Glycoconjugates MCDB108 A Flashcards
sugars attached to proteins make _______
glycoproteins/ proteoglycans
sugars attached to lipids make _______
glycolipids
glycoconjugates are synthesized in the _______ and ________
ER, golgi apparatus
proteoglycans help create the _________ matrix, mediate cell-cell _____ and diversify signaling pathways
extracellular, adhesion
_____ have carbohydrate binding domains that read the sugar code
lectins
proteoglycans have ________ being the larger component by weight
carbohydrates
glycoproteins have _______ being the larger component by weight
proteins
glycosaminoglycans (GAG) have linear repats of ________
disaccharides
repeating disaccharides in GAGs are highly ______ charged
negatively
negative charge leads to extended, hydrated molecules wit high volume/mass ration that form gels at low concentrations which act as ______ ______
shock absorbers
________ are heavily glycosylated proteins (80% carb) that work as lubricants and __________ barriers.
mucins, protective
antibodies have defined _____ essential for their function
glycans
glycosylation of antigens impedes ________ recognition
immune
in the ER a pre built ______ core is built onto a lipid and transferred to _____ residues
saccharide, asparagine
glycans in the ER contribute to _____ folding and ______ control
protein, quality
in the golgi new _____ can be added to serine/threonines, glycans are made more ______, and proteins are sorted to _______
glycans, complex, destination
the golgi harbors many glycosyl transferases which modify N and O linked glycans into complex _______ ideal for signaling
sugars
glycolipids are also modified in the _____ and _____
ER, golgi
a _________ has 1-7 sugars attached to a sphingolipid, and is only found on the extracellular leaflet. it is made in the golgi
cerebroside
blood ABO groups are determined by absence/presence of _________ transferases
glycosyl
different ________ attached to proteins and lipids on the surface of red blood cells distinguish one blood group from another
carbohydrates
the glycans on glycoproteins are recognized by _______ which are specialized proteins that bind _________
lectins, carbohydrates
lectin receptor site structure dictates ________ by h bonding to hydroxyls, hydrophobic interactions with CH, shape, and sterics
selectivity
acid hydrolysis helps break ________ bonds
glycosidic
modification of open hydroxyls before acid hydrolysis using methyl iodide shows ______ involved in __________ linkages.
carbons, glycosidic
sensitivity to endoglycosdiase H is a marker of ______ along the ______ pathway
progression, secretory
