Activity 6 LABORATORY Flashcards
1
Q
Laboratory Activity 6
A
WHOLE BLOOD COAGULATION TIME
2
Q
This measures the time required for blood to clot after it has been removed from the body. This is a measure of the overall intrinsic and common pathways of coagulation.
A
WHOLE BLOOD COAGULATION TIME
3
Q
Specimen required
A
Micro Method – capillary blood
Macro Method – Whole blood
4
Q
Micro Method
A
slide method
5
Q
Macro Method
A
Lee and White Method
6
Q
- Do a ——-. Start stopwatch at the time of ——–
A
finger puncture
appearance of blood from the puncture
7
Q
- Place on a clean glass slide —— of blood.
A
3 separate drops
8
Q
- Allow to stand for —– at ——. Check clot formation by drawing the blood with a ——– and observe for thread formation.
A
2 minutes
room temperature
needle or lancet
9
Q
- Record —— from the start to fibrin thread formation.
A
clotting time
10
Q
- Label ——- tubes 1, 2 and 3.
A
three uniformly sized
11
Q
- Make a clean venipuncture using —– and note the time at which blood enters the syringe. Draw —- of blood.
A
20-gauge needle
four (4) ml
12
Q
- Carefully dispense —– to tube 3, then 1 ml to tube 2 and 1ml to tube 1. Discard the remaining blood.
A
1 ml
13
Q
- Incubate all tubes in a water bath at —–
A
37 0C (+ 0.5 0C )
14
Q
- At exactly ——, tilt tube number 1 to a —— and observe for clotting. Repeat ——- until tube can be completely inverted without spilling contents. Note time of clotting.
A
5 minutes
45 degree angle
every 30 seconds
15
Q
- After—- tube 1 is clotted, proceed to tube 2 and repeat the preceding procedures. Repeat procedures with tube 3.
A
30 seconds
