PBS Flashcards
Specimen used
EDTA blood
blood smears should be made within ___
2 hours
advantages of EDTA blood smear
- Multiple blood smears may be made.
- The blood smear may be prepared at a later time.
- EDTA prevents platelet clumping
disadvantages of EDTA blood smear
1) Platelet satellitosis
2) EDTA-induced platelet clumping
Platelet satellitosis results in
Pseudothrombocytopenia
Platelet satellitosis is corrected by
using 3.2% sodium citrate
platelet count x 1.1
EDTA-induced platelet clumping results in
1) Pseudothrombocytopenia
2) Pseudoleukocytosis
EDTA-induced platelet clumping is corrected by
using 3.2% sodium citrate
(platelet count x 1.1
WBC count x 1.1)
Best for evaluation of blood cell morphology
Anticoagulant-free blood
MOST FREQUENTLY USED blood film preparation
Manual Wedge Technique)
angle between two slides in Manual Wedge Technique)
30 - 45’ angle
if angle of spreader is too high > 45’
thicker smear
if angle of the spreader is too low < 30 ‘
thinner smear
size of drop of blood in blood film prep
2 - 3 mm
distance of drop of blood from label of slide
1 cm
if too low hct (Anemia)
angle should be raised
if too high hct (PV)
angle is lowered as low as 25’
scanning method from tail to head
Longitudinal
scanning method back & forth serpentine
Battlement
Characteristics of an IDEAL BLOOD SMEAR
Gradual transition from thick to thin area
2/3 to 3/4 the length of the film slide
Finger-shaped
Visible lateral edges
Without irregularities, holes, or streaks
Feather edge has rainbow appearance
Whole drop of blood is picked up and
spread
blood film prep technique that is excellent in WBC distribution
Coverslip technique
sometimes used for BM aspirate smeare prep
Coverslip technique
Automated Methods (blood film prep)
A.) Miniprep
B.) Centrifugal (Spinner) Type
C.) Coulter LH
D.) Sysmex SP-10
B.) Centrifugal (Spinner) Type uses ____ volume of blood
0.2mL
