RICKETTSIA CHLAMYDIA MYCOPLASMA

Question 1

what are obligate intracellular parasite

Answer 1

parasite that can be reproduce only in living cell ex :rickettsia, chlamydia

Question 2

order-rickettsiales family-rickettsiacea of ricket is transmitted thru

Answer 2

arthropod vectors :flea,louse(feces) tick,mite(bite)

Question 3

pathogenesis of ricketsia

Answer 3

disseminate thru bloodstream
endothelial cell induce phagocytosis
escape from phagosome
multiply intracelullarly within cytoplasm
destroy host cells

Question 4

clinical manifestation of rickettsia

Answer 4

rash :capilary leak to interstitial spaces causes vasculitis necrosis of blood vessels wall
fever :edema,hypovolemia,hypotension and adult respiratory distress syndrome

Question 5

family -anaplasmatacae genus-erlichia,anaplasm replicate at _____

Answer 5

phagosome of WBC cause systemic disease

Question 6

3 laboratory diagnosis :

Answer 6

1- serological methods -immunofluoresence, enzyme immuno-assay, immunoperoxidase
2- isolation of the organism -embryonated eggs
3- detection of organism in tissue -immunofluoresence, immunoenzyme methods to detect antibody

Question 7

ricketsia disease can be treated by

Answer 7

tetracyclines, doxycycline, chloramphenicol

Question 8

chlamydia trachomatis affect humans
chlamydophila pneumonia affect humans, animals
chlamydophila psitacci - zoonotic infection

Answer 8

species

Question 9

chlamydiae components

Answer 9

DNA RNA ribosomes

Question 10

extracelullar (outside) and inactive form of chlamydiae

Answer 10

elementary body

Question 11

intracelullar, not infecious and active form of chlamydiae

Answer 11

reticulate body

Question 12

site of infections for chlamydiae

Answer 12

epithelial layer -ocular,genital,pulmonary mucosa

Question 13

virulence mechanism of chlamydiae

Answer 13

prevents fusion of phagosome with lysosome

intracelullar replication -kill all cells

Question 14

type of diseases of chlamydiae

Answer 14

chlamydia trochomatis -blindness, genital ulcer
chlamydophila pneumoniae -atypical pneumonia
chlamydophila psitacci -parrot fever, zoonotic pneumonia from avian strains

Question 15

servours ABC common diseases

Answer 15

trachoma

chronic follicular conjuctivitis > corneal scarring > blindness

Question 16

servours D-K common diseases

Answer 16

oculogenital disease - opthalmia neonatrum (infantile conjuctivitis)
non gonococcal urethritis(NGU) -purulent urethral discharge STD

Question 17

servours L123 common diseases

Answer 17

lymphogranuloma venereum - STD begins w genital ulcer cause strictures in urinary tract

Question 18

3 laboratory diagnosis

Answer 18

cell culture- maccoy cells w cycloheximide
antigen detection- detect inclusion body by enzyme immunoassay
serology -detect specific antibody

Question 19

glemsa stain is used for _____

Answer 19

elementary body

Question 20

iodine stain is used for _____

Answer 20

inclusion body

Question 21

erythromycin antibiotic is used for ____

Answer 21

babies

Question 22

list mycoplasma species :

Answer 22

m. pneumoniae
m. hominis
m.genitalium
ureaplasma urealyticum

Question 23

characteristics of mycoplasma :

Answer 23

lack of cell wall
cell membrane contains sterol -like eukaryote
require special culture media -require animal protein(blood serum) and sterol for growth

Question 24

mycoplasma pneumoniae virulence factors are

Answer 24

adhesin protein called PI
it damage the epithelial cell
interferes w ciliary action and leads to desquamation of epithelial cells
fx as superantigen -stimulate inflammatory cells to migrate to the sie of infection and release cytokinase

Question 25

what disease causes by m hominis&u. urealyticum

Answer 25

respiratory infection disease in newborn