RATIONALE FINALS Flashcards
Biochemically, the Enterobacteriaceae are gram-negative rods that:
A. Ferment glucose, reduce nitrate to nitrite, and are oxidase negative
B. Ferment glucose, produce indophenol oxidase, and form gas
C. Ferment lactose and reduce nitrite to nitrogen gas
D. Ferment lactose and produce indophenol oxidase
A
Which genera of the Enterobacteriaceae are known to cause diarrhea and are considered enteric
pathogens?
A. Enterobacter, Klebsiella, Providencia, and Proteus
B. Escherichia, Salmonella, Shigella, and Yersinia
C. Pseudomonas, Moraxella, Acinetobacter, and Aeromonas
D. Enterobacter, Citrobacter, and Morganella
B
MacConkey agar: little normal flora with many non-lactose-fermenting colonies
Hektoen enteric agar: many blue-green colonies
Campylobacter blood agar and C. difficile agar: No growth
Oxidase: colorless Indole: yellow
Urease: orange SIM: no brush-like appearance, no blackening
ONPG: colorless
The most likely identification is:
a. Shigella dysenteriae
b. Salmonella gallinarum
c. Shigella flexneri
d. Escherichia coli
A
A wound aspirate culture from a nursing home patient grew gram-negative rods on MacConkey
agar as pink to dark pink oxidase-negative colonies. Given the following results, which is the most
likely organism?
TSI = A/A
VP = red
Urease = magenta
Indole = yellow
Citrate = blue
MR = yellow
H2S = No blackening
Antibiotic susceptibility: resistant to carbapenems and ampicillin
A. Serratia marcescens
B. Hafnia alvei
C. Enterobacter cloacae
D. Klebsiella pneumoniae
D
A patient with a nosocomial pneumonia has a sputum Gram stain that shows many neutrophils and
numerous small gram-negative coccobacilli. The organism grew in 24 hours as a mucoid, nonhemolytic colony on blood agar and a colorless colony on a MacConkey agar. The organism had
the following characteristics:
Oxidase: negative Nitrate: negative
Catalase: positive ONPG: negative
Nitrate: negative Ornithine decarboxylase: negative
Lysine decarboxylase: negative
A. Burkholderia mallei
B. Stenotrophomonas maltophilia
C. Acinetobacter baumannii
D. Pseudomonas putida
C
A college student attended a beach party where raw oysters and other shellfish were consumed.
The next day, he had symptoms of septicemia. The blood cultures grew gram-negative bacilli with
the following characteristics:
Oxidase: positive
MacConkey agar: pink colonies
TCBS: green colonies
O/129 (150 ug): susceptible
String test: with string formation
The most likely organism is:
A. Vibrio alginolyticus
B. Plesiomonas shigelloides
C. Vibrio vulnificus
D. Aeromonas hydrophilia
C
Cultures obtain from a dog bite wound produce yellow, tan, and slightly pink colonies on blood and
chocolate agar with margin of fingerlike projections appearing as a film around colonies. Given the
following results at 24 hours, which is the most likely organism?
Oxidase=colorless catalase=no bubbles
Motility: haze on solid surface Nitrate reduction: yellow
Esculin hydrolysis: blackening Indole: yellow
A. Actinobacter species
B. Capnocytophaga species
C. Eikenella species
D. Pseudomonas specie
B
A slow growing, orange pigmented, acid fast bacillus was isolated from a cervical lymph node of a
child with symptoms of cervical adenitis. With the following biochemical test results:
Catalase: positive ->45mm Nitrate reduction: pink
Urease: pink Tween 80: yellow
The most likely etiological agent in this case would be
A. Mycobacterium avium-intracellulare
B. Mycobacterium chelonei
C. Mycobacterium fortuitum
D. Mycobacterium scrofulaceum
D
A 75-year-old woman presented with a history of decreased appetite, weight loss, and progressive
decline in mental status over a period of 1 week. Laboratory data included the following: increased
WBC count, decreased platelet count, urinalysis showed no evidence of infection. 4 blood cultures
obtained revealed a green pigmented colony, oxidase positive, non-fermenting gram-negative
bacilli with the following biochemical test results:
O/F: open tube: yellow, closed tube: green Nitrate reduction: yellow
Catalase: with bubbles formation Gelatin hydrolysis: gel solidifies
ONPG: yellow Esculin hydrolysis: no blackening
The most likely microorganism is:
A. Pseudomonas fluorescens
B. Pseudomonas stutzeri
C. Pseudomonas luteola
D. Pseudomonas putida
D
Which of the following microorganisms is/are belong to Non-TB Mycobacterium?
I. Mycobacterium caprae
II. Mycobacterium marinum
III. Mycobacterium tuberculosis
IV. Mycobacterium gordonae
A. I, III
B. II, IV
C. I, II, IV
D. III
B
Which of the following statements is/are correct about the classification of atypical mycobacteria
I. Scotochromogen develops yellow to orange pigment in dark or light exposure
II. Group IV are rapid growers, grow on medium after more than 7 days of incubation.
III. Photochromogen develops yellow pigment in the dark
IV. Non-chromogen develops tan colonies
A. II, IV
B. I, IV
C. II, III
D. I, III
B
Smooth gray colonies showing no hemolysis are recovered from an infected cat scratch on blood
and chocolate agar but fail to grow on MacConkey agar. The organisms are gram-negative
pleomorphic rods that are both catalase and oxidase positive and strongly indole positive. The
most likely organism is:
A. Capnocytophaga spp.
B. Pasteurella spp.
C. Proteus spp.
D. Pseudomonas spp.
B
A gram-negative coccobacillus was isolated from an infected cat bite. The isolate grew on blood
agar with a musty smell, but did not grow on MacConkey agar. It was catalase, indole, and oxidase
positive and negative to urease. Which one of the following is the most likely identity of this isolate?
A. Pasteurella canis
B. Pasteurella multocida
C. Eikenella corrodens
D. Pasteurella bettyae
B
A urine culture had the following culture results:
Sheep blood: swarming
Columbia CNA: no growth
MacConkey:
1. >100,000 CFU/mL nonlactose-fermenter
2. >100,000 CFU/mL nonlactose-fermenter with red pigment
The isolates from MacConkey agar had the following biochemical reactions:
Biochemical tests Isolate 1 Isolate 2
TSI Alkaline/acid Alkaline/acid
Urea Positive Negative
TDA Positive Negative
H2S Positive Negative
The organisms are most likely:
a. Proteus vulgaris and Enterobacter cloacae
b. Proteus mirabilis and Serratia marcescens
c. Morganella morganii and Klebsiella pneumoniae
d. Providencia stuartii and Serratia liquefaciens
B
A child was bitten on the arm by her sibling and the resulting wound grew a slender gram-negative
bacillus that has the following characteristics:
Growth on SBA: colonies that “pit” the agar Colonies odor: like bleach
Catalase: negative Oxidase: positive
TSI: no growth
The identification of this organism is:
A. Aggregatibacter aphrophilus
B. Eikenella corrodens
C. Cardiobacterium hominis
D. Kingella oralis
B