DNA Polymerase in Lab vs. Cells Flashcards
DNA Polymerase in the cell has ________ timed replication of long chromosomes, ______ per cycle, and are done at _______ temperature.
precisely, once, same
Because synthesis is 5’ to 3’, replication of complementary DNA strands in the cell is ________ and is ______________.
asymmetric, semi-discontinuous
Many ____________ enzymes are required for fast high ________ replication of genomes such as helicase, DNA primase, and DNA ligase.
accessory, fidelity
In the lab, _______ _______ reaction amplifies short sequences of interest with steps controlled by ______
polymerase chain, temperature
DNA Polymerase active site has _______ , 3’ OH of ______ strand, nucleoside 5’triphosphate, 2Mg+.
template, growing
DNA ________ is the stepwise, directional addition of ______ to a growing DNA chain.
synthesis, dNTPs
DNA synthesis by polymerases require a _______ strand and a _______.
template, primer
DNA strands must be ________ prior to DNA synthesis.
separated
DNA polymerization always occurs in the ___ to ___ direction.
5’ , 3’
DNA double helix must be denatured for _________. In cells, denaturation is localized and starts at an ______ of replication.
replication, origin
A __________ _______ is where DNA replication in cells require many more components than the polymerase.
replication fork
The _________ of DNA strands is an ________-_________ process carried out by special enzymes.
separation, energy dependent
DNA helicases hydrolyze ____ to induce cyclic conformational changes and mechanical forces which are moving wedges that _____ the helix _____.
ATP, pry, apart
_____ _____ ______ proteins bind to the unwound strands of DNA to keep them separate and stop them from forming a helix again.
single strand binding
Because the DNA Double Helix is antiparallel, the synthesis of one DNA strand is __________ whereas it’s complementary strand is synthesized in ________.
continuous, fragments
DNA polymerases need an ____ primer. DNA primase is an ____ polymerase.
RNA, RNA
synthesis of the _______ strand leaves gaps that must be _______ by DNA _______.
lagging, sealed, ligase
DNA _______ seals gaps by charging the 5’ phosphate with ____ in the nick, which is subsequently attacked by 3’OH, sealing the DNA with phosphate.
ligase, AMP
_______ unwind DNA double helix ______ of the replication fork.
helicases, infront
IN PCR, the template is provided by ______ and can vary widely in ______.
you, quality
In PCR, DNA primers are provided by _______ and direct where synthesis ________.
you, starts
In PCR, the DNA strand is separated by ________ but a DNA polymerase that can _________ heat is required.
heating, withstand
PCR amplifies DNA through successive rounds of _________, _________, and __________ all regulated by temperature.
denaturation, annealing, extension
PCR results in the ___________ amplification of DNA molecules.
exponential