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hema - review > HCT, ESR, OFT > Flashcards

HCT, ESR, OFT Flashcards

1
Q

Hematocrit

A

pertains to the instrument used to determine packed cell volume [PCV]

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2
Q

volume of the red blood cells that have settled

A

packed cell volume/hematocrit

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3
Q

hematocrit

A

object being seen in a diff. position by changing the position of the head of the observer, or as seen by one
eye versus the other eye

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4
Q

-Micro-Hematocrit Tube

A

o Approx. 75 mm long
o Inner bore: 1 .2 mm
o Can hold 0.05 mL of bld.
o Plug: 4 to 6 mm long

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5
Q

-Micro-Hematocrit Tube 2 TYPES:

A

1) Red band

2) blue band

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6
Q

no anticoagulant

A

blue band

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7
Q

with anticoagulant

A

red band

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8
Q

fill microhematocrit tube up to

A

2/3

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9
Q

top layer

A

fatty layer

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10
Q

4th layer

A

packed rbc

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11
Q

2nd layer

A

plasma layer

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12
Q

3rd layer

A

buffy coat

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13
Q

5th & 6th layer

A

wax and clay

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14
Q

microhematocrit spins for ____ at _____

A

5 minutes at 10,000 RPM

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15
Q

2 patient samples run must agree within

A

+/- 0.02 L/L

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16
Q

Trapped plasma may cause the Hct to

A

falsely increased (0.02 L/L)

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17
Q

a calculated value from RBC and MC

A

Automated hematocrit

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18
Q

More trapped plasma in the following:

A 
-Sickle cell anemia
 Hypochromic anemia
 Spherocytosis
 Macrocytosis
 Thalassemia
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19
Q

Some of the causes of

Falsel

A

1) DEHYDRATION
2) Hemoconcentration
3) insufficient centrifugation
4) buffy coat inclusion in the reading

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20
Q

Falsely DECREASED Hematocrit

A

1) hemolysis
2) improper sealing of capillary tube
3) inc. anticoagulation conc.
3) introduction of interstitial fluid

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21
Q

used for checking validity of test results

A

RULE OF THREE

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22
Q

rule of three can only be done on

A

normocytic, normochromic specimens

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23
Q

RULE OF THREE formula

A

3 x RBC = Hgb

3 x Hgb = Hct (+/- 3%)

24
Q

ERYTHROCYTE INDICES

A

1) MCV
2) MCH
3) MCHC

25
Q

Average volume of an individual RBC

26
Q

MCV FORMULA

A

MCV = (Hct / RBC count) x 10

27
Q

MCV Normal value

A

80 to 100 femtoliters

28
Q

Average weight or amount of hemoglobin in an individual RBC

29
Q

MCH follows the

30
Q

MCH FORMULA

A

MCH = (Hgb x RBC ct.) x 10

31
Q

MCH NV

A

26 to 32 picograms

32
Q

Mean concentration of hemoglobin in the average RBC

A

Mean Cell Hemoglobin Concentration (MCHC)

33
Q

MCHC Formula

A

MCHC = (Hgb / Hct) x 100

34
Q

MCHC NV

A

31 to 37 g/dL or %

35
Q

MCHC true increase is only seen

A

Hereditary spherocytosis

36
Q

Value true increase of MCHC

A

35 - 38 g/dL

37
Q

CLASSICALLY defined as increased hematocrit level above norma

A

POLYCYTHEMIA

38
Q

Absolute polycythemia

A

elevated total red cell mass

39
Q

Relative polycythemia:

A

normal total red cell mass, but hematocrit is increased because plasma volume
is decreased
P O L Y C Y T H E M I

40
Q

(mutated VHL gene)

A

Chuvash polycythemia

41
Q

*POLYCYTHEMIA VERA aka

A

Polycythemia Rubra

Vera

42
Q

Polycythemia Vera

A

panmyelosis

43
Q

hallmark sign of PV

44
Q

mutation in _____ - PV

A

JAK2 V617F

45
Q

ERYTHROCYTE SEDIMENTATION RATE

A

Rate of fall of RBCs settling to the bottom of the tube

46
Q

ESR is for detecting and monitoring

A

inflammatory conditions

47
Q

ESR > 37 mm/h

A

prostate cancer

48
Q

ESR of > 28 mm/h

49
Q

ESR >22 mm/h

A

coronary artery disease

50
Q

Methods of ESR Determination

A

1) Westergren
2) Modified Westergren
3) Wintrobe
4) VES-MATIC 20 Instrument

51
Q

stages of RBC sedimentation

A

1) Lag phase
2) decantation phase
3) final settling phase

52
Q

last 10 mins (esr)

A

final settling phase

53
Q

next 40 mins

A

decantation phase

54
Q

first 10 mins

55
Q

“A tilt of as little as 3’

A

cause error of up to

30%.”

56
Q

anticoag in OFT

hema - review (17 decks)

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