PRELAB 6-7 Flashcards
Inappropriate container
a) Glass surface
b) Polystyrene tubes/ glass tube with silicone- preferred
Improper temperature: labile factors @room temp: _______ @ref temp: _________
@room temp - V,VIII
@ref temp - VII, XI
Principle: when venous blood is put into a glass tube, it will form a solid clot.
Lee and white method
Principle: Presence of an activator (DIATOME) and by keeping the blood constant at 37 deg celsius, the test is more reliable and rapid.
Activated Clotting Time
Time required for blood to clot after the addition of calcium
Plasma Recalcification Time (PRT)
Monitors heparin therapy
Activated partial thromboplastin time
-monitors oral anticoagulant with COUMADIN
PROTHROMBIN TIME
source of reptilase enzyme (thrombin like substance)
Bothrops atrox
Dysfibrinogenemia : reptilase time
Greatly Prolonged
Measures all coagulation factors needed for the generation of Intrinsic prothrombinase except for Calcium and PPL
Activated partial thromboplastin time
Principle: When tissue extract of thromboplastin (complete) is added to PPP, along with Calcium, it reacts with FVII to activate FX
PROTHROMBIN TIME (Quick’s Test)
-standardized way of reporting PT in monitoring anticoagulant therapy
International Normalized Ratio
is a thromboplastin-like substance
“Vipera ruselli”
Principle: Addition of the venom bypasses the activation of VII and directly activates FX.
STYPVEN/RUSELL’s VIPER VENOM
Otherwise known as “Fibrinogen Deficiency Test”
THROMBIN TIME
DUKERT TEST (5M Urea Solubility Test)
Test for FXIII deficiency
Principle: clot formed in normal plasma is insoluble in 5M urea during a 24 hr incubation.
DUKERT TEST
-screening procedure for the assessment of fibrinolysis
EUGLOBULIN TEST
-distinguishes primary vs secondary fibrinolysis
-screening procedure in diagnosing DIC
PROTAMINE SULFATE TEST and ETHANOL GELATION TEST
-measures fibrinolysis but not fibrinogenolysis
-uses latex bead with monoclonal ab to D dimer
D-Dimer Test
Test for FSP
THROMBO-WELCOTEST
