PBS

Question 1

Specimen used

Answer 1

EDTA blood

Question 2

blood smears should be made within ___

Answer 2

2 hours

Question 3

advantages of EDTA blood smear

Answer 3

1. Multiple blood smears may be made.
2. The blood smear may be prepared at a later time.
3. EDTA prevents platelet clumping

Question 4

disadvantages of EDTA blood smear

Answer 4

1) Platelet satellitosis

2) EDTA-induced platelet clumping

Question 5

Platelet satellitosis results in

Answer 5

Pseudothrombocytopenia

Question 6

Platelet satellitosis is corrected by

Answer 6

using 3.2% sodium citrate

platelet count x 1.1

Question 7

EDTA-induced platelet clumping results in

Answer 7

1) Pseudothrombocytopenia

2) Pseudoleukocytosis

Question 8

EDTA-induced platelet clumping is corrected by

Answer 8

using 3.2% sodium citrate

(platelet count x 1.1

WBC count x 1.1)

Question 9

Best for evaluation of blood cell morphology

Answer 9

Anticoagulant-free blood

Question 10

MOST FREQUENTLY USED blood film preparation

Answer 10

Manual Wedge Technique)

Question 11

angle between two slides in Manual Wedge Technique)

Answer 11

30 - 45’ angle

Question 12

if angle of spreader is too high > 45’

Answer 12

thicker smear

Question 13

if angle of the spreader is too low < 30 ‘

Answer 13

thinner smear

Question 14

size of drop of blood in blood film prep

Answer 14

2 - 3 mm

Question 15

distance of drop of blood from label of slide

Answer 15

1 cm

Question 16

if too low hct (Anemia)

Answer 16

angle should be raised

Question 17

if too high hct (PV)

Answer 17

angle is lowered as low as 25’

Question 18

scanning method from tail to head

Answer 18

Longitudinal

Question 19

scanning method back & forth serpentine

Answer 19

Battlement

Question 20

Characteristics of an IDEAL BLOOD SMEAR

Answer 20

Gradual transition from thick to thin area
 2/3 to 3/4 the length of the film slide
 Finger-shaped
 Visible lateral edges
 Without irregularities, holes, or streaks
 Feather edge has rainbow appearance
 Whole drop of blood is picked up and
spread

Question 21

blood film prep technique that is excellent in WBC distribution

Answer 21

Coverslip technique

Question 22

sometimes used for BM aspirate smeare prep

Answer 22

Coverslip technique

Question 23

Automated Methods (blood film prep)

Answer 23

A.) Miniprep
B.) Centrifugal (Spinner) Type
C.) Coulter LH
D.) Sysmex SP-10

Question 24

B.) Centrifugal (Spinner) Type uses ____ volume of blood

Answer 24

0.2mL

Question 25

Both slide makers & slide stainers

Answer 25

Coulter LH

& Sysmex SP-10

Question 26

nuclear remnants of lymphocytes and a lot found in CLL

Answer 26

Smudge cells

Question 27

Purpose of blood smear staining

Answer 27

for evaluation of cellular morphology

Question 28

fixative

Answer 28

methanol

Question 29

stain

Answer 29

wright or wright giemsa

Question 30

buffer

Answer 30

0.05 M Sodium Phosphate (pH 6.4)

Question 31

Romanowsky stain contains

Answer 31

methylene blue + eosin B or eosin Y

Question 32

most commonly used type of stain in the hematology laboratory

Answer 32

Romanowsky stain

Question 33

examples of Romanowsky-based stain

Answer 33

Wright stain, Giemsa stain, and May-Grunwald stain

Question 34

automated staingin

Answer 34

Midas III,
HEMA-TEK
Coulter LH
Sysmex SP-10

Question 35

Macroscopic appearance of well-stained smear

Answer 35

pink-to-purple

Question 36

microscopic appearance of well stained smear

Answer 36

RBCs: orange to salmon pink

WBC nuclei: purple to blue

Neutrophil cytoplasm: pink to tan

Eosinophil granules: bright-orange

Question 37

RBCs: gray (or blue)
WBCs: too dark
Eosinophil granules: gray

Answer 37

• buffer is too basic
• inadequate rinsing
• heparinized blood

Question 38

RBCs: too pale or are red
WBCs: barely visible

Answer 38

• stain/buffer is too acidic
• under buffering
• over rinsing

Question 39

(presence of more than four times the

number of WBCs per field at the lateral edges

Answer 39

snowplow

Question 40

snowplow is detected in what obective

Answer 40

10x obective