activity 2 LABORATORY

Question 1

to confirm if patient is suffering from thrombocytopenia

Answer 1

direct platelet count

Question 2

MPV

Answer 2

2-20 fL

Question 3

this is where the morphology of the cell can be observed.

Answer 3

smear

Question 4

Light microscopy- Tocantins method uses?

Answer 4

uses rees and ecker fluid

Question 5

Phase-contrast microscopy: Brecker-Cronkite Method uses what reagent?

Answer 5

uses 1% Ammonium oxalate

Question 6

uses Hema analyzer or Hematology analyzer

Answer 6

automated

Question 7

EDTA BLOOD

Answer 7

• Stable for 5 hours at 20 degree celcius
• Stable for 24 hours at 4 degree celcius

Question 8

device used for counting cells

Answer 8

HEMACYTOMETER

Question 9

Count platelets in 4 lARge W square

Answer 9

LIGHT MICROSCOPY

Question 10

Count platelets in 10 RBC/small

Answer 10

PHASE CONTRAST MICROSCOPY

Question 11

read in 4 quadrants

Answer 11

TOCANTINS

Question 12

serves as indicator if the chamber is successfully filled

Answer 12

COVER SLIP

Question 13

moist chamber (incubate) for how many minutes

Answer 13

15 minutes

Question 14

to allow platelet to settle

Answer 14

incubation

Question 15

what happens if the chamber is not incubated

Answer 15

1. drying
2. not settle

Question 16

platelets have a 2-4 um diameter.

Answer 16

Phase Contrast Microscopy or Brecker-Cronkite method

Question 17

allow the platelets to be distinguished from debris which is often refractile.

Answer 17

purple sheen

Question 18

plt are smaller 1/10th the size of the RBC , round , oval , and elongated particles with light blue stain.

Answer 18

Tocantins method or Rees and Ecker Fluid-

Question 19

REESE ECKER FLUID composition

Answer 19

1. Sodium Citrate
2. Formalin
3. Brilliant Cresyl Blue

Question 20

what is the anticoagulant in the composition of reese and ecker fluid

Answer 20

Sodium citrate

Question 21

what is the preservatine in the composition of reese and ecker fluid

Answer 21

formalin

Question 22

what is the staining reagent in the composition of reese and ecker fluid

Answer 22

Brilliant Cresyl Blue

Question 23

BRECKER-CRONKITE composition

Answer 23

1% Ammonium Oxalate

Question 24

ensures background clearing by hemolizing the red blood cells

Answer 24

1% Ammonium Oxalate

Question 25

counting chamber of brecker cronkite method

Answer 25

spencer-Briteline

Question 26

IDEAL DILUTING FLUID

Answer 26

1. Should prevent platelet from adhering to the glass surface - because it might dry and might not be counted 2. Should prevent platelet from aggregating 3. Should not promote premature platelet hemolysis 4. Should make platelets readily visible under microscope

Question 27

K3 EDTA (.22%) > NH4 OXALATE (.44%) + Crystal violet (.75%)

Answer 27

UNOPETTE METHOD

Question 28

FONIO’S METHOD - uses 14% Magnesium sulfate as diluent

Answer 28

INDIRECT METHOD

Question 29

SOURCES OF ERRORS:

Answer 29

Platelet clumps Platelet satellitism Excess EDTA more than 3 hours at room temperature