2.3.2 Specimen collection and handling Flashcards
1
Q
Specimen?
A
Respiratory secretion (Sputum, Bronchial aspirate) with Acid treatment
Blood
Urine
2
Q
How is acid treatment prepared?
A
1:10 dilution
with 0.2 KCl-HCl
Stand for 5 mins
3
Q
This happens when an overgrowth of the normal flora from the respiratory tract will occur
A
Without acid treatment
4
Q
Only legionella grow on the agar
A
With acid treatment
5
Q
How do you release Legionella pneumophilia in Blood?
A
Lysis centrifugation
6
Q
This is done in case of delay processing (Temp)
A
Respiratory samples (-70C)
Urine (2-8C) or Frozen at -20C
7
Q
What is done in urine?
A
Antigen detection