Purification and Characterization of Proteins

Question 1

name the methods that separate proteins by size

Answer 1

• gel filtration
• dialysis & ultrafiltration

Question 2

name the methods that separate proteins by charge

Answer 2

• ion-exchange chromatography
• affinity chromatography
• HPLC (high performance liquid chromatography)

Question 3

name the method that separates proteins by purity and molecular weight

Answer 3

SDS-page

Question 4

name the method that determines the primary sequence of protein

Answer 4

Edman degradation