Gram Positive Rods Flashcards
Listeria ___________ causes occastional infection and is associated with ruminant abortion.
Listeria ivanovii causes occastional infection and is associated with ruminant abortion.
___________ are large, endospore forming, rapidly growing bacteria that are important soil saphrophytes.
Bacillus spp. are large, endospore forming, rapidly growing bacteria that are important soil saphrophytes.
Trueperella ________ can cause summer mastitis
Trueperella pyogenes can cause summer mastitis
Facultative intracellular pathogen, lipid-rich cell envelope with mycolic acid capable of intra-macrophage survival and granuloma formation
Rhodoccocus equi
The capsule of Bacillus anthracis is unique because
The capsule of Bacillus anthracis is unique because it is composed of polymers of D glutamic acid
Edema Factor
Component of Anthrax toxin - inhibits neutrophil function
Bacillus anthracis has resistance or reduced susceptibility to
Trimethoprim/sulfa
Erythromycin
Azithromycin
Virulence factors of Corynebacterium pseudotuberculosis
Phospholipase D
Lipid Coat
Main species of Nocardia
N. asteroides
N. brasiliensis
N. otitidiscavarum
Bacillus ___________ is an insect pathogen.
Bacillus thuringiensis is an insect pathogen.
_________ are the most important reservoir for Erysipelothrix spp.
Pigs are the most important reservoir for Erysipelothrix spp.
Internal form of caseous lymphadenitis
Characterized by abscess formation within internal organs
________________________
More common in sheep
Commensal organism in prepucial mucosa of boars, sexually transmitted and causes porcine cystitis and pyelonephritis
Actinobaculum suis
Diagnosis of B. anthracis
Blood smear
Aerobic Culture
PCR
Describe the appearance of Rhodococcus equi bacterial colonies
Mucoid with a salmon pink color
The presence of mycolic acid is important because
The presence of mycolic acid is important because acid-fast stain can be used for diagnosis
Treatment of Nocardia
Aminoglycoside + Carbapenem
Trimethoprim/Sulfa
Diagnosis of Rhodococcus equi
CBC/ Serum Chemistry
Thoracic Radiographs
Culture
PCR
Reportable Bacillus Pathogen
Bacillus Anthracis
Erysipelothrix __________ is less common, it is avirulent in pigs but associated with disease in dogs.
Erysipelothrix tonsillarium is less common, it is avirulent in pigs but associated with disease in dogs.

Corynebacterium diphtheriae
Causes lumpy jaw in cattle
Actinomyces bovis
Diagnosis for Corynebacterium pseudotuberculosis
Symptoms
Abscess/ Chronic Weight Loss
Culture
Clinical Signs:
Neurological symptoms
Unilateral trigeminal and facial nerve paralysis
Purulent endophthalmitis
Listeria spp
Erysipelothrix has an intrinsic resistance to
Vancomycin
Aminoglycosides
Sulfonamides

Listeria spp
Causes Rain Scald
Dermatophilus congolensis
Important virulence factors of B. anthracis
Capsule
Anthrax toxin
________________________
Both required for virulence
Pleomorphic, faculative intracellular bacterium. In gram stained smears characteristic long or branching filaments and a tendency to fragment into rods and cocci
Nocardia
Common secondary invader in shipping fever pneumonia in cattle
Trueperella pyogenes

Dermatophilus congolensis
Cause

Erysipelothrix rhusiopathiae
Treatment of Dermatophilus congolensis
Penicillin G and Tetracycline
Cause human childhood disease diphtheria
Corynebacterium diphtheriae
Diagnosis of Listera sp
Antemortem - Presumptive Dx and History
Histopathology - Microabscesses in the brain
Bovine Farcy
Cutaneous nocardiosis in cattle caused by Nocardia asteroides or mycobacteria lesions, typically associated with lymphangitis and lymphadenitis
External form of caseous lymphadenitis
Characterized by abscess formation in or near major peripheral lymph nodes
___________________
More common in goats
Protective Factor
Component of Anthrax Toxin - cell binding factor responsible for translocation into the cell
The vaccine for B. anthracis must contain what components of the bacteria?
Anthrax Toxin
Chromosome
Bacillus _________ are aerobic, non motile, large with squared ends arranged in chains.
Bacillus anthracis are aerobic, non motile, large with squared ends arranged in chains.
Causes Murine pseudotuberculosis
Corynebacterium kutscheri
The only symptome of per acute septicemia caused by B. anthracis in ruminants
Death
Cause and Disease

