OPTICAL MICROSCOPES Flashcards

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1
Q

how to prepare a temporary mount

A
  1. pipette a drop of water onto the slide
  2. use tweezers to place a thin layer of cells / thin section of specimen onto the slide
  3. add a drop of stain
  4. lower a coverslip over the specimen using a mounted needle. the cover slip protects the specimen making sure no air bubbles get in
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2
Q

about light microscopes

A

light passes through specimen and is focused through glass lenses, which project a magnified image
max resolution of 0.2 micrometers
cant view smaller organelles
specimen must be thin so light can pass through
stains e.g. KI make certain structures more visible

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3
Q

advantages of light microscopes

A

can view live specimens
can show colour
less time consuming to prepare slides

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4
Q

disadvantages of light microscope

A

lower resolution compared to elec
lower magnification compared to elec
cant view smaller organelles in detail

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5
Q

what is an eyepiece graticule

A

scale in the eyepiece typically 10mm long and is divided into 100 sub sections

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6
Q

what is a stage micrometer

A

a slide with a scale etched on, 10mm long with 1mm sub divisions and the smallest subdivisions of 0.1mm

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