LAB EVALUATION CONTINUATION Flashcards
Venom ——- is a thromboplastin-like substance
“Vipera ruselli”
Addition of the venom bypasses the activation of — and directly activates FX.
VII
STYPVEN/RUSELL’s VIPER VENOM NORMAL VALUES
NV: 6-10 seconds
What is the function of Dilute Russel Viper Venome time (dRVVT)?
DETECTS LUPUS ANTICOAGULANT
Otherwise known as “Fibrinogen Deficiency Test”
THROMBIN TIME
THROMBIN TIME PRINCIPLE
Principle: Addition of thrombin bypasses all coagulation pathways except:
polymerization of Fibrinogen
-measures the conversion of —— to fibrin
fibrinogen
THROMBIN TIME NORMAL VALUE
NV: 17-25 seconds
Fibrinogen level IN tHROMBIN TIME
75-100 mg/dL
Presence of Heparin and FDP
THROMBIN TIME
IN THROMBIN TIME Normally prolonged in
newborn and multiple myeloma
source of reptilase enzyme (thrombin like substance)
Bothrops atrox
Principle: Reptilase bypasses all coagulation pathways except the
polymerization of fibrinogen and the monomers.
mode of action IN REPTILASE TIME
joins the monomers end to end
NORMAL VALUE IN REPTILASE TIME
NV: 18-20 seconds
WHAT IS PROLONGED IN REPTILASE TIME:
Fibrinogen deficiency Presence of FSP
Test for FXIII deficiency
DUKERT TEST (5M Urea Solubility Test)
Principle: clot formed in normal plasma is —– during a —–
insoluble in 5M urea
24 hr incubation
Indicator: If —– is deficient, the clot is dissolved in less than —- by urea
Factor XIII
24 hours
-screening procedure for the assessment of fibrinolysis
-Euglobulin fraction
EUGLOBULIN TEST
Euglobulin fraction:
Plasminogen, activators of plasminogen, fibrinogen
Plasma euglobulins are precipitated with
1 %Hac.
The clot is incubated and the time for complete lysis @ ——- is measured.
37 deg celcius
normal valu for euglobulin test
NV: 2-4 hours
time for abnormal fibrinolytic activity
<2 hours
Primary Fibrinolysis
-Rapid clot breakdown
-increase in the circulating tPA binding to fibrin
-Excess tPA
-decreased hepatic clearance
-decreased fibrinolytic inhibitors
Secondary Fibrinolysis
-Secondary to systemic hypercoagulability
-systemic or microvascular
-not localized
-commonly assoc with DIC
-detects fibrin monomers
-distinguishes primary vs secondary fibrinolysis
PROTAMINE SULFATE TEST and ETHANOL GELATION TEST
Principle: —— displaces the secondary FDPs from fibrin monomer and spontaneously form gel-like clots (paracoagulation)
Protamine sulfate/ 50% ethanol
Normal in protamin sulfate test
no gel formation
D-d arises from the
degradation of cross linked fibrin;
-uses —– with monoclonal ab to D dimer
latex bead
what increases in protamine sulfate
DIC
Thrombosis
Phlebitis
Test for FSP
THROMBO-WELCOTEST
-Whole blood is added to thrombin to ensure
complete ——– after incubation,
clotting and soya bean enzyme inhibitor
