Retics and Erythrocytes Flashcards
rarely done because of its inaccuracy and questionable necessity.
Manual RBC count
people living at a HIGHER ALTITUDE
RBC count, hemoglobin and hematocrit values are ELEVATED
Glucose penetrates the red blood cell with no energy expenditure
via
CLUT-1
ATP uses for RBC
1) maintenance of shape & deformability
2) energy for active transport of cations
3) monitoring/modulating amount of 2,3-BPG generated
product of EMP
2 ATP molecules
Most common enzyme deficiency of the EMP and most common form of HNSHA
Pyruvate kinase (PK) deficiency
screenig for PK def.
Autohemolysis test
confirmatory for PK def
Quantitative PK assay
Recommended screening test for PK deficiency
PK fluorescent spot test
prevent oxidation of globin
HMS
Aerobic glycolysis
HMS
HMS aka
Pentose Phosphate pathway
Most common enzyme deficiency in the pentose phosphate pathway
Glucose-6-phosphate dehydrogenase (G6PD) deficiency
most
common RBC enzyme defect
Glucose-6-phosphate dehydrogenase (G6PD) deficiency
Possible PBS findings in G6PD def include
heinz bodies
bite cells
favism is seen in what type of G6PD def.
Type II
G6PD-Mediterranean variant
Type II G6PD def
drugs, chemicals and food that may cause hemolysis in G6PD deficiency patients (MUST BE AVOIDED):
- Fava beans
- Red wine
Legumes (ex.: garbanzos, kadyos, munggo) - Blueberry
- Soya food (ex.: taho, tokwa, soy sauce)
- Ampalaya
-Naphthalene (mothballs)
cytochrome b5 reductase
Methemoglobin Reductase Pathway
Methemoglobin Reductase Pathway function
maintains the iron in the heme in its reduced state
Rapoport-Luebering Shun function
for production of 2,3-BPG
2,3-BPG function
binds to hgb and decreases the oxygen affinity of Hgb
affinity of hemoglobin for oxygen is dependent on 5 factors:
pH
o Partial pressure of carbon dioxide
o Concentration of 2,3-bisphosphoglycerate (2,3-BPG)
o Temperature
o Presence of other hemoglobin species that are nonfunctional
Shift to the left
increased affinity to O2 and pH
