LAB EVALUATION CONTINUATION

Question 1

Venom ——- is a thromboplastin-like substance

Answer 1

“Vipera ruselli”

Question 2

Addition of the venom bypasses the activation of — and directly activates FX.

Answer 2

VII

Question 3

STYPVEN/RUSELL’s VIPER VENOM NORMAL VALUES

Answer 3

NV: 6-10 seconds

Question 4

What is the function of Dilute Russel Viper Venome time (dRVVT)?

Answer 4

DETECTS LUPUS ANTICOAGULANT

Question 5

Otherwise known as “Fibrinogen Deficiency Test”

Answer 5

THROMBIN TIME

Question 6

THROMBIN TIME PRINCIPLE
Principle: Addition of thrombin bypasses all coagulation pathways except:

Answer 6

polymerization of Fibrinogen

Question 7

-measures the conversion of —— to fibrin

Answer 7

fibrinogen

Question 8

THROMBIN TIME NORMAL VALUE

Answer 8

NV: 17-25 seconds

Question 9

Fibrinogen level IN tHROMBIN TIME

Answer 9

75-100 mg/dL

Question 10

Presence of Heparin and FDP

Answer 10

THROMBIN TIME

Question 11

IN THROMBIN TIME Normally prolonged in

Answer 11

newborn and multiple myeloma

Question 12

source of reptilase enzyme (thrombin like substance)

Answer 12

Bothrops atrox

Question 13

Principle: Reptilase bypasses all coagulation pathways except the

Answer 13

polymerization of fibrinogen and the monomers.

Question 14

mode of action IN REPTILASE TIME

Answer 14

joins the monomers end to end

Question 15

NORMAL VALUE IN REPTILASE TIME

Answer 15

NV: 18-20 seconds

Question 16

WHAT IS PROLONGED IN REPTILASE TIME:

Answer 16

Fibrinogen deficiency Presence of FSP

Question 17

Test for FXIII deficiency

Answer 17

DUKERT TEST (5M Urea Solubility Test)

Question 18

Principle: clot formed in normal plasma is —– during a —–

Answer 18

insoluble in 5M urea
24 hr incubation

Question 19

Indicator: If —– is deficient, the clot is dissolved in less than —- by urea

Answer 19

Factor XIII
24 hours

Question 20

-screening procedure for the assessment of fibrinolysis
-Euglobulin fraction

Answer 20

EUGLOBULIN TEST

Question 21

Euglobulin fraction:

Answer 21

Plasminogen, activators of plasminogen, fibrinogen

Question 22

Plasma euglobulins are precipitated with

Answer 22

1 %Hac.

Question 23

The clot is incubated and the time for complete lysis @ ——- is measured.

Answer 23

37 deg celcius

Question 24

normal valu for euglobulin test

Answer 24

NV: 2-4 hours

Question 25

time for abnormal fibrinolytic activity

Answer 25

<2 hours

Question 26

Primary Fibrinolysis
-Rapid clot breakdown
-increase in the circulating tPA binding to fibrin
-Excess tPA
-decreased hepatic clearance
-decreased fibrinolytic inhibitors

Answer 26

Secondary Fibrinolysis
-Secondary to systemic hypercoagulability
-systemic or microvascular
-not localized
-commonly assoc with DIC

Question 27

-detects fibrin monomers
-distinguishes primary vs secondary fibrinolysis

Answer 27

PROTAMINE SULFATE TEST and ETHANOL GELATION TEST

Question 28

Principle: —— displaces the secondary FDPs from fibrin monomer and spontaneously form gel-like clots (paracoagulation)

Answer 28

Protamine sulfate/ 50% ethanol

Question 29

Normal in protamin sulfate test

Answer 29

no gel formation

Question 30

D-d arises from the

Answer 30

degradation of cross linked fibrin;

Question 31

-uses —– with monoclonal ab to D dimer

Answer 31

latex bead

Question 32

what increases in protamine sulfate

Answer 32

DIC
Thrombosis
Phlebitis

Question 33

Test for FSP

Answer 33

THROMBO-WELCOTEST

Question 34

-Whole blood is added to thrombin to ensure
complete ——– after incubation,

Answer 34

clotting and soya bean enzyme inhibitor