Corynebacterium pseudotuberculosis
Caseous lymphadenitis
Summer mastitis
communicable disease among pastured daily cattle during the dry period
Bacteria frequently associated with pyogenic infections in livestock
Actinomyces
Actinobaculum
Trueperella
____________ outbreaks typically occur due to feeding poor quality soilage.
Listera spp outbreaks typically occur due to feeding poor quality soilage.
The most common way animals get exposed to B. anthracis
Exposure to endospores
Causes caseous lymphadenitis in sheep and goats
Corynebacterium pseudotuberculosis
Causes pyogranulomatous osteomyelitis
Actinomyces spp
Species of Corynebacterium that may harbor bacteriophage containing diptheria toxin
C. pseudotuberculosis
C. ulcerans
____________ is the most common presentation of Listera infection in monogastrics.
Septicemia is the most common presentation of Listera infection in monogastrics.

Bacillus anthracis
Treatment for Erysipelothrix spp
Penicillin
Tetracycline
Lincomycin
Tylosin
Act A virulence factor of _______________ allows intracellular movement to adjacent cells through bacterially induced host actin polymerization.
Act A virulence factor of Listeria monocytogenes allows intracellular movement to adjacent cells through bacterially induced host actin polymerization.
Cause and Disease

Dermatophilus congolensis
“Rain Scald”
Cause

Actinomyces hordeovulneris
__________ is the most common presentation of Listeria infection in ruminants.
Encephalitis is the most common presentation of Listeria infection in ruminants.
McFadyean Reaction
Diagnostic tool for B. anthracis, stains the capsule as a pink shadow with polychromatic methylene blue
Species of Nocardia associated with abortion in horses, cattle, sheep and swine often with no other signs in the dam.
Nocardia asteroides
__________ are pleomorphic, non spore forming and non motile. They contain mesodiaminopimelic acid and short chain mycolic acid in their cell wall.
Corynebacterium spp are pleomorphic, non spore forming and non motile. They contain mesodiaminopimelic acid and short chain mycolic acid in their cell wall.
Cause and Disease

Corynebacterium kutscheri
Murine pseudotuberculosis
Nocardia species most commonly isolated in dogs and cats.
Nocardia asteroides
Koch’s postulates were developed using
Koch’s postulates were developed using Bacillus anthracis
Bacillus _________ is an obligate mammalian pathogen.
Bacillus anthracis is an obligate mammalian pathogen.
Zoospores attacted to moist damaged skin, cause epidermal abscesses with hyperkeratosis.
Dermatophilus congolensis
Previous names of this bacteria were Corynebacterium, Actinomyces and Arcanobacterium pyogenes
Trueperella pyogenes
Vaccination available for Erysipelothrix spp for
Pigs and Turkeys
Causes Contagious Bovine Pyelonephritis
Corynebacterium renale group
Diagnosis of Dermatophilus congolensis
Microscopic examination of scabs
Culture
PCR
Bacillus ________ is a rare cause of opportunistic infections and food poisoning in mammals.
Bacillus cereus is a rare cause of opportunistic infections and food poisoning in mammals.
Rhodococcus equi causes most serious cases of pneumonia in ________ old foals.
Rhodococcus equi causes most serious cases of pneumonia in 1 to 4 month old foals.

