3.2.EX Lab diag (All clostridium)

Question 1

Cellular morphology:
Gram Variable
Spores = Subterminal
Swell cells

Boxcar
Large square rods

Colonial Appearance:
Double Zone of hemolysis

Answer 1

Clostridium perfringens

Question 2

Cellular morphology:
Gram Variable
Swollen terminal spores (Tennis racket)

Drum stick or tack head bacillus appearance

Colony morphology:
Smoothly swarming but slow growing

Narrow zone of hemolysis?

Answer 2

C. tetani

Question 3

Gram positive

Spores that is oval or sub terminally located

Answer 3

Clostridium botulinum

Question 4

Thin Rods with rare spores

Has a Horse stable, barnyard odor

Yellow “Ground glass”

Answer 4

C. difficile

Question 5

Cellular morphology:
Thin rods
Subterminal

Colonial Morphology:
Resembles medusa head
Beta hemolytic
Smoothly swarming

Answer 5

C. septicum

Question 6

Swarming (-)
Double zone of Beta hemolysis (+)
Chartreuse Fluorescence (-)
Lecithinase (+)
Lipase (-)
Spore position (ST)

Answer 6

C. perfringens

Question 7

Swarming (-)
Double zone of Beta hemolysis (-)
Chartreuse Fluorescence (-)
Lecithinase (-)
Lipase (+)
Spore position (ST)

Answer 7

C. botulinum

Question 8

Swarming (+)
Double zone of Beta hemolysis (-)
Chartreuse Fluorescence (-)
Lecithinase (-)
Lipase (-)
Spore position (T)

Answer 8

C. tetani

Question 9

Swarming (-)
Double zone of Beta hemolysis (-)
Chartreuse Fluorescence (+)
Lecithinase (-)
Lipase (-)
Spore position (ST)

Answer 9

C. difficile

Question 10

Swarming (+)
Double zone of Beta hemolysis (-)
Chartreuse Fluorescence (-)
Lecithinase (-)
Lipase (-)
Spore position (ST)

Answer 10

C. septicum