Chymotrypsin Flashcards
1
Q
What is the key active site residue chymotrypsin?
A
Ser 195
2
Q
What is the catalytic triad of chymotrypsin
A
Ser 195
His 57
Asp 102
3
Q
What does Ser195 do?
A
Carries out nucleophilic attack, due to OH side chain
4
Q
What does His57 do? (Imidazoble group)
A
Acts as a base catalyst and accepts a proton from Ser 195
5
Q
What does Asp102 do? (Carbox acid)
A
Hydrogen bonds to His57 which increases its pKa to make it a better catalyst
