ABO blood Types Flashcards
What controls A1/A2 cells
anti-A1 lectin +/-
Briely describe bombay again
what gene adds….
genotype/blood type
chromosome?
gene FUT1 adds fucose type 2 chains on rbc, genotype H
O blood type
Chromosome 19
A/B
genotype
chromosome
genotype A/B
Hh/HH/hh
Chromosome 9
Blood type %
AB
A
B
O
AB 5%
A 35%
B 15%
O 50%
give examples of antithetical ag
A1 cells/B cells
if a dIscrepcency is detected before a necessary transfusion
eX
transfusion is necessary before resolution, only TYPE O will be used
this would be like if forward/reverse dont match
Hxt doesnt match
ABO ags are what Ig? what do they do
IgM, fix compliment/hemolysis
How do enhance discrepency
use what on forwards type?
use what to verify agglutination?
make it colder (5-15 min)
Use Anti A,B on forwards type
Use saline control for forward type to verify agglutination
Anti-A Anti-B A1 B cells
+4 +4 +1. 0
What is the forward/reverse?
What do you do to enhance?
Forward is AB
Backwards is B
Use A1 lectin on forwards
Use A2 cells on reverse
Anti-A Anti-B A1 B cells
4+ 4+ +1 0
A1 cell
0
what type of blood can you give?
lacks branches
A subgroup
A2B w Ab
can give B/O blood
Anti-A Anti-B A1 B cells
4+ 0 0 0
What is wrong here?
too little….
sometimes immunocomp pt cant…
too little reactivity, MOST COMMON DISCREPENCY
make rxn colder
sometimes immunocomp pt cant make Ab because of irrateated blood products
Anti-A Anti-B A1 B cells
4+ 4+ 4+ 4+
Whats wrong here?
Forwards vs reverse type?
How do you fix?
Forwards AB
Reverse O
too much reactivity
WARM SAMPLE
use saline/wash cells
test against screen cells
Red cell donor selections
A
B
O
AB
What are their donor rbcs?
A: OA (anti-B)
B: OB (anti-A)
O: O
AB: OAB (AB)
Plasma Blood Donor
A
B
O
AB
What are their plasma products?
A: AB, A
B: AB: B
O: AB, O, B , B
AB: AB
What is the universal plasma donor
AB
Universal recipient
O
85% of the population can get what plasma
Type A plasma
Describe ABO Ag
n
ig
fix
c
titer
naturally occuring
IgM
fix compliment
cold rxns
titer decreases with diseases and old age
Describe Secretions briefly
What determins secretor status fut
chromosome codes for?
dominant?
Chrom 19 codes FUT2 gene determins secretor status, Se is dominant
If carb branches outside of the cell are arranged specifically what are they?
attached to…
Type 1, , attached to soluble secretion in body fluids like saliva/tears
what does se mean
non functional secretors
20% non secretors
Describe Type 1
chains in …..conv by same….
chains in secretors are converted by same transferases as type II chains on rbc to A or B
T/F FUT1/FUT2 are more likely to be impacted since they are closer together
True
Hh/HH codes for
FUT1
SeSe/sese codes for
FUT2
Describe FUT3
chromosome?
genotype?
adds … to ….chain in …..only
on chrom 19
Le le (LeLe,leLe)
adds fucose to type I chain in different spot in secretions only
T/F all FUT are on the same chromosome
True Chrom 19
Functional FUT1/FUT2 but problem is
Leb ag (higher frequency)
Non secretors of FUT3 are called
LeA
What are examples of non anthetical genes
Lea/Leb
Briefly describe the Bombay major characteristics
Ag:
Sec:
hh, se (Unknown ABO) Le
Ag: Lea
Sec: Lea
Briefly describe parabombay characteristics (major)
Ag:
Sec:
hh, SeSe, A or B
Ag: Leb, small amount of Lea
Sec: A or B
Describe Anti-Lea
most common
most common transiently in pregnant women
“Secretor status”
inhibition test pt saliva to completly block reactivity
“Ab ID”
uses screen cells to see if Ab to Lewis Ag
