PRACTICAL 1

Question 1

Which of the following process is suitable to do cleaning for the workstation at the laboratory?

Answer 1

disinfection

Question 2

Which of the following chemical agents is suitable for hand washing (animate objects) when check up the patient?

Answer 2

antiseptic

Question 3

Which of the following chemical agents is the main ingredients for sanitizer:

Answer 3

ethyl alcohol and isopropyl alcohol

Question 4

Which of the following option is the mechanism of action for sanitizer agent?

Answer 4

denaturing bacterial proteins

Question 5

Which of the following reagent is not used in Gram staining procedure?

Answer 5

carbol fushion (Red)- acid fast stain

Question 6

Which of the following cause make Gram-Positive bacterial cells retain the primary stain (Crystal violet) after decolourization

Answer 6

thiccc peptidoglycan layer

Question 7

Which of the following cause make Gram-negative bacterial cells to be stained by counterstain (safranin) after decolourization

Answer 7

thin peptidoglycan layer

Question 8

Which of the following outcome result for the bacterial cells in which CV-I complex don’t come out of the cells during the decolourization

Answer 8

gram + purple violet colour

Question 9

Why Mycobacterium Tuberculosis cells are stained by Ziehl-Neelsen not Gram stain?

Answer 9

mycolic acid layer

Question 10

Why do we heat in Ziehl-Neelsen (Acid Fast) staining technique?

Answer 10

to enable the dye to penetrate thru waxy mycobacterial cell wall