Nonenteric Gram Neg. Bacilli

Question 1

NonEnteric Fermenters:

-Organisms

Answer 1

• Vibrio cholerae
• V. parahemolyticus
• V. vulnificus
• Aeromonas/Plesiomonas

Question 2

NonEnteric Fermenters:

-General features

Answer 2

• Growth on MacConkey
• Glucose Fermentation
• Oxidase POSITIVE

Question 3

V. cholerae:

• Agar
• Colony feature
• Sucrose
• Other test

Answer 3

V. cholerae:

• TCBS Agar
• YELLOW colonies
• Sucrose POSITIVE
• String Test POSITIVE

Question 4

V. parahemolyticus:

• Agar
• Colony feature
• Sucrose

Answer 4

V. parahemolyticus:

• TCBS Agar
• GREEN colonies
• Sucrose NEGATIVE

Question 5

V. vulnificus:

• Enviroment/Geographic
• Symptoms

Answer 5

• Warm, low salinity marine environments (Gulf of Mexico)

- Vomiting AND Diarrhea

Question 6

Aeromonas/Plesiomonas:

• Environment
• Pathogenecity

Answer 6

• Fresh/Brackish waters (Hospital/Fish Tanks)

- Not all strains are pathogenic

Question 7

What Vibrio spp. may cause skin infections?

Answer 7

V. parahemolyticus and V. vulnificus

Question 8

What Vibrio spp. is the most common cause of foodborne illness in Japan?

Answer 8

V. parahemolyticus (Seafood)

Question 9

Pseudomonas aeruginosa:

• MacConkey (+/-)
• Glucose Fermentation (+/-)

Answer 9

Grows on MacConkey

-Does NOT ferment Glucose

Question 10

Pseudomonas aeruginosa:

• Oxidase
• Motile (+/-)

Answer 10

Pseudomonas aeruginosa:

• Oxidase POSITIVE
• Motile - polar monotrichous flagella

Question 11

Pseudomonas aeruginosa:

• Temperature characteristic
• Hemolysis
• Odor

Answer 11

Pseudomonas aeruginosa:

• Grows well at 42C (culture/environment)
• Beta-Hemolysis
• “Grape-like” odor

Question 12

Pseudomonas aeruginosa:

-Colonies produce what?

Answer 12

Green diffusible pigment

• Few strains produce:
• Red (pyorubicin)
• Black (pyomelanin)

Question 13

Pseudomonas aeruginosa:

-Clinical Complications

Answer 13

Cystic Fibrosis

Endocarditis (IV Drugs)