1.2.1 Lab Diagnosis for Haemophilus Flashcards
How the specimen for Haemophilus is being collected?
Swab premoistened with sterile phosphate buffered saline, Stuart’s or Amie’s Swab
Culture media for Haemophilus?
Chocolate Agar
Chocolate Agar with Bacitracin
Chocolate Agar with 1% IsoVitaleX
Nairobi Biplate Medium
Why is chocolate Agar is the media of Choice for Haemophilus?
X and V factor
What is the function of bacitracin with Chocolate agar?
Inhibits the growth of oral respiratory normal flora
Chocolate agar with 1%IsoVitaleX is used for what bacteria?
H. ducreyi and H. aegypticus
Nairobi Biplate medium is specific for what Haemophilus?
H. ducreyi
Composition of Nairobi Biplate medium
GC agar base with 2% bovine hemoglobin
5% fetal calf serum
MHA with 5% chocolatized horse blood
Vancomycin
Function of Vancomycin in nairobi Biplate medium?
Resistant for H. ducreyi so it can grow while it can inhibit other microorganisms (Especially Gram positive)
What is the Incubation for Haemophilus spp. (Except ducreyi)?
5-10% Co2 at 35 -37C
24-72 hrs
Incubation for H. ducreyi?
5-10% Co2 at 33 C with high humidity
7 days
Small, Gram-negative coccobacilli to long filaments
Encapsulated
Haemophilus spp
Gram negative coccobacilli that appear as the following genital lesions?
School of Fish
Railroad tracks
Fingerprints
H. ducreyi
Colonial morphology:
Non hemolytic
Translucent, tannish, moist, smooth, and convex
Mousy / Bleach like odor in CHOC agar
H. influenzae
In H. influenzae, what is the indication if there is mucoid?
Encapsulated strains
Colonial morphology:
Small, Flat, Smooth, Transparent to opaque
Clumpy, non-homogenous appearance in saline
(Difficult to remove in CHOC)
H. ducreyi
Haemophilus spp grow on MAC?
False
Lab diagnosis for Haemophilus?
Satellitism test
X and V test
Porphyrin test
Use for routine identification or differentiation of Haemophilus spp.
Satellitism test
How to streak in Satellitism?
Staphylococcus streak
Haemophilus is streaked with S. aureus, S. pneumoniae, and Neisseria (All are V factor producer)
Staphylococcus streak
What is the positive indicator for Satellitism test?
Moist, Small “Dewdrop” colonies surrounding the S. aureus colonies
What bacteria is negative in Satellitism test?
H. haemolyticus
H. ducreri
What media is used in Satellitism test?
SBA
Incubation for Satellitism test?
72 hrs at 35 -37C
What are the Haemophilus that are positive in both X and V factor?
H. influenzae
H. aegypticus
H. haemolyticus
What are the haemophilus that are positive in V factor?
H. parainfluenzae
H. paraphrophilus
H. parahaemolyticus
What are the haemophilus that are positive in X factor?
H. ducreyi
What are the haemophilus that are Negative in both X and V factor?
H. aphrophilus
What is the Media use for X and V factor?
MHA
What is the reagent for Porphyrin tesT?
Aminolevulinic acid
NOTE: Aminolevulinic acid -> Porphyrin -> Hemin
Phorphobilinogen is detected using?
Kovac’s reagent
What contains in Kovac’s reagent?
P-dimethylaminobenzaldehyde
Positive indicator for Porphyrin test?
FLuoresce reddish orange at UC light (360nm)
How many hrs of incubation for prophyrin test?
4 hrs
Positive in Porphyrin test indicates?
Bacteria can produce X factor (Hemin)
What do you call the organisms that is positive in Porphyrin test?
Heme producing species
What are the bacteria that are positive in Hemolysis?
H. haemolyticus
H. parahaemolyticus
Organisms that are positive in ALA?
H. parainfluenza
H. parahaemolyticus
H. paraphrohaemolyticus
H. aphrophilus
A. segnis
HINT: Lahat sila negative sa X factor since kaya nila mag produce ng sarili nilang X factor
