2.4.2 Laboratory diagnosis Flashcards

1
Q

What is the specimen of choice for Bordetella?

A

Nasopharyngeal aspirates or swabs

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2
Q

What is the transport medium for the specimen of choice?

A

Calcium alginate and Dacron

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3
Q

NOTE: Throat swab can also be use for specimen collection

A
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4
Q

What are the transport medium used?

A

Amies with charcoal
1% Casein hydrolysate
Regan-Lowe transport medium

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5
Q

NOTE: Regan Lowe transport medium contains 10% horse blood and 40mg/L Cephalexin

A
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6
Q

What are the Agar media use?

A

Bordet-Gengou/Potato blood glycerol
Charcoal horse blood

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7
Q

Incubation?

A

Moist chamber at 35C > 7days

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8
Q

What is the microscopical morphology for Bordetella?

A

Tiny gram neg coccobacilli

Can also use Direct fluorescent antibody staining

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9
Q

What media is used to identify hemolytic for Bordetella?

A

Bordet gengou agar

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10
Q

What agar can give these characteristics?

Smooth, Glistening and silver, Resembing mercury droplets

A

Charcoal-horse blood and Regan Lowe media

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11
Q

How many days for the growth of B. pertussis in Charcoal Horse blood agar?

A

3-5 days

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12
Q

How many days for the growth of B. parapertussis in Charcoal Horse blood agar?

A

2-3 days

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13
Q

Charcoal horse blood (3-5 days)
Catalase (+)
Oxidase (+)
Urease (-)
Nitrate (-)
Motility (-)

A

B. pertussis

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14
Q

Charcoal horse blood (2-3 days)
Catalase (+)
Oxidase (-)
Urease (+)
Nitrate (-)
Motility (-)

A

B. parapertussis

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