Corynebacterium bovis
Treatment of Corynebacterium pseudotuberculosis
Not considered “curable”
Lancing and Draining/ Surgical excision of abscesses
Extra label use- Penicillin, Rifampin, Tulathromycin
Cause of Foal Pneumonia
Rhodococcus equi
Actinomyces ___________ can be transmitted to animals through fox tail grass awns to cause abscess and epicarditis (chronic)
Actinomyces hordeovulneris can be transmitted to animals through fox tail grass awns to cause abscess and epicarditis (chronic)
In horses, Corynebacterium ____________ causes ulcerative lymphangitis of lower extremities and abscesses in the pectoral region and ventral abdomen.
In horses, Corynebacterium pseudotuberculosis causes ulcerative lymphangitis of lower extremities and abscesses in the pectoral region and ventral abdomen
Diagnose Actinomyces spp by
Culture - Must request aerobic and anerobic
Microscopic examination
Causes dematitis and hyperkeratosis in mice
Corynebacterium bovis
Treatment of Rhodococcus equi
Macrolide + Rifampin
Listeria monocytogenes Treatment
Penicillin
Ceftiofur
Tetracycline
Erythromycin
Trimethoprim/Sulfa
Ampicilling + Genamicin
Virulence factors of Rhodococcus equi
Capsule
Viruclence Associated Proteins
Mycolic Acid/Techoic Acid
Cholesterol Oxidase
Phospholipase C
Synergistic hemolysin inhibition (SHI) Test
Detects antibodies to the phospholipase D exotoxin

Actinomyces spp
Erysipelothrix _________ is most frequently isolated, most common in pigs and birds.
Erysipelothrix rhsiopathiae is most frequently isolated, most common in pigs and birds.
Camp test
Cholesterol oxidase enhances hemolysis of weakly hemolytic organisms

Trueperella pyogenes
Treatment for Bacillus anthracis
Penicillin
Tetracycline
Doxycycline
Ciprofloxacin
Predisposing factors that increase exposure of animals to anthrax spores
History of previous deaths
Flooding
Alkaline soil conditions
Warm Temperature
What is the staining technique? What is it used for?

McFadyean Stain
Bacillus anthracis
Disease and Cause

Caseous lymphadenitis
Corynebacterium pseudotuberculosis
Cause and Disease

Corynebacterium renale group
Contagious bovine pylenephritis
Colonies vary, but usually small, slow growing and “molar tooth like”
Actinomyces spp
Cause and Disease

Actinomyces bovis
Lumpy Jaw
Diphtheria toxin is carried by a
Diphtheria toxin is carried by a bacteriophage

Nocardia
Diagnosis of Erysipelothrix spp
Culture
PCR
Corynebacterium renale group consists of
C. renale
C. cystiditis
C. pilosum
Listeria _____________ is associated with disease in multiple hosts, including humans.
Listeria monocyogenes is associated with disease in multiple hosts, including humans.
Divides in two planes to give “tram track” appearance and produce coccoid fragments which become motile zoospores
Dermatophilus congolensis
Swine from ____________ of age and pregnant sows are most susceptible to Erysipelothrix spp.
Swine from 3m to 1y of age and pregnant sows are most susceptible to Erysipelothrix spp.
Diagnosis of Nocardia
Culture
Acid Fast Stain
Histopathology
Habitat of Rhodococcus equi
Soil, however virulence is maintained in horses
Non-hemolytic, medusa head colonies
Bacillus anthracis
Lethal Factor
Component of Anthrax Toxin - induces cell death and hypoxia induced tissue injury/shock
Causes Pigion Fever - Colorado Strangles - Dry-Land Distemper
Corynebacterium pseudotuberculosis
Treatment of Acitomyces sp infection
Penicillin G - long term and high doses
Surgical excision of foreign bodies
What season do Listeria spp outbreaks typically occur?
Winter, Early Spring

Erysipelothrix spp
Most species of __________ recovered from animals are not considered pathogenic.
Most species of Corynebacterium recovered from animals are not considered pathogenic.
Three components of Anthrax Toxin
Edema Factor
Lethal Factor
Protective Factor
__________ is isolated from many species of animals and is found ubiquitously in the environment as it is capable of growing at a wide range of temperatures
Listeria spp is isolated from many species of animals and is found ubiquitously in the environment as it is capable of growing at a wide range of temperatures

Bacillus spp