Activity 3 LAB

Question 1

 used to see the platelet morphology, RBC morphology, and WBC morphology in blood smear.
 It is also one of the confirmatory tests for low platelets.  we perform here blood film and blood smear

Answer 1

PLATELET ESTIMATION

Question 2

2 TYPES OF SLIDES THAT WE USED IN PERIPHERAL BLOOD FILM/BLOOD SMEAR:

Answer 2

1. Spreader or push slide
2. Film slide (stationary)

Question 3

 the blood that is being dropped in the slide is

Answer 3

2-3mm by using an automated pipette

Question 4

Size of slide

Answer 4

 Slide is 75mm long and 25mm width

Question 5

Length of film

Answer 5

The film is two thirds to three fourths the length of the slide

Question 6

Film shape

Answer 6

The film is finger shaped, very slightly rounded at the feather edge (widest area for examination), not bullet shaped; this provides the widest area for examination.

Question 7

Visible on slide

Answer 7

The lateral edges of the film are visible.

Question 8

When the slide is held up to the light, the thin portion (feather edge) of the film has a

Answer 8

“rainbow” appearance

Question 9

Angle of the spreader to stationary

Answer 9

30-45 degrees

Question 10

Where fibrin strands formation is seen.

Answer 10

10X OBJECTIVE EXAMINATION

Question 11

You can perform WBC estimate and the perfect WBC morphology is also seen here.

Answer 11

40X HIGH-DRY OBJECTIVE LENS

Question 12

You can check for RBC morphology, WBC morphology, and platelet count

Answer 12

100X OIL IMMERSION OBJECTIVE EXAMINATION

Question 13

How many fields are counted

Answer 13

10 oil immersion field is counted

Question 14

8-20/OIF:

Answer 14

expect adequate platelet count

Question 15

< 5/IOF:

Answer 15

expect lower platelet count

Question 16

20:

Answer 16

expect higher platelet count

Question 17

0 – 49,000

Answer 17

Marked decrease

Question 18

50,000 – 99,000

Answer 18

Moderate decreased

Question 19

100,000 – 149,000

Answer 19

Slight decreased

Question 20

150,000 – 199,000

Answer 20

Low normal

Question 21

200,000 – 400,000

Answer 21

Normal

Question 22

401,000 – 599,000

Answer 22

Slight increase

Question 23

600,000 – 800,000

Answer 23

Moderate increase

Question 24

Above 800,000

Answer 24

Marked increase

Question 25

Platelet surrounds and adhere around neutrophils.

Answer 25

Platelet satellitism

Question 26

Specimen needed

Answer 26

Wright- or Giemsa-stained blood smear

Question 27

Select an area of the blood film in which most

Answer 27

RBCs are separated from one another with minimal overlapping of RBCs and where platelets are not clumped.

Question 28

Using the -----, count the number of platelets in ,----- and calculate the average number of platelets per field.

Answer 28

100x oil immersion objective 10 consecutive fields

Question 29

To obtain the platelet estimate per µL or mm3 of blood, multiply the average number of platelets per field by

Answer 29

20,000

Question 30

Accurate estimates are possible only when there are no platelet clumps, or at most, rare clumps of

Answer 30

2 to 3 platelets

Question 31

A better estimate is possible using venous blood with ----- as an anticoagulant, in which platelets are evenly distributed and where clumping normally does not occur.

Answer 31

EDTA

Question 32

On average, there are ---- platelets per field with --- red cells.

Answer 32

8 – 20 200