RNA Processing

Question 1

eukaryotic mRNA’s are processed during __________

Answer 1

transcription

Question 2

the spliceosome is a _______

Answer 2

ribozyme

Question 3

________ proceeds through two transesterification reactions that do not require outside energy

Answer 3

splicing

Question 4

RNA’s can be chemically modified ________________

Answer 4

post transcriptionally

Question 5

a eukaryotic mRNA has a ___ cap and a ________ tail. they code ____ protein.

Answer 5

5’, poly A, one

Question 6

eukaryotic RNAP II complex recruits RNA modifying complexes to the nascent transcript so RNAs are _______ as they are ________,.

Answer 6

modified, made

Question 7

The ____ terminal end of RNAP II recruits modifying enzymes.

Answer 7

C

Question 8

The 5’ cap is a methylated guanosine added ____________ which protects from ______ and recruits proteins to begin ________.

Answer 8

co transcriptionally, degradation, translation

Question 9

the 3’ poly A tail is added _____ transcription in an untemplated fashion by _________ polymerase.

Answer 9

after, poly A

Question 10

RNA splicing removes ______ from the sequences of pre-mRNA’s and binds ____.

Answer 10

introns, exons

Question 11

the rearrangement of exons allows one gene to encode _________ different proteins.

Answer 11

several

Question 12

intron removal proceeds through two __________ reactions.

Answer 12

transesterfication

Question 13

intron removal
- 5’ splice site is attacked by the ___ OH group of the branch site adenosine residue
- the 3’ splice site is attacked by the newly formed ___ OH group of the upstream _____
- the _____ are joined and the intron is ______

Answer 13

2’, 3’, exon, exon, removed

Question 14

intron splicing is catalyed by RNAs (_______) and is assisted by proteins (__________)

Answer 14

snRNAs, snRNPs

Question 15

splice sites are marked by short __________ sequences.

Answer 15

consensus

Question 16

local context of transcription influences _______ as consensus sequences are not enough

Answer 16

splicing

Question 17

RNA’s can be edited _____ transcriptionally which is normal for tRNAs, and rRNAs and sometimes mRNAs

Answer 17

post

Question 18

rRNA _________ are genetically modfied and cleaved to yield mature rRNAs.

Answer 18

precursors

Question 19

mRNAs can be ____ by base deamination to have their sequence _____

Answer 19

edited, altered

Question 20

goal of sequence editing is often the ____ of double stranded RNA formation as this triggers ________ response.

Answer 20

reduction, immune