activity 2 LABORATORY Flashcards
to confirm if patient is suffering from thrombocytopenia
direct platelet count
MPV
2-20 fL
this is where the morphology of the cell can be observed.
smear
Light microscopy- Tocantins method uses?
uses rees and ecker fluid
Phase-contrast microscopy: Brecker-Cronkite Method uses what reagent?
uses 1% Ammonium oxalate
uses Hema analyzer or Hematology analyzer
automated
EDTA BLOOD
- Stable for 5 hours at 20 degree celcius
- Stable for 24 hours at 4 degree celcius
device used for counting cells
HEMACYTOMETER
Count platelets in 4 lARge W square
LIGHT MICROSCOPY
Count platelets in 10 RBC/small
PHASE CONTRAST MICROSCOPY
read in 4 quadrants
TOCANTINS
serves as indicator if the chamber is successfully filled
COVER SLIP
moist chamber (incubate) for how many minutes
15 minutes
to allow platelet to settle
incubation
what happens if the chamber is not incubated
- drying
- not settle
platelets have a 2-4 um diameter.
Phase Contrast Microscopy or Brecker-Cronkite method
allow the platelets to be distinguished from debris which is often refractile.
purple sheen
plt are smaller 1/10th the size of the RBC , round , oval , and elongated particles with light blue stain.
Tocantins method or Rees and Ecker Fluid-
REESE ECKER FLUID composition
- Sodium Citrate
- Formalin
- Brilliant Cresyl Blue
what is the anticoagulant in the composition of reese and ecker fluid
Sodium citrate
what is the preservatine in the composition of reese and ecker fluid
formalin
what is the staining reagent in the composition of reese and ecker fluid
Brilliant Cresyl Blue
BRECKER-CRONKITE composition
1% Ammonium Oxalate
ensures background clearing by hemolizing the red blood cells
1% Ammonium Oxalate
counting chamber of brecker cronkite method
spencer-Briteline
IDEAL DILUTING FLUID
- Should prevent platelet from adhering to the glass surface
- because it might dry and might not be counted - Should prevent platelet from aggregating
- Should not promote premature platelet hemolysis
- Should make platelets readily visible under microscope
K3 EDTA (.22%) > NH4 OXALATE (.44%) + Crystal violet (.75%)
UNOPETTE METHOD
FONIO’S METHOD - uses 14% Magnesium sulfate as diluent
INDIRECT METHOD
SOURCES OF ERRORS:
Platelet clumps
Platelet satellitism
Excess EDTA
more than 3 hours at room temperature