Rickettsiales Flashcards
Neorikettsia invade what type of cells
Surivive in macrophages and mononuclear cells
Anaplasma invade what types of cells
Rickettsia rickettsii is transmitted through
Ixoided and Dermacentor ticks
Rickettsiales are classified based on
Sequence comparison of 16s rRNA gene
Transmission of Coxiella burnetti through
Arthropod
Vector of Neorickettsia
Fluke
_____________________
All stages within fluke life cycle are infectious
Pathological findings of African Heartwater
Vasculitis with effusion
Epithelial/Endothelial hemorrhage
Pericardia effusion and encephalitis
Enlarged - splee, liver, lymph nodes
Bone marrow depression
Causative agent of Potomac Horse Fever
Neorickettsia risticii
Infectious Canine Cyclic Thrombocytopenia is caused by
Anaplasma platys
Cellular and humoral immune mediated response to Ehrlichia canis conribute to
Blood cell destruction
Bone marrow depression
Polyarthritis
Uveitis
Immune complex deposition
Treatment of African Heartwater
Tetracycline
Pathogenesis of Salmon Poisoning Disease
- Ingestion of contaminated fish
- Attachment and penetration of duodenal mucosa by trematode
- Hemorrhagic enteritis with bacterial spread to lymph node, spleen, liver, lungs, brain and thymus
Clinical signs of Bovine Anaplasmosis
Subclinica vs peracute
Depression, anorexia, fever, anemia, icterus
Pathological lesions of Aegyptianellosis
Hepatosplenomegaly and punctiform hemorrhages of serosal surface
Causative agent of Canine Granulocytic Ehrlichiosis
Ehrlichia ewingii
Causative agent of Aegyptianellosis
Aegyptianella pullorum
Neorickettsia
Coccoid
Intracytoplasmic
Found within vacuoles of monocytes, macrophages and enterocytes
Gram Negative
Anaplasma
Coccoid - ellipsoid bacteria
Cause anemia, thrombocytopenia and leukopenia
Stain blue/purple with Wright-Giemsa
Phase 1 antigenic phase of Coxiella burnetti is indicative of what type of infection
Chronic Infection
Causative agent of Equine and Canine Granulocytic Anaplasmosis
Anaplasma phagocytophilum
Treatment of Ricketssia rickettsii
Chloramphenicol, Fluoroquinolones and Tetracyclines
Supportive Therapy
Causative agent of Human Monocytic Ehrlichiosis
Ehrlichia chaffeensis
Diagnosis of Anaplasma platys is by
Blood Smear - Giemsa
Neorickettsia helminthoeca infects what type of tissues
Lymphoreticular tissues
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Lymph nodes, peyers patches
Ehrlichia ruminatum replicates in what type of cells
Reticuloendothelial
Ehrlichia invade what type of cells
Survivve in phagocytic cells
Vector of Neorickettsia risticii
Trematode

Ehrlichia chaggeensis or Ehrlichia ewingii
Diagnosis of Potomac Horse Fever
History
Diagnosis of Coxiella burnetti
Tissue Stain - Gimenez, Giemsa, Modified Ziehl Neelson
Pathogenesis of Bovine Anaplasmosis
Transmission of anaplasma marginale is through
Hard Ticks
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Boophilus, Dermacentro, Ixodes, Rhipicephalus
Vector of Ehrlichia ewingii
Ambyloma Americanum - Lone Star Tick
Diagnosis of Rickettsia rickettsii
Culture - Yolk Sac or Cell Culture
Vector of Ehrlichia Canis
Rhipicephalus sanguineus - Brown Dog Tick
Cause

Anaplasma marginale
Bovine Anaplasmosis
Diagnosis of Bovine Anaplasmosis
Diagnosis of Anaplasma phagocytophilum by
Blood Smear - Giemsa or Wrights
Target cells of Neorickettsia helminthoeca
Canine mononuclear cells
Phase 2 antigenic phase of Coxiella burnetti is indicative of what type of infection
Acute Infection
Causative agent of Salmon Poisoning Disease
Neorickettsia helminthoeca
Vector of Ehrlichia chaffeensis
Dermacentro variabilis - American Dog Tick
Treatment of Bovine Anaplasmosis
Tetracyclines
Transmit what organism

