Complex Ab problems Flashcards
Test pannels in the same way you test screen cells….
recognize patterns
run autocontrol
phenotype
Abnormal results in complex ab problems
mixed field, positive autocontrol, non pattern match, weak reactivity, all cells positive
High incidence doesnt mean clinically significant
defining feature
may mask clinically significant positive reactivity
defining feature O in AC
HTLA
special test?
often?
has strong rxn in plasma
special test to diagnose: AB titer
often high incidence/variable reactivity
Low incidence
doesnt mean insignificant
example: anti-k anti-cw together
Dosage
weaker rxn w heterozygous
Non specific Ab
ab that have been ruled out clin significant, pattern doesnt match
unusual suspects
Neutralization
uses
QC: dil
Expect:
uses a substance to bind the Ab
QC: dilution control parallel test
Expect: Agglutination positive
P1
hydatid cyst fluid
Lewis
saliva
Chido/rhogers
serum
Sda
urine
I
breast milk
Adsorption
uses?
auto?
diff?
control? uses abs s against?
expect?
uses rbc to bind ab
autoabsorption
differential abosption
Control: uses abs serum against abs cell
Expect negative agglutination
Chemicals
destabilize bonds, break IgM ab/ag that are bonded to cell
Enzymes
enhance/depress/no effect by cutting amino acids and chains
Phenotyping
QC positive
QC negative
QC positive control = hetero ag +
Negative: ag neg
False pos in phenotyping
false neg
false pos: transfused cells/pos DAT
False neg: partical/hetero expression Anti-sera
Selected panel cells
of cells needed depends on how many ab are left not ruled out
confirm/elim Ab ID
ID new AB when previous known ab are present
