SAFETY IN THE CLINICAL LABORATORY Flashcards
What are potentially harmful microorganisms called?
Biological hazards
What are the 6 components of the Chain of Infection?
(IREMES)
Infectious agent, Reservoir, Exit portal, Mode of transmission, Entry portal, Susceptible host
What is required for the Chain of Infection?
(SMS)
Continuous link between:
Susceptible host, Method of transmission, Susceptible host
What are the 3 routes of infection?
Inhalation, ingestion, direct inoculation or skin contact
What is the best way to break the chain of infection?
Hand washing
What is the primary method of infection transmission?
Hand contact
What prevention is used when hands are soiled?
Wash hands with soap and water
What method is used when hands are NOT visibly soiled?
Apply alcohol based hand-rub (sanitizer)
What is the handwashing procedure?
1) Wet hands w/ warm water
2) Apply antimicrobial soap
3) Rub to form lather, create friction to loosen debris
4) Thoroughly clean hands for 15-20 secs
5) Rinse hands in DOWNWARD position
6) Dry with a paper towel
7) Turn off faucets with a clean paper towel to prevent recontamination
What is the most important step in handwashing?
Friction
How long should hands be washed?
At least 15-20 seconds
What is the washing hands position?
DOWNWARD
What is the last step in handwashing?
Turn off faucets with a clean paper towel
Where must all biological waste be placed?
In appropriate labeled containers with biohazard symbol EXCEPT Urine
What is the color of the accepted BIOHAZARD label?
Fluorescent orange
Where should urine be discarded?
Sink
What is used for disinfection of the sink?
1:10 dilution of sodium hypochlorite (NaOH)
How often should disinfection of the sinks be performed?
Every day (daily)
What does disinfection eliminate?
Almost all microorganisms except bacterial spores
How is a 1:10 dilution of NaOH prepared?
1 part NaOH to 9 parts water
How long is a 1:10 dilution of disinfectant effective?
1 month
What is the basic outline of the biohazard symbol?
Trefoil (three circles overlapping each other)
What is the sharp hazard symbol?
Syringe with a NO circle sign
What is the color of the puncture resistant container?
Red
What is the best method of waste disposal for radioactive hazards?
Store the used radioactive material in a locked room, marked room until the background count is down to 10 half-lives for radioiodine
What is the chemical spill symbol?
Skull with cross bones
What is the best first aid for chemical spills?
Flush the area with amounts of water for at least 15 mins
What is the first aid for alkali/acid burns in the eye?
Wash the eye thoroughly with running water for 15 mins
How should acid spills on floors be cleaned up?
Neutralized then soaked up with wet rags or spill pillows
What should be done to avoid sudden splashing when handling chemicals?
ACID TO WATER (explosion can occur when water is added to acid)
What does NFPA stand for?
National Fire Protection Association
What is the chemical hazard symbol color from top (clockwise diamond)?
RYWB (Fire, Reactivity, Specific, Health)
What are the degrees of Hazard Index?
(No SMS Ex)
0 - No/Minimal, 1 - Slight hazard, 2 - Moderate Hazard, 3 - Serious Hazard, 4 - Extreme/ Severe Hazard
What does NFPA Yellow Quadrant: Stable indicate?
0
What does NFPA Yellow Quadrant: Unstable if heated indicate?
1
What does NFPA Yellow Quadrant: Violent chemical change indicate?
2
What does NFPA Yellow Quadrant: Shock and heat may deteriorate indicate?
3
What does NFPA Yellow Quadrant: May deteriorate indicate?
4
What does NFPA Blue Quadrant: Normal material indicate?
0
What does NFPA Blue Quadrant: Slightly hazardous indicate?
1
What does NFPA Blue Quadrant: Hazardous indicate?
2
What does NFPA Blue Quadrant: Extreme danger indicate?
3
What does NFPA Blue Quadrant: Deadly indicate?
4
What does NFPA White Quadrant: OXY indicate?
Oxidizer
What does NFPA White Quadrant: W indicate?
No water
What does NFPA Red Quadrant: Will not burn indicate?
0
What does NFPA Red Quadrant: Above 200 F (93.333 C) indicate?
1
What does NFPA Red Quadrant: Below 200 F (93.3333 C) indicate?
2
What does NFPA Red Quadrant: Below 100 F (37.77 C) indicate?
3
What does NFPA Red Quadrant: Below 73 F (22.78 C) indicate?
4
How is electrical equipment grounded to avoid electric shock?
3 pronged plug
What precautions should be taken when electric shock occurs?
Never touch the person or the equipment involved, Turn off circuit breaker, Unplug equipment, Move the equipment using a nonconductive glass or wood object
Where should flammable chemicals be stored?
In safety cabinets and explosion proof refrigerators
How often must all laboratory personnel be involved in laboratory fire drills?
Annually
What should be done when a fire is discovered (RACE)?
Rescue, Activate fire system, Close all doors, Extinguish/Evacuate
What is the procedure to operate a fire extinguisher (PASS)?
Pull, Aim - at the base, Squeeze, Sweep - side to side
What type of hazard involves ordinary combustibles like paper and wood?
Type A
Extinguisher: Water, Dry chemical, Loaded steam
What type of hazard involves flammable liquids like gasoline?
Type B
Extinguisher: Dry chemical, Carbon dioxide, halon foam
What type of hazard involves electrical equipment?
Type C
Extinguisher: Dry chemical, carbon dioxide, HALON
What type of hazard involves flammable metals?
Type D
Extinguisher: Metal X, Sand, Dry powder, fought by fire fighters only
What type of hazard involves detonation?
Type E
Allowed to burn out and nearby materials are protected
What type of hazard involves cooking media?
Type K
Liquid designed to prevent splashing and cool the fire
What types of fires can dry chemical extinguish?
Type A, B, C
What types of fires can carbon dioxide extinguish?
Type B and C
What is the best type of extinguisher for electric equipment?
Halon
What are the most common all-purpose extinguishers?
Dry chemical
What classes of fires should be handled by trained personnel?
Class D and E
What is an ergonomic hazard?
Work related hazard that involves strain due to repeated position
What hazard is caused by extremely low temperatures?
Cryogenic
What type of hazard involves centrifuge, refrigerators, autoclaves, homogenizers, and glassware?
Mechanical
What can centrifuge accidents produce?
Aerosols