Rizz (signaling) Flashcards
What is sarcoma caused by?
Virus
What happened when researchers ground up sarcoma cells and put it in a healthy chicken?
The chicken got a tumor
How did the researchers find the sarc DNA?
Chromatography
Reverse transcribing wild type RNA into fragments of DNA -> hybridizing with the mutant RNA (binding to it) -> whatever wasn’t paired if the sarc DNA
What is sarc?
A kinase
How do kinases charge things?
Removing a phosphate from ATP and adding it to a protein
What charge does a phosphate group add to a protein when it is added?
Negative
What removes a phosphate from a protein’s side chain?
protein phosphatase
What must be removed from an inactive rasp protein to reactivate it?
GDP and then a GTP can add itself
What must interact with a Ras protein to activate it?
A Ras-activating protein which removes its GDP
What activates RTK (receptor tyrosine kinase)?
A signal molecule
What does RTK activate? At what domain?
An adaptor protein, domain SH2
What domain do adaptor proteins and activating proteins interact?
SH3
What does the adaptor protein do to the activating protein?
It transfers a phosphate to it
What does the Ras protein do once activated?
Starts signal cascade
What is the order is the MAP kinase signaling?
Kinase kinase kinase -> kinase kinase -> kinase -> proteins
