Enterobacteriaceae Flashcards
Virulence factors of VTEC
F18 - adhesion small bowel
Exotoxin - VT2e or Stx2e
Endotoxin - acute mortality
Predisposing factors of E coli UTI
Hygiene
Water intake
Obstipation
Age
Individual differences
How do you prevent septicemia caused by E. coli in bones?
Colostrum
General characteristics of heat stable enterotoxins in pigs
Low molecular weight
Little antigenic
Withstand 15 minutes at 121C
Bio/serogroup of RPEC that is pathogenic to suckling rabbits
1+ / O109
Pathogenesis of septicemia causing E coli in bovines
- Oral uptake
- Lympoid tissue
- Septicemia
- Umbilical cord
- Septicemia
F17 a and b virulence factors of ETEC in bovines are frequently associated with
CNF2
Diagnosis of EPEC
Isolation and PCR
F5 (K99) Adhesion Factor
Mannose resistant
Expression is temperature dependent
Only in very young animals
In pigs and bovine
A break in the equilibrium between maternal immunity and infection pressure causes ETEC disease in pigs, what can cause this break?
Increased infection pressure
Decreased maternal immunity
Appearance of a new type
Diagnosis of VTEC
Clinical signs
Pathological findings
Bacteriology - fecal sample
Demonstration of virulence factors - PCR
Coliform enumeration is performed to evaluate
Fecal/Sewage contamination
Enterotoxigenic (ETEC) adhesion factors in the small intestine in pigs
F4 (K88)
F5 (K99)
F6 (987P)
F18
F41
AIDA-I - Non Fimbrial Adhesion
CS31A
Related to F4
Frequency in relation to diarrhea in older animals
Pathogenesis of ETEC in bovines
- Oral uptake
- Multiplication
- Enterotoxin production
- Secondary septicaemiae
Important E. coli pathotypes in pigs
ETEC
VTEC
EPEC
UTI
MMA
Enteric pathotypes of E. coli
ETEC - Enterotoxigenic
EHEC- Enterohemorrhagic
VTEC/STEC - Verotoxigenic/ Shig-toxin like
EPEC - Enteropathogenic
EAEC - Enteroadherent
DAEC - Diffuse Adherent
EIEC - Enteroinvasive
EAggEC - Enteroadherent Aggregative
Most common fimbrial virulence factor associated with ETEC in bovines
F5
Diagnosis of RPEC
Clinical symptoms
Isolation and subtyping
Pathogenesis of Neonatal colibacillosis
- Contamination of egg shell
- Upon cooling after lay - shrinking of content and entrance of E. coli
- Yolk rest infection
- Sepsis → death
- Polyserositis, Airsac, Pericardia
- Growth retardation
- Neonatal contamination or Manipulation of chicks
- Yolk rest infection
- Sepsis → death
- Polyserositis, Airsac, Pericardia
- Growth retardation
- Yolk rest infection
Extracellular virulence factors of E. coli
Enterotoxins
Cytotoxins
Siderophores
STa enterotoxn
Activation of guanylate cyclase system
Na absorption
Cl and HCO3 secretion
Pathogenesis of ETEC in pigs
- Ascending from lower bowel
- Oral uptake
- Small intestine
- Colonization
Treatment of ETEC in pigs
Hydration - electrolyte solution plus glucose and amino acids
Antimicrobials
Septicaemiae caused by E. coli is usually (primary/secondary)
Secondary
F41 Adhesion Factor
Chromosomal
Mannose resistant
Frequently together with F5
Rare
Pigs and Bovines
Characteristics of neonatal ETEC in pigs
F5, F6, F41
STa STb
Non hemolytic
Pathogenesis of Respiratory Colibacillosis
- Excretion via feces
- Increase in infection pressure
- Inhalation of pathogenic E. coli
Pathogenicity of Escherichia coli
Strain dependent
Facultative pathogenic
Obligate pathogenic but age dependent
Enteric E coli diseases that effect dogs
ETEC
EPEC
VTEC
EIEC
Benefits of Enterobacteria
Microbial antagonism
Breakdown and absorption of food
Waste processing
Vitamin K production
EPEC
Rare
Diarrhea at weaning
Attaching and effacing
T3SS and injection of effector proteins
Virulence factors of E. coli causing sep
Seru resitance
Iron uptake systems
Endotoxin
Capsule
Adhesion factors - P, F17, AfaE-VIII, CS31A
CNF1 CNF2 toxins
Treatment of RPEC of rabbits
Antibiotics
Diagnosis of ETEC of bovines
ELISA
Culture + Viruclence Factors - agglutination or PCR
Diagnosis of ETEC in pigs
Detection of virulence factors with agglutination tests or PCR
Enterobacteria are gram (positive/negative)
Negative
MMA stands for
Metritis - Mastitis - Agalactiae
Cell associated virulence factors of E. coli
Endotoxin
Capsule
Fimbrial Adhesions
Non-Fimbrial Adhesions
Characteristics of EPEC and EHEC in bovines
1 - 8 weeks of age
Mucoid diarrhea +/- blood
Large intestine
Treat with antibiotics
Forms of E coli infection in dogs and cats
Enteric form
Cystitis and pyometra