HCT, ESR, OFT

Question 1

Hematocrit

Answer 1

pertains to the instrument used to determine packed cell volume [PCV]

Question 2

volume of the red blood cells that have settled

Answer 2

packed cell volume/hematocrit

Question 3

hematocrit

Answer 3

object being seen in a diff. position by changing the position of the head of the observer, or as seen by one
eye versus the other eye

Question 4

-Micro-Hematocrit Tube

Answer 4

o Approx. 75 mm long
o Inner bore: 1 .2 mm
o Can hold 0.05 mL of bld.
o Plug: 4 to 6 mm long

Question 5

-Micro-Hematocrit Tube 2 TYPES:

Answer 5

1) Red band

2) blue band

Question 6

no anticoagulant

Answer 6

blue band

Question 7

with anticoagulant

Answer 7

red band

Question 8

fill microhematocrit tube up to

Answer 8

2/3

Question 9

top layer

Answer 9

fatty layer

Question 10

4th layer

Answer 10

packed rbc

Question 11

2nd layer

Answer 11

plasma layer

Question 12

3rd layer

Answer 12

buffy coat

Question 13

5th & 6th layer

Answer 13

wax and clay

Question 14

microhematocrit spins for ____ at _____

Answer 14

5 minutes at 10,000 RPM

Question 15

2 patient samples run must agree within

Answer 15

+/- 0.02 L/L

Question 16

Trapped plasma may cause the Hct to

Answer 16

falsely increased (0.02 L/L)

Question 17

a calculated value from RBC and MC

Answer 17

Automated hematocrit

Question 18

More trapped plasma in the following:

Answer 18

-Sickle cell anemia
 Hypochromic anemia
 Spherocytosis
 Macrocytosis
 Thalassemia

Question 19

Some of the causes of

Falsel

Answer 19

1) DEHYDRATION
2) Hemoconcentration
3) insufficient centrifugation
4) buffy coat inclusion in the reading

Question 20

Falsely DECREASED Hematocrit

Answer 20

1) hemolysis
2) improper sealing of capillary tube
3) inc. anticoagulation conc.
3) introduction of interstitial fluid

Question 21

used for checking validity of test results

Answer 21

RULE OF THREE

Question 22

rule of three can only be done on

Answer 22

normocytic, normochromic specimens

Question 23

RULE OF THREE formula

Answer 23

3 x RBC = Hgb

3 x Hgb = Hct (+/- 3%)

Question 24

ERYTHROCYTE INDICES

Answer 24

1) MCV
2) MCH
3) MCHC

Question 25

Average volume of an individual RBC

Answer 25

MCV

Question 26

MCV FORMULA

Answer 26

MCV = (Hct / RBC count) x 10

Question 27

MCV Normal value

Answer 27

80 to 100 femtoliters

Question 28

Average weight or amount of hemoglobin in an individual RBC

Answer 28

MCH

Question 29

MCH follows the

Answer 29

MCV

Question 30

MCH FORMULA

Answer 30

MCH = (Hgb x RBC ct.) x 10

Question 31

MCH NV

Answer 31

26 to 32 picograms

Question 32

Mean concentration of hemoglobin in the average RBC

Answer 32

Mean Cell Hemoglobin Concentration (MCHC)

Question 33

MCHC Formula

Answer 33

MCHC = (Hgb / Hct) x 100

Question 34

MCHC NV

Answer 34

31 to 37 g/dL or %

Question 35

MCHC true increase is only seen

Answer 35

Hereditary spherocytosis

Question 36

Value true increase of MCHC

Answer 36

35 - 38 g/dL

Question 37

CLASSICALLY defined as increased hematocrit level above norma

Answer 37

POLYCYTHEMIA

Question 38

Absolute polycythemia

Answer 38

elevated total red cell mass

Question 39

Relative polycythemia:

Answer 39

normal total red cell mass, but hematocrit is increased because plasma volume is decreased P O L Y C Y T H E M I

Question 40

(mutated VHL gene)

Answer 40

Chuvash polycythemia

Question 41

*POLYCYTHEMIA VERA aka

Answer 41

Polycythemia Rubra | Vera

Question 42

Polycythemia Vera

Answer 42

panmyelosis

Question 43

hallmark sign of PV

Answer 43

Plethroa

Question 44

mutation in _____ - PV

Answer 44

JAK2 V617F

Question 45

ERYTHROCYTE SEDIMENTATION RATE

Answer 45

Rate of fall of RBCs settling to the bottom of the tube

Question 46

ESR is for detecting and monitoring

Answer 46

inflammatory conditions

Question 47

ESR > 37 mm/h

Answer 47

prostate cancer

Question 48

ESR of > 28 mm/h

Answer 48

stroke

Question 49

ESR >22 mm/h

Answer 49

coronary artery disease

Question 50

Methods of ESR Determination

Answer 50

1) Westergren 2) Modified Westergren 3) Wintrobe 4) VES-MATIC 20 Instrument

Question 51

stages of RBC sedimentation

Answer 51

1) Lag phase 2) decantation phase 3) final settling phase

Question 52

last 10 mins (esr)

Answer 52

final settling phase

Question 53

next 40 mins

Answer 53

decantation phase

Question 54

first 10 mins

Answer 54

Lag phase

Question 55

“A tilt of as little as 3'

Answer 55

cause error of up to | 30%.”

Question 56

anticoag in OFT

Answer 56

heparin