Proteins 2

Question 1

Key control point for gylcolysis

Answer 1

Phosphofructokinase-1

Question 2

Immunoglobulin G

Subunits/Subtypes

Prefixes

Answer 2

4/2

y2k2

Question 3

Structure Determination Tools

Answer 3

X- Ray Crystallography

Nuclear Magnetic Resonance

Question 4

Lactate Dehydrogenase

Subunits/Subtypes

Prefixes

Answer 4

4/2

H₄, M₄, H₂M₂, HM₃, H₃M

Question 5

Examples of TIM barrel fold

Answer 5

aldolase

enolase

pyruvate kinase

Question 6

Myoglobin stores ___ in the ___.

Answer 6

O2; muscle

Question 7

Phosphorylase b Kinase

Subunits/Subtypes

Prefixes

Answer 7

16/4

a₄B₄y₄delta₄

Question 8

Primary role of the Helix-Loop-Helix motif

Answer 8

Binds DNA and directs transcription

Question 9

Supersecondary structure

Answer 9

compact three-dimensional protein structure of several adjacent elements of a secondary structure that is smaller than a protein domain or a subunit.

Question 10

Amino acids included in the immunoglobulin fold

Answer 10

Tyrosine

Question 11

Quaternary structures are stabilized by_____forces only

Answer 11

Noncovalent

Question 12

Hemoglobin

Subunits/Subunit tyoes

Prefixes

Answer 12

4/2

a₂B₂

Question 13

–_______ or _________: combinations of 2° structural elements; small, nonrepeating structural units commonly found in proteins

–___________: a structurally-independent folding unit within a protein; acts as single entity

Answer 13

Motifs; Folds

Domain

Question 14

______________is a ~ 400 kDa protein that has an extensive antiparallel b-sheet structure.

Answer 14

•Silk fibroin

Question 15

Examples of Immunoglobulin Fold

Answer 15

Titin

Cadherins

Question 16

Examples of the helix-loop-helix motif

Answer 16

MyoD

Question 17

What side chains make up the Helix-Loop-Helix Motif?

Answer 17

Basic side chains

Question 18

Myoglobin structure

Answer 18

O2 in muscle

Question 19

Asparate Transcarbanylase

Subunits/Subtypes

Prefixes

Answer 19

12/2

a₆B₆

Question 20

TIM Barrel Fold contains alternating segments of ____ and ____.

Answer 20

Alpha helices and Beta strands

Question 21

Examples of the Zinc- Finger DNA Binding Motif?

Answer 21

Xenopus laevis

TFIIIA

Question 22

What side chains are included in the Zinc-Finger DNA Binding Motif? What’s the ratio?

Answer 22

2 Cys, 2 His or 4 Cys

Question 23

_______ and __________ are common in b-turns but tend to disrupt the a-helix

Answer 23

Glycine; proline

Question 24

How many angstroms are in one rise/residue?

Answer 24

1.5

Question 25

B loops are located on the __ of proteins

Answer 25

Surface

Question 26

__ residues required to make a turn

Answer 26

4

Question 27

The ________ beta sheet often requires alpha helices to connect.

Answer 27

Parallel

Question 28

Are B-strands stable by themelves?

Answer 28

No. Only when incorporated into sheets

Question 29

Proteins can evolve via____________ mutation

Answer 29

Genetic

Question 30

Example protein with antiparallel B sheet structure

Answer 30

Fibroin

Question 31

Example of a supersecondary molecule.

Answer 31

The Immunoglobulin Fold or Domain

Question 32

•The________ are not stable by themselves, but only when incorporated into sheets.

Answer 32

Beta strands

Question 33

What role does Zn2+ play in the Zinc -Finger DNA Binding Motif?

Answer 33

Stabilizes the structure

Question 34

Phosphofructokinase-1

• _________of four identical subunits
• Four ______sites and four ________sites
• _________enzyme: Allosterically inhibited by _______ and_______
• activated by ________ and ___________
• KEY CONTROL POINT FOR__________

Answer 34

Tetramer

Catalytic; regulatory

Enzyme; ATP; Citrate

AMP; Fructose- 2,6 bisphosphate

Glycolysis