DNA Double Helix MCDB108 A Flashcards
DNA is carrier of the ____
gene
photograph 51 (crick) determined
helix with _____ dimensions
phosphates on ______
water content = only ____ chains
unit cell indicated ____ strands
layer line spacing = ____ residues per turn
constant, outside, two, antiparallel, 10
watson realized that in ____ form purines could ___ bond with pyrimidines to match chargaff’s rules.
keto, h
the DNA double helix is two chains with _________ backbone and internal _______
phosphodiester, basepairs
one turn in a DNA double helix has __ base pairs which is ___ A
10.5, 36
one base pair is ___ A
3.4
the DNA double helix has two ____ chains with a ___ and _____ groove
antiparallel, minor, major
the DNA double helix can be separated by ____ temp. the denaturation can be observed on graph by an increase in _____ (hyperchromic effect)
increasing, A260
the melting temperature of DNA _____ be predicted by composition. it is dependent on ______ _____ sequence
cannot, base stacking
Tm is best predicted by _________ calculations which account for basepair __ bonding and the stabilization of base stacking interactions
nearest neighbor, h
the sides of base paris are exposed to ____ in the major and minor grooves
solvent
the glycosidic bond will always be facing the ______ groove side.
minor
DNA grooves provide sites of interaction with _______. ___ grooves primarily.
proteins, major
the watson and crick helix (most common DNA-DNA helix) is the right handed ___ form
B
there is a ___ handed A form and a ____ handed Z form
right, left
the DNA double helix can adopt ______ structural forms
various
A form Dna causes the plane of each base pair to ____ by about 20 degrees
tilt
DNA molecules are ______ and _____
flexible, twisty
DNA molecules are typically ___ and pose a packing challenge for the cell
long
RNA can only form double helix in the ___ form due to steric constraints
A
single stranded nucleic acids can adopt elaborate _______
structures
the structural plasticity of ____ enables it to perform functions beyond carrying ______ _____
RNA, genetic information
DNA origami is taking advantage of DNA ________ to build macromolecular structures
complementarity
