Bacterial Diagnosis - Muller

Question 1

What stain should be used for Bacillus anthracis?

Answer 1

Polychrome methylene blue

Question 2

What stain should be used for Brucella abortus?

Answer 2

Modified Ziehl-Nielsen stain

Question 3

What stain should be used for Mycoplasma haemofelis?

Answer 3

Giemsa / Diff quick

Question 4

What stain should be used for Campylobacter?

Answer 4

DCF - dilute carbol fuchsin

Question 5

Which stain should be used for Mycobacterium?

Answer 5

Ziehl-Nielson stain

Question 6

What is the GOLD STANDARD for diagnosis of many bacterial diseases?

Answer 6

CULTURE

Question 7

What culture media is selective for Gram + bacteria?

Answer 7

Phenylethyl Alcohol agar (PEA)

Question 8

What culture media is selective for Gram - bacteria?

Answer 8

Mac Conkey agar

Question 9

Why would a differential culture media be used?

Answer 9

To distinguish 1 bacteria growing on the same plate from another bacteria

Question 10

What are the limitations of serology?

Answer 10

False negs - due to recent exposure or immunosupression (Not enough Ab to see a positive result)

OR

False positives - due to vaccination, cross rxns, or past infection

Question 11

What are the advantages of serology?

Answer 11

1. Useful for chronic and carrier state
2. Used for screening populations (Ex: large herds)
3. Rapid tests available