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Sem 3: BacT > Pseudomonas - Gutierrez > Flashcards

Pseudomonas - Gutierrez Flashcards

1
Q

Does psuedomonas form spores?

A

no

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2
Q

Can pseudomonas spp ferment lactose?

A

No

Oxidase +

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3
Q

Pseudomonas are _________ pathogens

A

Opportunistic

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4
Q

What is the reservoir for pseudomonas?

A

Environment - water, soil, plants

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5
Q

Pseudomonas aeruginosa is associated with what predisposing factors?

A
  1. Trauma, burns, wounds
  2. Malignancy
  3. Immunodeficiency
  4. Inbalance of microbiota
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6
Q

True or False: Pseudomonas is involved in primary disease

A

False, secondary to other causes

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7
Q

What is responsible for the blue- green color on culture of pseudomonas aeruginosa? What smell is associated?

A

Pyocyanin (siderophore), has a candy-sweet odor

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8
Q

What virulence factors are on the cell surface of pseudomonas aeruginosa?

A

Flagella, pili, LPS

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9
Q

What is responsible for the fluorescent green color on culture of pseudomonas aeruginosa?

A

Pyoverdin siderophore

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10
Q

What is responsible for the red-brown color on culture of pseudomonas aeruginosa?

A

Pyorubin siderophore

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11
Q

What is responsible for the brown/black color on culture of pseudomonas aeruginosa?

A

Pyomelanin siderophore

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12
Q

How is pseudomonas aeruginosa transmitted?

A

Indirect contact through contaminated tools, water bowls, bedding, thermometers, etc

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13
Q

What are the 3 stages involved in pseudomonas aeruginosa?

A
  1. Adherence + colonization
  2. Multiplication and growth (biofilm formation)
  3. Systemic disease
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14
Q

What toxins are involved in systemic pseudomonas aeruginosa disease?

A

LPS and EXOtoxin A

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15
Q

What happens during growth and multiplication of pseudomonas aeruginosa?

A

bio film is formed, quorum sensing, T3SS is expressed

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16
Q

How does pseudomonas aeruginosa adhere to host tissue?

A

Flagella and pili

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17
Q

What are the major pathologies associated with pseudomonas aeruginosa in many species of animals?

A
  1. Wound infections
  2. UTI
  3. Otitis externa + media
  4. Resp tract infection
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18
Q

pseudomonas aeruginosa can cause abortion in what species?

A

Horses and chinchillas

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19
Q

What CS are associated with dogs and cats with pseudomonas aeruginosa?

A
  1. Otitis externa - green pus, malodorous discharge
  2. Lower UTI
  3. Pneumonia
  4. Pyoderma
20
Q

What CS are seen in horses with pseudomonas aeruginosa?

A
  1. Infectious endometritis
  2. Vaginitis
  3. Abortion
  4. Conjunctivitis
21
Q

What CS are seen in cows with pseudomonas aeruginosa?

A

Subclinical mastitis

Elevated somatic cell count in more chronic infections

Can have resp infection, uterine infection, enteritis

22
Q

What CS are seen in SHOATS with pseudomonas aeruginosa?

A

Gangrenous mastitis - high fatality rates

Fleece rot - superficial dermatitis, “green wool”

23
Q

Gangrenous mastitis is associated with what animal species and what bacteria?

A

SHOATS, pseudomonas aeruginosa

24
Q

What CS are seen in rabbits with pseudomonas aeruginosa?

A
  1. Dermatitis
  2. Pneumonia
  3. Moist skin from automated water system
25
Q

The origin of pseudomonas aeruginosa comes from __________

A

contaminated drinking water

26
Q

What CS are seen in mink with pseudomonas aeruginosa?

A
  1. Pulmonary lesions - pneumonia
  2. High mortality
27
Q

What CS are seen in chinchillas with pseudomonas aeruginosa?

A
  1. Abortion
  2. Septicemia
  3. Pneumonia
  4. Otitis media
  5. Sudden death
28
Q

What CS are seen in reptiles with pseudomonas aeruginosa??

A
  1. Necrotic stomatits, “mouth rot”
  2. Ulcers on skin
  3. Pneumonia
  4. Septicemia
29
Q

Reptiles are often infected with pseudomonas aeruginosa secondary to ___________

A

poor sanitary housing conditions

30
Q

What pseudomonas spp are fish most at risk for?

A

Pseudomonas fluorescens

31
Q

__________ is important in the spoilage of refrigerated milk, meat, eggs, seafood, and blood products

A

P. Fluorescens

32
Q

Rainbow trout with ulceration on the dorsal surface is associated with what bacteria spp?

A

Pseudomonas putida

33
Q

Who can be infected with pseudomonas fluorescens?

A

Fish - most pathogenic fish pathogen

Poulty

Cattle

Humans (zoonotic)

34
Q

Which pseudomonas spp grow at 5 °C?

A

P. Fluorescens

35
Q

How can pseudomonas be prevented?

A

Prevent wet conditions

Vaccine available only for minks

36
Q

Mouth rot is characteristic of ________ bacteria spp in ________ animals

A

Pseudomonas aeruginosa, reptiles

37
Q

P. fluorescens causes _______ in cattle

38
Q

P. fluorescens causes _______ in fish

A

Septicemia and tail/fin rot

39
Q

P. fluorescens causes _______ in poultry

A

Embryonic mortality

40
Q

Exophthalmia, ascites, and ulceration in tilapia is characteristic of ________ bacteria spp

A

Pseudomonas putida

41
Q

What does pseudomonas look like on macconkey agar?

A

Yellow bc does not ferment lactose

  • pale colonies with green-blue pyocyanin pigment
42
Q

True or False: Penicillin is used to treat pseudomonas

A

NOOOOO FALSE

resistant to alot of broad spectrum antibiotics

43
Q

P. aeruginosa can cause necrotic stomatitis in ________

A

reptiles / snakes

44
Q

P. aeruginosa can cause dermatitis in __________

A

Sheep and rabbits

45
Q

P. aeruginosa can cause endocarditis in ____________

A

Dogs and cats

Sem 3: BacT (16 decks)

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