Reproduction Flashcards
How frequently can semen be harvested for AI?
Every 30-60 minutes
How is semen stress tested?
Progressive motility checked after 20 minutes at room temperature
What volume of fresh semen is required for AI?
0.1ml
Describe how semen is extended for chilling and freezing
Diluted according to sperm count (1:1-1:4) using buffers containing glucose/fructose energy source. If being frozen a cell protectant is added e.g. glycerol.
How does eCG administration affect the ram/buck effect?
Increases testosterone production leading to a more pronounced effect.
When will peak matings occur after using the ram/buck effect.
Two peaks at approximately 18 and 26 days in sheep. (One silent cycle or one and a half silent cycles before mating)
What proportion of females will be synchronised by a single PGF2a injection?
When will they express oestrus?
66%
30-60 hours
How can two PGF2a injections be used to synchronise the whole group?
Second injection after 7-11 days. (Or hand mate and only inject those not mated)
What are the active ingredients of chronogest sponges and CIDR-ovis. How long should they be left in situ?
Flugestone and progesterone
14d and 12d
How soon after sponge/cidr removal should rams be introduced?
36-48h for sponges. Possibly at 24h for cidr-ovis
Whn should eCG be given relative to sponge/CIDR removal?
at time of or 24-48h after.
How can PGF2a be used to tighten the cidr/sponge sync
administer 24h prior to removal to ensure sharp fall in progesterone in case a CL is present.
Can breeding season manipulation be performed in lactating goats?
Yes but not in the first 4 months of lactation
How long before intended lambing should regulin (melatonin) be implanted?
29 weeks
How long after regulin implantation should tups (potentially vasectomised) be introduced?
30-40 days
When using melatonin in implants to advance breeding season, how long after tup introduction will matings start and when will the peak be?
matings from 14 days, peak around 25-30 days
How long after progestin removal should timed AI be performed?
55h
What routes are there for AI, what are the success rates?
Vaginal 15-30%
Cervical 35-50%
Transcervical 40-70%
Laparoscopic 20-90% (highly operator dependent)
How many embryos can be expected per flush?
8-10
What is the success rate of embryo transfer
60-80%
How long does spermatogenesis take? How long after this does transport to epididymis take?
49-60 days. Further 2 weeks
What targets are there for scrotal circumference in rams and bucks?
Ram lambs: 30cm
Ram shearlings: 33cm
Ram: 36cm
Dairy bucks 28cm
Heavy meat bucks: 34-36cm
Wht effect does twinning have on sperm production?
Male-male twins have higher than male-female twins
At what proportion of adult bodyweight does puberty occur?
60%
After how many days does the ovine placenta start to produce its own progesterone?
75 days
What is normal gestation for ewes and does?
ewes: 145-150
does: 147-155
From how many days can transrectal ultrasound detect pregnancy?
24d
When is the best time to assess twinning by ultrasound?
45-90d
What targets are there for sperm motility and morphology?
> 70% progressive motility
90% normal morphology
How can sperm morphological abnormalities sugget the location of pathology?
Head and acrosome defects associated with testes, tail defects assocaited with epididymes
How can hormone assays be used to detect pregnancy?
Estrone sulfate assay after d50
Prgnancy-specific protein B after d30
Low progesterone can confirm non-pregnancy but cannot be used to confirm pregnancy. (Penside tests for cattle work in goats)
How can parturiaion be induced?
PGF2a in goats
16mg dexamethasone in sheep, possibly after pre-priming with increasing dose (2mg, 2mg, 2mg, 4mg, 16mg) to help foetal maturation
How long is the oestrus cycle in goats and sheep?
17d in sheep, 21d in goats
How long does oestrus last in goats and sheep
15-45h in sheep
24-72h in goats
List causes for vaginitis
Orf, CHV-1, mycoplasma, ascending bacterial infections, ectopic mammary tissue
List 3 causes for cystic ovaries in goats
hormonal manipulation for superovulation
Phosphorous deficiency
Oestrogenic feed
Which hormones are altered by granulosa cell tumours?
Elevated oestrogen and testosterone
List a predisposing factor for freemartinism
Lots of multiples. Still very rare though
Describe the pathogenesis of a varicocoele
Dilatation and thrombosis of the internal spermatic vein leads to painful swelling of the spermatic cord
How is Brucella ovis diagnosed?
ELISA. Repeated in 4-8 weeks if negative.
List the main causes of epididymititis
B. ovis
Actinobacillus seminis
Histophilus/haemophilus spp
In which part of the epididymis are sperm granulomas found
The head
List 7 causes of testicular hypoplasia
congenital
Season
Zn decifiency
Hypothyroidism (I deficiency)
Starvation
Systemic dz
Cold/heat stress
What karyotype are caprine pseudohermaphrodites? What gonads do they posess? What genitalia?
XX. Intra-abdominal testicles. Female genitalia that progressively masculinises.
What phenotype is associated with hermaphrodism?
Polling in dairy goats
List 6 causes of non-infectious abortion
Stress, Nutrition, Pharmaceutical reactions, Toxic plants, sheep-goat hybrids, chromosomal rearrangements
Which mineral deficiencies are assocaited with foetal resorption?
Iodine and selenium