RETICULOCYTE LAB

Question 1

Reticulocytes in peripheral blood

Answer 1

1 day

Question 2

BM:

Answer 2

2-3 days

Question 3

measures of the erythropoietic activity in the BM

Answer 3

reticulocyte

Question 4

WHY DO WE NEED TO PERFORM RETICULOCYTE COUNT?

Answer 4

To check the BM ability to increase RBC production in response to anemia.

Question 5

ribosome

Answer 5

last organelle that will be stained

Question 6

supravital stains

Answer 6

brilliant cresyl blue
new methylene blue

Question 7

supravital stains

Answer 7

brilliant cresyl blue
new methylene blue

Question 8

T/F methylene blue will be filtered daily or before use?

Answer 8

true

Question 9

Last stage of immature RBC

Answer 9

RETICULOCYTE

Question 10

Cytoplasmic inclusions coprecipitate with

Answer 10

mitochondria
ferritin

Question 11

a dye that stain living cells and its inclusions.

Answer 11

Supravital stain

Question 12

Sample:

Answer 12

-whole blood ( mixed with any
anticoagulant) : adult
-Capillary Blood: for newborn

Question 13

The tube is gently mixed and is left stand for 10-15 minutes

Answer 13

10-15 minutes

Question 14

Objective used to focuse

Answer 14

10x objective

Question 15

Objective used to focuse

Answer 15

10x objective

Question 16

Count reticulated and non-reticulated cells.

Answer 16

1000

Question 17

Formula for uncorrected retic ct

Answer 17

UNCORRECTED RETIC CT.= NO. OF RETICULOCYTE/ NO. OF CELLS COUNTED X 100

Question 18

disk inserted into the eyepiece

Answer 18

MILLER OCULAR DISC

Question 19

Permits a less labor-intensive surveying of RBC’s

Answer 19

MILLER OCULAR DISC

Question 20

A- LARGER SQUARE

Answer 20

for counting reticulocytes

Question 21

B- smaller square

Answer 21

for counting RBC counting

Question 22

Mature RBCs:

Answer 22

light to medium green without granules

Question 23

Mature RBCs:

Answer 23

light to medium green without granules

Question 24

Reticulocytes:

Answer 24

light green with granules that stain deep blue

Question 25

Reticulocytes:

Answer 25

light green with granules that stain deep blue

Answer 26

% Reticulocyte = No. of reticulocytes% 1000 RBCx 100

Question 27

PROCEDURE:

Answer 27

Dry Preparation

Question 28

Normal value

Answer 28

0.5%-2.5% (ADULTS)
4.0%-6.0% (NEWBORN)

Question 29

INCREASED:

Answer 29

*Blood Loss
*Crisis associated with hemolytic anmeia
*Subsequent tx. for pernicious anemia, folate deficience or iron deficiency

Question 30

DECREASED:

Answer 30

*Aplastic anemia
*Aplastic crises of hemolytic anemia
*chemotherapy or radiation
*Pernicious anemia
*Decreased erythropoiesis

Question 31

results should agree within

Answer 31

20% more or less