RETICULOCYTE LAB Flashcards
Reticulocytes in peripheral blood
1 day
BM:
2-3 days
measures of the erythropoietic activity in the BM
reticulocyte
WHY DO WE NEED TO PERFORM RETICULOCYTE COUNT?
To check the BM ability to increase RBC production in response to anemia.
ribosome
last organelle that will be stained
supravital stains
brilliant cresyl blue
new methylene blue
supravital stains
brilliant cresyl blue
new methylene blue
T/F methylene blue will be filtered daily or before use?
true
Last stage of immature RBC
RETICULOCYTE
Cytoplasmic inclusions coprecipitate with
mitochondria
ferritin
a dye that stain living cells and its inclusions.
Supravital stain
Sample:
-whole blood ( mixed with any
anticoagulant) : adult
-Capillary Blood: for newborn
The tube is gently mixed and is left stand for 10-15 minutes
10-15 minutes
Objective used to focuse
10x objective
Objective used to focuse
10x objective
Count reticulated and non-reticulated cells.
1000
Formula for uncorrected retic ct
UNCORRECTED RETIC CT.= NO. OF RETICULOCYTE/ NO. OF CELLS COUNTED X 100
disk inserted into the eyepiece
MILLER OCULAR DISC
Permits a less labor-intensive surveying of RBC’s
MILLER OCULAR DISC
A- LARGER SQUARE
for counting reticulocytes
B- smaller square
for counting RBC counting
Mature RBCs:
light to medium green without granules
Mature RBCs:
light to medium green without granules
Reticulocytes:
light green with granules that stain deep blue
Reticulocytes:
light green with granules that stain deep blue
% Reticulocyte = No. of reticulocytes% 1000 RBCx 100
PROCEDURE:
Dry Preparation
Normal value
0.5%-2.5% (ADULTS)
4.0%-6.0% (NEWBORN)
INCREASED:
*Blood Loss
*Crisis associated with hemolytic anmeia
*Subsequent tx. for pernicious anemia, folate deficience or iron deficiency
DECREASED:
*Aplastic anemia
*Aplastic crises of hemolytic anemia
*chemotherapy or radiation
*Pernicious anemia
*Decreased erythropoiesis
results should agree within
20% more or less
