Regulation of Transcription + Assays

Question 1

______ transcription is more complex due to more RNA polymerases, more components for each one but the principles are _________.

Answer 1

eukaryotic, conserved

Question 2

transcription is regulated by ______ _______ ______ (activators and repressors) that control RNA polymerase II assembly or progression.

Answer 2

DNA binding proteins

Question 3

common DNA binding _________ include helix turn helix, zinc fingers and leucine zippers

Answer 3

motifs

Question 4

binding can be sequence specific without _______ in eukaryotic

Answer 4

denaturation

Question 5

in eukaryotic cells they are ____________ and transcription and translation are _________

Answer 5

compartmentalized, separated

Question 6

translation occurs in ________ and ________

Answer 6

cytosol, ribosome

Question 7

transcription occurs in ________

Answer 7

nucleus

Question 8

eukaryotic cells have three nuclear ______ ________.

Answer 8

RNA polymerases

Question 9

euk
RNA P I transcribe ______
RNA P II transcribe __________ _______ genes

Answer 9

rRNA genes, protein coding

Question 10

eukaryotic cells also have other RNA polymerases in their ________

Answer 10

organelles

Question 11

RNAP II transcription requires multiple ________ components and general ______ factors

Answer 11

additional, transcription

Question 12

____ position RNA polymerase II, open the DNA, trigger release

Answer 12

TF’s

Question 13

some common elements ________ general transcription factors RNA

Answer 13

recruit

Question 14

________ include BRE, TATA, INR, DPE

Answer 14

elements

Question 15

in promoter recognition, _____ results in DNA bending and ______ has helicase activity to unwind double stranded DNA

Answer 15

TBP(tata), TFIIB (BRE)

Question 16

gene expression is controlled by DNA ________ ________

Answer 16

binding proteins

Question 17

negative regulation involves dissociation or binding of __________

Answer 17

repressor

Question 18

positive regulation involves dissociation or binding of _______

Answer 18

activator

Question 19

proteins can interact with DNA in a sequence specific manner without ___________ the helix

Answer 19

denaturing

Question 20

______ ______ _______ are particularly well suited to H bond with basepairs in the major groove of DNA helix

Answer 20

arginine, glutamine, asparagine

Question 21

the leucine zipper has a ____________ core

Answer 21

hydrophobic

Question 22

in the helix turn helix a recognition helix binds in the ________ groove

Answer 22

major

Question 23

in the zinc finger motif ______ stabilizes fold, interacts with _____ groove and increases _________ with multiple domains.

Answer 23

Zn2+, major, affinity