Rickettsia rickettsii
Bovine Anaplasmosis causative agent
Anaplasma marginale
Causative agent of Rocky Mounthain Fever
Rickettsia rickettsii
Neorickettsia risticii has an affinity for
blood monocytes, tissue macrophages, and intestinal epithelial cells
Clinical signs of Salmon Poisoning Disease
Fever, vomiting, weightloss, hemorrhagic diarrhea and lymphadenopathy
Associated with what organism of Ehrlichia

Ehrlichia ruminantium
African Heartwater
Causative agent of Human Granulocytic Anaplasmosis
Anaplasma phagocytophilum
Clinical signs of chronic Ehrlichia canis infection
Dyspnea
Enlarged - spleen, lymph nodes, and liver
Polyarthritis
CNS disturbances
Pulmonary infiltration
Clinical signs of Rock Mountain Spotted Fever
High fever, anorexia, vomiting, diarrhea, petechiae or ecchymotic MM, edema
Thrombocytopenia and leukopenia
Stains to use for Neorikettsia
Macchiavello
Giemsa
Diagnosis of Salmon Poisoning Disease
Fecal - detection of Fluke eggs
Rickettsiae
Gram Negative
Non Motile - actin hijack inside cells
Clinical signs of Potomac Horse Fever
Acute, watery diarrhea
Fever
Mild colic
Laminitis
Leukopenia
Pathogenesis of Ehrlichia ruminatum
- Inoculation of the bacteria by tick bite
- Multiplication in cells lining the sinusoids of lymph nodes
- Vascular dissemination and colonization of endothelial cells
Anaplasma phagocytophilum is transmitted through
Ixodes sp ticks
Clinical signs of Aegyptianellosis
Ruffled feathers, anorexia, diarrhea, anemia, hyperthermia
Diagnosis of African Heartwater
Giemsa stained smears
Associated with what organism

Ehrlichia ruminantium
African Heartwater
Clinical signs of peracute African Heartwater
Fever
Collapse and death under convulsion
Clinical signs of Infectious Canine Cyclic Thrombocytopenia
Treatment of Aegyptianellosis
Tetracyclines
Clinical signs of acute infection of Ehrlichia canis
Fever, weight loss, pale MM, lymphadenopthy, epistaxis, thrombocytopenia, leukopenia and anemia
Antigenic variablity of Anaplasma due to
Major Surface Protein (MSP2)
Anaplasma platys is transmitted through
Rhipicephalus and Dermacenter sp ticks
Diagnosis of Ehrlichia canis
Buffy Coat Smear - Giemsa
Transmit what organism

Ehrlichia ruminantium
African heartwater
Vector of Ehrlichia ruminatum
Ambylomma
Pathological changes seen with Coxiella burnetti
Vasculitis, placentitis, splenomegaly, mild hepatitis
Q Fever of Humans is caused by
Coxiella burnetti
Causative agent of Canine Monocytic Ehrlichiosis
Ehrlichia canis
Coxiella burnetti
Survives in environment
Can be disemminated by aerosol route
Bio weapon
Reportable
Causative agent of African Heartwater
Ehrlichia ruminatum
Clinical signs of acute african heartwater
Fever
Hyperexcitability
Muscle tremors
Ataxia
Defect in C.P
Head pressing
Coma
Seizures
Vector or Aegyptianella pullorum
Tick
Pathogenesis of Coxiella burnetti
- Inhalation/Ingestion/Arthropod Bite
- Gain access to vascular endothelium, renal and respiratory epithelium
- Multiply within phagosome
- Mostly asymptomatic disease
Treatment of Ehrlichia chaffeensis and ewingii
Tetracyclines and Imidocarb Dipropionate
Transmission of what organism

Anaplasma marginale
Organism

Anaplasma phagocytophilum
Ehrlichiae is an obligate intracellular bacteria of what type of cells
White blood cells
Pathogenesis of Ehrlichia canis infection
- Inoculation by tick bite
- Incubation period - agent proliferates by binary fission within monocytes
- Severe acute disease can develop and then progression to chornic disease