Diagnostic Bacteriology

Question 1

Presumptive Diagnosis

Answer 1

Diagnosis based on history, symptoms and direct examination of clinical samples

Question 2

MALDI-TOF

Answer 2

Biochemical identification method, measures unique molecular fingerprint of an organism

Question 3

Identifying the host immune response through

Answer 3

Humoral immune response - antibodies

Cell mediated immune response

Question 4

Sensitivity

Answer 4

Percentage of true positives in a sample population that are correctly identified as positive by a test

Question 5

_____________ provides immediate information on the number, morphologic characteristic, and gram staining properties of microorganisms and host cellular response.

Answer 5

Direct microscopic examination provides immediate information on the number, morphologic characteristic, and gram staining properties of microorganisms and host cellular response.

Question 6

Antibody Titer

Answer 6

Highest dilution of the serum sample which will give a positive result in a particular test.

Question 7

What is a limitation of PCR identification?

Answer 7

You have to know what you are looking for

Question 8

Identify the agent by

Answer 8

Direct detection of agent

Isolation and identification

Direct detection of antigens/toxins

Nucleic acid detection

Question 9

Measurement of the antibody titer usually expressed as the ____________ of the (highest/lowest) dilution of serum that positively reacts in a specific test.

Answer 9

Measurement of the antibody titer usually expressed as the reciprocal of the (highest/lowest) dilution of serum that positively reacts in a specific test.

Question 10

False Positives can be due to

Answer 10

Prior antigen exposure, vaccination, or cross-reactions

Question 11

Positive Predictive Value

Answer 11

Percentage of all positive test results that are true positives

Question 12

2 ways to identify infectious causes

Answer 12

Identification of the agent

Identification of the host immune response

Question 13

Negative Predictive Value

Question 14

Culture results typically take ________ to obtain.

Answer 14

Culture results typically take 24-72 hours to obtain from laboratory.

Question 15

Because of the time it takes to receive culture results it is important for the veterinarian to know:

Answer 15

Specific pathogens involved in clinical syndromes and antimicrobial susceptibility patterns of common pathogens

Question 16

Fluids in which you can collect specimen

Answer 16

Urine

Transudates, Exudates, Pus and Discharge

Lavage or Wash

Feces

Blood

Question 17

Ideal test has (high/low) sensitivity and (high/low) specificity.

Answer 17

Ideal test has (high/low) sensitivity and (high/low) specificity.

Question 18

Seroconversion

Answer 18

Antibody development after exposure to a pathogen or antigen

Question 19

Examples of differential stains

Answer 19

Gram Stain

Acid Fast Stain

Wright/ Giemsa

Question 20

Specificity

Answer 20

Percentage of true negatives in a sample population that are correctly identified as negative by a test

__________________

Accuracy of the test

Question 21

PCR

Answer 21

Non culture based from clinical samples, identifies isolates

Question 22

False Negatives can occur due to

Answer 22

Anergy or immunosuppression

Question 23

Single most important and cost effective laboratory procedure is:

Answer 23

Direct Microscopic Examination

Question 24

When collecting urine, ____________ is preferred over ______________.

Answer 24

When collecting urine, cystocentesis is preferred over catheterization.