1.1.1 Haemophilus spp

Question 1

Type of organisms that does not require complex nutrients

Answer 1

Nonfastidious (Fast grower)

Fermenters (63%)
Nonfermenters (20.3%)

Question 2

Type of Organisms require nutritional supplement and need special growth requirements

Answer 2

Fastidious (Slow grower)

Question 3

Fastidious organisms that are hemophilic?

Answer 3

Haemophilus spp.

Question 4

Fastidous organisms that are fermenters and non fermenters?

Answer 4

Haemophilus aphrophilus
Eikenella spp.
Capnocytophaga

Question 5

Fastidous organisms that are anaerobes?

Answer 5

Bacteroides
Fusobacterium
Prevotella spp

Question 6

What is the microscopical characteristics of haemophilus spp?

Answer 6

Gram negative pleomorphic coccobacilli / bacilli

Question 7

What is the characteristical growth of Haemophilus in CAP?

Answer 7

Rods with occasionally long filamentous rods

Question 8

Motility?

Answer 8

Nonmotile

Question 9

Unique characteristics of Haemophilus?

Answer 9

Obligate parasite

Question 10

What is obligate parasite?

Answer 10

Attaches to the mucous membrane

Question 11

All Haemophilus ferments Carbohydrates except?

Answer 11

H. ducreyi

Question 12

Oxidase and catalase?

Answer 12

positive

Question 13

Reduction of nitrate?

Answer 13

Positive

Question 14

What is the test use to differentiate Haemophilus spp?

Answer 14

X and V factor

Question 15

Meaning of X factor?

Answer 15

Hemin (Heat stable

Question 16

Meaning of V factor?

Answer 16

Nicotinamide Adenine Dinucleotide (Heat labile)

Question 17

All haemophilus spp with para- requires what factor?

Answer 17

V factor

Question 18

What ideal media can Haemophilus produce hemolysis?

Answer 18

Rabbit or horse blood agar

Question 19

What is the media of choice for Haemophilus?

Answer 19

CAP

Question 20

Why is CAP used instead of BAP?

Answer 20

To release X and V factor, V factor is heat labile which requires heating to release while X factors in intact in the RBC

Question 21

What is the Colony characteristic of haemophilus?

Answer 21

Satellitism

Question 22

What factor can Satellitism produce in?

Answer 22

V factor

Question 23

All haemophilus produce satellitism except?

Answer 23

H. aphrophilus (Does not grow in both X and V factor)

H. ducreyi (Only grows on X factor)

Question 24

Other organisms that can produce V factor?

Answer 24

S. aureus
Strep. pneumoniae
Neisseria spp.

Question 25

V factor positive in BAP if an organisms grows near the V factor producing organisms?

Answer 25

YES

Question 26

10% of Haemophilus are normal flora in?

Answer 26

URT (Adults)

Question 27

As children matures, Haemophilus normal flora spp converts ______________________ strains

Answer 27

encapsulated to nonencapsulated

Question 28

Strain that is involved in invasive infection

Answer 28

Encapsulated

Question 29

Strains usually involved in Localized infection

Answer 29

Nonencapsulated