Activity 3 LAB Flashcards
used to see the platelet morphology, RBC morphology, and WBC morphology in blood smear.
It is also one of the confirmatory tests for low platelets. we perform here blood film and blood smear
PLATELET ESTIMATION
2 TYPES OF SLIDES THAT WE USED IN PERIPHERAL BLOOD FILM/BLOOD SMEAR:
- Spreader or push slide
- Film slide (stationary)
the blood that is being dropped in the slide is
2-3mm by using an automated pipette
Size of slide
Slide is 75mm long and 25mm width
Length of film
The film is two thirds to three fourths the length of the slide
Film shape
The film is finger shaped, very slightly rounded at the feather edge (widest area for examination), not bullet shaped; this provides the widest area for examination.
Visible on slide
The lateral edges of the film are visible.
When the slide is held up to the light, the thin portion (feather edge) of the film has a
“rainbow” appearance
Angle of the spreader to stationary
30-45 degrees
Where fibrin strands formation is seen.
10X OBJECTIVE EXAMINATION
You can perform WBC estimate and the perfect WBC morphology is also seen here.
40X HIGH-DRY OBJECTIVE LENS
You can check for RBC morphology, WBC morphology, and platelet count
100X OIL IMMERSION OBJECTIVE EXAMINATION
How many fields are counted
10 oil immersion field is counted
8-20/OIF:
expect adequate platelet count
< 5/IOF:
expect lower platelet count
20:
expect higher platelet count
0 – 49,000
Marked decrease
50,000 – 99,000
Moderate decreased
100,000 – 149,000
Slight decreased
150,000 – 199,000
Low normal
200,000 – 400,000
Normal
401,000 – 599,000
Slight increase
600,000 – 800,000
Moderate increase
Above 800,000
Marked increase
Platelet surrounds and adhere around neutrophils.
Platelet satellitism
Specimen needed
Wright- or Giemsa-stained blood smear
Select an area of the blood film in which most
RBCs are separated from one another with minimal overlapping of RBCs and where platelets are not clumped.
Using the —–, count the number of platelets in ,—– and calculate the average number of platelets per field.
100x oil immersion objective
10 consecutive fields
To obtain the platelet estimate per µL or mm3 of blood, multiply the average number of platelets per field by
20,000
Accurate estimates are possible only when there are no platelet clumps, or at most, rare clumps of
2 to 3 platelets
A better estimate is possible using venous blood with —– as an anticoagulant, in which platelets are evenly distributed and where clumping normally does not occur.
EDTA
On average, there are —- platelets per field with — red cells.
8 – 20
200
