DNA Studies Flashcards

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1
Q

What are the components of nucleic acids?

A

5-carbon sugar, phosphate group, nitrogenous base

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2
Q

What is the difference in the sugar between RNA and DNA?

A

Ribose and Deoxyribose: 2’ carbon on ribose has an OH while 2’ carbon on deoxyribose has an H

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3
Q

What charge is DNA and RNA?

A

Negative

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4
Q

What does the “sugar-phosphate backbone” refer to?

A

Alternating phosphates and sugars which is highly negative

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5
Q

What did the Hershey-Chase experiment prove?

A

DNA is genetic material

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6
Q

In the Hershey-Chase experiment, was the radioactive Phosphate or radioactive Sulfur found within the bacterium?

A

Radioactive Phosphate

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7
Q

What are the two grooves on DNA?

A

Minor (the short one) and major (longer one)

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8
Q

How many hydrogen bonds are between A-T?

A

2

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9
Q

How many hydrogen bonds are in a C-G bond?

A

3

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10
Q

What is a purine? What are the two purines?

A

A base with the six member ring and a pentagon ring. Adenine and guanine.

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11
Q

What is a pyrimidine? What are the two pyrimidines?

A

A six member ring base. Cytosine and thymine.

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12
Q

What difference does the OH make on the RNA as opposed to DNA?

A

It makes it less stable and much more reactive

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13
Q

What are enzyme-like RNAs called?

A

Ribozymes

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14
Q

What is a common structure observed with single strand RNA?

A

Hairpin

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15
Q

What structure is found at the 3’ end on DNA/RNA?

A

OH group

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16
Q

What structure is found at the 5’ end?

A

Phosphate group

17
Q

Is DNA replication semiconservative, conservative, or dispersive?

A

Semiconservative

18
Q

What did the Meselson-Stahl Experiment prove?

A

DNA is semiconservative

19
Q

How did the Meselson-Stahl Experiment work?

A

Parent strand was heavy, all the nucleotides added were light and all the follow up generations were weights correlated with the new strand being light.

20
Q

What prime end are nucleotides added to on a newly synthesized strand?

A

3’ end

21
Q

What is a dNTP?

A

Deoxyribonucloside triphosphate (dATP, dTTP, dCTP, dGTP)

22
Q

How many origins of replication do eukaryotes have (it’s not an actual number)? How many origins of replication do prokaryotes have?

A

Eu: Many
Pro: One