1.2.1 Lab Diagnosis for Haemophilus

Question 1

How the specimen for Haemophilus is being collected?

Answer 1

Swab premoistened with sterile phosphate buffered saline, Stuart’s or Amie’s Swab

Question 2

Culture media for Haemophilus?

Answer 2

Chocolate Agar
Chocolate Agar with Bacitracin
Chocolate Agar with 1% IsoVitaleX
Nairobi Biplate Medium

Question 3

Why is chocolate Agar is the media of Choice for Haemophilus?

Answer 3

X and V factor

Question 4

What is the function of bacitracin with Chocolate agar?

Answer 4

Inhibits the growth of oral respiratory normal flora

Question 5

Chocolate agar with 1%IsoVitaleX is used for what bacteria?

Answer 5

H. ducreyi and H. aegypticus

Question 6

Nairobi Biplate medium is specific for what Haemophilus?

Answer 6

H. ducreyi

Question 7

Composition of Nairobi Biplate medium

Answer 7

GC agar base with 2% bovine hemoglobin
5% fetal calf serum
MHA with 5% chocolatized horse blood
Vancomycin

Question 8

Function of Vancomycin in nairobi Biplate medium?

Answer 8

Resistant for H. ducreyi so it can grow while it can inhibit other microorganisms (Especially Gram positive)

Question 9

What is the Incubation for Haemophilus spp. (Except ducreyi)?

Answer 9

5-10% Co2 at 35 -37C

24-72 hrs

Question 10

Incubation for H. ducreyi?

Answer 10

5-10% Co2 at 33 C with high humidity

7 days

Question 11

Small, Gram-negative coccobacilli to long filaments

Encapsulated

Answer 11

Haemophilus spp

Question 12

Gram negative coccobacilli that appear as the following genital lesions?

School of Fish
Railroad tracks
Fingerprints

Answer 12

H. ducreyi

Question 13

Colonial morphology:

Non hemolytic
Translucent, tannish, moist, smooth, and convex
Mousy / Bleach like odor in CHOC agar

Answer 13

H. influenzae

Question 14

In H. influenzae, what is the indication if there is mucoid?

Answer 14

Encapsulated strains

Question 15

Colonial morphology:
Small, Flat, Smooth, Transparent to opaque
Clumpy, non-homogenous appearance in saline

(Difficult to remove in CHOC)

Answer 15

H. ducreyi

Question 16

Haemophilus spp grow on MAC?

Answer 16

False

Question 17

Lab diagnosis for Haemophilus?

Answer 17

Satellitism test
X and V test
Porphyrin test

Question 18

Use for routine identification or differentiation of Haemophilus spp.

Answer 18

Satellitism test

Question 19

How to streak in Satellitism?

Answer 19

Staphylococcus streak

Question 20

Haemophilus is streaked with S. aureus, S. pneumoniae, and Neisseria (All are V factor producer)

Answer 20

Staphylococcus streak

Question 21

What is the positive indicator for Satellitism test?

Answer 21

Moist, Small “Dewdrop” colonies surrounding the S. aureus colonies

Question 22

What bacteria is negative in Satellitism test?

Answer 22

H. haemolyticus
H. ducreri

Question 23

What media is used in Satellitism test?

Answer 23

SBA

Question 24

Incubation for Satellitism test?

Answer 24

72 hrs at 35 -37C

Question 25

What are the Haemophilus that are positive in both X and V factor?

Answer 25

H. influenzae H. aegypticus H. haemolyticus

Question 26

What are the haemophilus that are positive in V factor?

Answer 26

H. parainfluenzae H. paraphrophilus H. parahaemolyticus

Question 27

What are the haemophilus that are positive in X factor?

Answer 27

H. ducreyi

Question 28

What are the haemophilus that are Negative in both X and V factor?

Answer 28

H. aphrophilus

Question 29

What is the Media use for X and V factor?

Answer 29

MHA

Question 30

What is the reagent for Porphyrin tesT?

Answer 30

Aminolevulinic acid

Question 31

NOTE: Aminolevulinic acid -> Porphyrin -> Hemin

Question 32

Phorphobilinogen is detected using?

Answer 32

Kovac's reagent

Question 33

What contains in Kovac's reagent?

Answer 33

P-dimethylaminobenzaldehyde

Question 34

Positive indicator for Porphyrin test?

Answer 34

FLuoresce reddish orange at UC light (360nm)

Question 35

How many hrs of incubation for prophyrin test?

Answer 35

4 hrs

Question 36

Positive in Porphyrin test indicates?

Answer 36

Bacteria can produce X factor (Hemin)

Question 37

What do you call the organisms that is positive in Porphyrin test?

Answer 37

Heme producing species

Question 38

What are the bacteria that are positive in Hemolysis?

Answer 38

H. haemolyticus H. parahaemolyticus

Question 39

Organisms that are positive in ALA?

Answer 39

H. parainfluenza H. parahaemolyticus H. paraphrohaemolyticus H. aphrophilus A. segnis HINT: Lahat sila negative sa X factor since kaya nila mag produce ng sarili nilang X factor