/dL

35
Q

Cholesterol

Standard Concentration

A

200 mg/dL

36
Q

Cholesterol

Incubation time

A

5mins

37
Q

Cholesterol

Sample volume
Reagent volume

A

10 µL

1000 µL

38
Q

Cholesterol

Unit (Conventional)
mg/dL

Unit (SI)
mmol/L

Conversion to SI unit ______

A

x0.026

39
Q

HDL

Linearity

Incubation time

A

150 mg/dL

5 + 5 min

40
Q

HDL

Sample Volume

Reagent 1 Volume

Reagent 2 Volume

A

3 µL

270 µL

90 µL

41
Q

HDL

Unit Conversion

Traditional Unit
mg / dL

SI Unit
mmol/L

Conversion from Traditional to SI

A

x 0.026

42
Q

LDL

Linearity

Incubation time

A

700 mg/dL

5 +5 min

43
Q

LDL

Sample Volume

Reagent 1 Volume

Reagent 2 Volume

A

3 µL

270 µL

90 µL

44
Q

LDL

Traditional Unit
mg/dL

SI Unit
mmol/L

Conversion from Traditional to SI

A

x 0.026

CC LAB (46 decks)

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