Rh System C and E ags Flashcards
Anti-A Anti-B Anti-D AI cell B cell
+4 0 +4 0 +4
Interpretation?
A pos
Anti-A Anti-B Anti-D AI cell B cell
0 0 0 +4 +4
Interpretation
What do you do with 0 Anti-D
O neg
AI null gene
H/H homozygous
d/d homozygous
With the negative Anti D use AHG to confirm rxn
RhD is?
polymorphic
Another word for Partial D?
where is it located?
D-mosaic
located outside of the cell
Where is Weak D located?
Inside the cell
Describe Rh Null
causes?
Rh null regulator
Rh null amorph
Rh deficiency syndrome
Rh Null regulator: absence of RhAg regulator does not place Rh ag onto the cell.
Rh null amorph: Rh genes genetically mutated to missence genes
Symptoms of Rh null
chronic hem anemia, stomatocyte/sphero
overactive ATPase
Serology of Rh null
lack of?
reduced?
lack of Rh Ag (may or may not have rhag)
Lack LW and FY5: reduced expression of SsU
Describe C ag
apart of?
mutations?
hybrid?
antitethical ag: C or c
apart of RHCE
Mutations in caucasions C ag
RHDII-CE hybrid expresses no D ag
C frequency in
White
Black
Chinese
Indian
Hispanic
W: 70
B: 30
C: 90
I: 85
H: 70
c frequency in
White
black
chinese
indian
hispanic
who is it most likely in?
w: 80
b: 96
c: 60
i: 50
h: 40
C antibody briefly described
what kind of ig
stimulation
causes?
morphology?
IgG
immune stimulated
Anti-c is next immunogenic
can cause HDN
in pairs
Immunogenicity rankings from D
D>c>E>C>e
Describe the E ag
most common
high
E ab
more
stim?
antethetical E and e
most common mutation on e
high incidence Ag
Ab: E more immunogenic
immune stimulated
E frequency in W/B/C/I/H
e frequency
E
W: 30
B 20
C 50
I 20
H 25
e
w 100
b 100
c 90
i 100
h 80
Is e or E more frequent?
e
Anti-D Anti-C Anti-c Anti-E Anti-e
+4 +3 0 +4 +3
What ag are present on the Rbcs?
what ab can they make?
DCEe
controlls are screen cells
phenotype Rh
they can make Anti-c Ab
Describe Ficher-Race
medelian inheritence of Co-dom expressed Ag
3 genes = 3 ags closely linked
Named: DCcEe
Describe Weiner
mend. inhert. co-dom expressed Ag
3 alleles from a single locus on a gene
Named: Rh0, hr’,hr’’
Describe Tippett
2 genes on chrom 1
RHD for D and RHCE for Ce 1
Describe the translation of these names to Weiners
DCe
DcE
Dce
dce
DCe - R1
DcE - R2
Dce - R0
dce -Rz
Describe the translation of these names to Weiners
dCe
dcE
dce
dCE
dCe -r’
dcE - r’’
dce - r (most common)
dCE- ry (rare)
Describe C ag
capellini effect
When paured w/ D on same haplotype, condiminish D expression “c in trans” capellini efect
DCe/dCe
R1
Frequencies of
D
e
c
C
E
lower in which ethnicities?
D - 85 low white
e - 98
c - 80 low asian
C -70 low black
E - 30
T/F abx reactions are stronger with homozygous
true
What is the equation for # of units to test
units to test = 100 x # units needed / % compatibility
% compatibility equation
100-Ag frequency
Predict the genotype
Anti-D Anti-C Anti-c Anti-E Anti-e
+4 0 +3 +3 +3
DcE/Dce
Combo Ab
Anti-f
ce on same haplotype (compound) c-units compatible
Combo Ab
Anti-G
D or C doesnt matter which
matters for Rhogam not transfusion
Concominant Ab
Anti-c and Anti-E
Anti D
Anti -C
Anti-c + Anti-E togehter DCe common haplotype
Anti-D may have Anti-G
Anti-C is often found w/ Anti-D
Anti-F is often found w Anti-C
D–
causes
Deletion Ag
D expression is enhanced “exalted” D can make Rh-17
Causes: RHD disrupts RHCE more D expressed/transcribed
RHCE silencer (hispanics)
How many genes are in Rh
two genes plus rhag
