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AUBF > Microscopic Technique > Flashcards

Microscopic Technique Flashcards

1
Q

Recommended volume for urine sediment preparation

A

12 mL

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2
Q

Centrifuge force of urine sediment preparation

A

400 RCF for 5 minutes

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3
Q

Remaining urine decant volume should be at

A

0.5 to 1.0 mL

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4
Q

Sediment volume when transferring to glass slide

A

20 uL or 0.02 mL

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5
Q

Glass slide coverslip measurement

A

22 x 22 mm

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6
Q

If < 12 mL urine is available for microscopy centrifuge

A

3 mL of it

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7
Q

If <3 mL urine is available, examine the urine by

A

without centrifugation

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8
Q

RCF formula

A

1.118 x 10^-5 x radius in centimeters x RPM^2

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9
Q

Quantitative measure of formed elements of urine using

A

Hemacytometer

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10
Q

Normal values of Addis count RBC

A

0-500,000/12 hr urine

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11
Q

Normal values of WBCs and ECs Addis count

A

0-1,800,000/ 12 hr urine

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12
Q

Normal values: Hyaline casts addison count

A

0-5,000/12 hr urine

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13
Q

Located in the objective and is adjusted to be near the specimen

A

First lens system

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14
Q

Located in the eyepiece (ocular lens)

A

Second lens system

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15
Q

Ability to distinguish 2 small objects that are a specific distance apart

A

Resolution

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16
Q

Microscopes requiring minimum adjustment when switching objectives

A

Parfocal

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17
Q

Used to remove dust on the optical surface of the microscope

A

Camel hair brush

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18
Q

Used to clean the optical surfaces of the microscope

A

Lens paper

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19
Q

Used to clean any contaminated lens

A

Commercial lens cleaner

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20
Q

To remove oil on lens

A

use dry lens paper > lens paper moistened w/ lens cleaner

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21
Q

Consists of a base, body tube, and nosepiece

22
Q

Performs initial focusing used for LPO and SO

A

Coarse adjustment Knob

Moves stage noticeably up and down, bringing slide into view (vertical movement)

23
Q

Focuses and controls the light on the specimen

A

Condenser

Aperture diaphragm controls the amount and angle of light

24
Q

Centers the field of view

A

Condenser Centering Screws

25
Q

Adjust size opening of condenser

A

Condenser control ring

26
Q

Moves condenser up and down to focus light on the object

A

Condenser Focus Knob

27
Q

Rotate to adjust for interpupillary distance

A

Oculars (eyepiece)

Further magnifies image 10x (secondary magnification)

28
Q

Controls the diameter of the light beam reaching the slide

A

Field Diaphragm

29
Q

Adjusts size opening of field diaphragm

A

Field Diaphragm control ring

30
Q

Sharpens the image (used of HPO and OIO)

A

Fine adjustment knob

31
Q

Adjusts distance between oculars horizontally

A

Interpupillary Distance Control

32
Q

Lenses which form primary (initial) image of specimen

A

Objectives

33
Q

Use to rotate objectives

A

Revolving nosepiece

34
Q

Regulate intensity of light

35
Q

Holds slide in place

A

Slide holder

36
Q

Holds specimen

37
Q

Loosen to remove stage

A

Stage clamp screw

38
Q

Moves slide on stage

A

Mechanical stage knobs

39
Q

Correct sequence of lenses when light is passing through the microscope starting from the source

A

Light source > Condenser > Stage > Objectives > Oculars

Diaphragm > Condenser > Objective > Ocular

40
Q

Microscope for routine urinalysis

A

Bright Field Microscopy

41
Q

Enhances visualization of translucent elements (low refractive indices; casts)

A

Phase Contrast Microscopy

42
Q

To convert Bright-field to Phase contrast what should be done?

A

replace objective lens and condenser with PC objective lens and PC condenser

43
Q

Detects presence or absence of bifringence

A

Polarizing microscope

Useful for identification of cholesterol in oval fat bodies, fatty casts, and crystals

44
Q

To convert bright-field to polarizing, what should be done?

A

Add 2 filters; 1 below the condenser and 1 between objectives and oculars

45
Q

Microscope for identifying Treponema pallidum

A

Dark field microscope

46
Q

To convert Bright Field to Dark field, what should be done?

A

replace the condenser with a DF condenser that contains an opaque disk

47
Q

Microscope for visualization of fluorescent substances and microorganisms

A

Fluorescence Microscopy

48
Q

3D microscopy image and layer by layer imaging of a specimen

A

Interference-contrast microscopy

49
Q

Differential interference contrast

50
Q

Modulation contrast microscope

51
Q

Specimen for Addis count

A

12 hr urine

52
Q

Preservative for addis count

AUBF (26 decks)

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