Protein stabilisation + assisted protein folding Flashcards
1
Q
Once folding has occured, what stabilises the protein further
A
Disulfide bonds between cysteine bonds
2
Q
what are molecular chaperones
A
Specialised proteins that facilitate folding when needed
3
Q
Name 3 chaperones
A
Hsp70 (DnaK)
Hsp40 (DnaJ)
GrpE
4
Q
Name the chaperoins
A
GroES and GroEL
5
Q
What does protein disulfide isomerase (PDI) do
A
catalyses the interchange or shuffling of disulfide bonds until the native configuration of disulfide bonds is achieved.
catalyses elimination of folding intermediates with inappropriate disulfide cross link
6
Q
What does peptide prolyl cis-trans isomerase (PPI) do
A
Catalyses the interconversion of the cis and trans isomers of proline peptides bonds
7
Q
How can the function of a protein be lost
A
misfolding of the protein
- mad cows diease
